epiglucan and acetophenone

The aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/mL or 20 μg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant.. The relative expression of interleukin (IL)-1-α and IL-18 genes in HGF-1 decreased with 10 μg/mL or 20 μg/mL of β-glucan, where as the expression of PTGS-2 decreased only with 10 μg/mL. The expression of IL-1-α increased with 20 μg/mL and that of IL-18 increased with 10 μg/mL in OBA-9; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of β-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 μg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 μg/mL of β-glucan.. Treatment with β-glucans positively modulated the immune response and production of metabolites.

Topics: Acetophenones; Aggregatibacter actinomycetemcomitans; Anti-Infective Agents; Benzoic Acid; beta-Glucans; Cell Culture Techniques; Cell Line; Coculture Techniques; Cyclooxygenase 2; Cytokines; E1A-Associated p300 Protein; Fibroblasts; gamma-Aminobutyric Acid; Gene Expression; Host-Pathogen Interactions; Humans; Hydroxybutyrates; Immunomodulation; Inositol; Interleukin-18; Interleukin-1alpha; Keratinocytes; Lymphoma, B-Cell; Metabolome; Mouth; Oxalic Acid; Periodontal Diseases; Proto-Oncogene Proteins c-bcl-2; Real-Time Polymerase Chain Reaction