epidermal-growth-factor and 3-4-dehydroproline

The proline analogs cis-4-hydroxy-L-proline, 3,4-dehydro-L-proline, and 2-azetidinecarboxylic acid induced increases in the migration rate of retinal capillary endothelial cells, aortal endothelial cells, corneal endothelial cells, aortal smooth muscle cells, and retinal pericytes. cis-4-hydroxy-D-proline did not. The optimal concentration for migration induction by any of the active agents was approximately 10(-5) M. At higher concentrations (5 x 10(-4) M) migration was not induced or was inhibited. When tested by subcutaneous implant assays in rabbits, cis-4-hydroxy-L-proline and 2-azetidinecarboxylic acid consistently elicited a marked angiogenic response. Whereas these compounds are known to modulate collagen synthesis and secretion, the concentrations at which they are effective inducers of migration suggest that they may have a more specific target than general collagen synthesis.

Topics: Animals; Azetidinecarboxylic Acid; Azetines; Cattle; Cell Division; Cell Movement; Cells, Cultured; Collagen; Drug Implants; Endothelium; Epidermal Growth Factor; Hydroxyproline; Neovascularization, Pathologic; Procollagen-Proline Dioxygenase; Proline