epidermal-growth-factor and hydroxyethyl-methacrylate

This study addresses a variety of immunohistochemical conditions for detecting EGF in 3.5% paraformaldehyde fixed, glycol methacrylate embedded tissue including antigen unmasking with trypsin, dilution of primary antibody, and incubation time with primary antibody. Color development was achieved with a biotinylated secondary antibody linked to an avidin biotinylated peroxidase complex. Trypsinization and a 12 hr incubation with the primary antibody was essential to detect EGF in this system. Adequate staining could be achieved with a 1:100 dilution of the primary antibody.

Topics: Animals; Epidermal Growth Factor; Immunohistochemistry; Male; Methacrylates; Mice; Plastic Embedding; Staining and Labeling; Submandibular Gland; Trypsin