epidermal-growth-factor and 4-diphenylacetoxy-1-1-dimethylpiperidinium

epidermal-growth-factor has been researched along with 4-diphenylacetoxy-1-1-dimethylpiperidinium* in 1 studies

Other Studies

1 other study(ies) available for epidermal-growth-factor and 4-diphenylacetoxy-1-1-dimethylpiperidinium

Article	Year
Activation of mitogen-activated protein kinase by cholinergic agonists and EGF in human compared with rat cultured conjunctival goblet cells. Investigative ophthalmology & visual science, 2003, Volume: 44, Issue:6 To compare activation of the p42/p44 mitogen-activated protein kinase (MAPK) by cholinergic agonists and epidermal growth factor (EGF) in cultured human and rat goblet cells.. . Conjunctiva was removed from either humans during ocular surgery or male Sprague-Dawley rats and cultured in RPMI medium. These cells were incubated with the cholinergic agonist carbachol (10(-4) M) or EGF (10(-8) M) for various times. Before stimulation, cells were incubated with the EGF receptor (EGFR) inhibitor, AG1478 (10(-7) M) or the muscarinic M(3) receptor inhibitor, 4-diphenylacetoxy-N-(2-chloroethyl)-piperidine hydrochloride (4-DAMP; 10(-5) M) for 10 minutes. Proteins were analyzed by Western blot analysis, using antibodies specific to phosphorylated (activated) p42/44-MAPK or total p42-MAPK. Immunoreactive bands were quantified, and data were expressed as percentage of increase over basal.. Carbachol (10(-4) M) increased MAPK activity in human and rat cultured goblet cells in a time-dependent manner, increasing pMAPK with a maximum at 10 minutes. EGF (10(-8) M) activated MAPK in human and rat goblet cells in a time-dependent manner with a maximum at 5 minutes. Carbachol- and EGF-induced activation of pMAPK was completely inhibited by AG1478 in cultured conjunctival goblet cells from both species. Carbachol-induced MAPK activity was also completely inhibited by 4-DAMP in both species.. In human and rat cultured conjunctival goblet cells, cholinergic agonists and EGF activate MAPK with a similar time dependency, this activation is receptor mediated, and cholinergic agonists transactivate the EGF receptor. Thus, rat cultured conjunctival goblet cells can be used as a model to study human conjunctival goblet cells. Topics: Adult; Aged; Aged, 80 and over; Animals; Blotting, Western; Carbachol; Cells, Cultured; Cholinergic Agonists; Conjunctiva; Enzyme Activation; Enzyme Inhibitors; Epidermal Growth Factor; ErbB Receptors; Goblet Cells; Humans; Male; Middle Aged; Mitogen-Activated Protein Kinases; Muscarinic Antagonists; Piperidines; Quinazolines; Rats; Rats, Sprague-Dawley; Time Factors; Tyrphostins	2003

Article

Year

Activation of mitogen-activated protein kinase by cholinergic agonists and EGF in human compared with rat cultured conjunctival goblet cells.

Investigative ophthalmology & visual science, 2003, Volume: 44, Issue:6

To compare activation of the p42/p44 mitogen-activated protein kinase (MAPK) by cholinergic agonists and epidermal growth factor (EGF) in cultured human and rat goblet cells.. . Conjunctiva was removed from either humans during ocular surgery or male Sprague-Dawley rats and cultured in RPMI medium. These cells were incubated with the cholinergic agonist carbachol (10(-4) M) or EGF (10(-8) M) for various times. Before stimulation, cells were incubated with the EGF receptor (EGFR) inhibitor, AG1478 (10(-7) M) or the muscarinic M(3) receptor inhibitor, 4-diphenylacetoxy-N-(2-chloroethyl)-piperidine hydrochloride (4-DAMP; 10(-5) M) for 10 minutes. Proteins were analyzed by Western blot analysis, using antibodies specific to phosphorylated (activated) p42/44-MAPK or total p42-MAPK. Immunoreactive bands were quantified, and data were expressed as percentage of increase over basal.. Carbachol (10(-4) M) increased MAPK activity in human and rat cultured goblet cells in a time-dependent manner, increasing pMAPK with a maximum at 10 minutes. EGF (10(-8) M) activated MAPK in human and rat goblet cells in a time-dependent manner with a maximum at 5 minutes. Carbachol- and EGF-induced activation of pMAPK was completely inhibited by AG1478 in cultured conjunctival goblet cells from both species. Carbachol-induced MAPK activity was also completely inhibited by 4-DAMP in both species.. In human and rat cultured conjunctival goblet cells, cholinergic agonists and EGF activate MAPK with a similar time dependency, this activation is receptor mediated, and cholinergic agonists transactivate the EGF receptor. Thus, rat cultured conjunctival goblet cells can be used as a model to study human conjunctival goblet cells.

Topics: Adult; Aged; Aged, 80 and over; Animals; Blotting, Western; Carbachol; Cells, Cultured; Cholinergic Agonists; Conjunctiva; Enzyme Activation; Enzyme Inhibitors; Epidermal Growth Factor; ErbB Receptors; Goblet Cells; Humans; Male; Middle Aged; Mitogen-Activated Protein Kinases; Muscarinic Antagonists; Piperidines; Quinazolines; Rats; Rats, Sprague-Dawley; Time Factors; Tyrphostins

2003