epidermal-growth-factor and 1-2-distearoyllecithin

Lipodisks are nanosized flat, circular, phospholipid bilayers that are edge-stabilized by polyethylene glycol-conjugated lipids (PEG-lipids). Over the last decade, lipodisks stabilized with PEG of molecular weight 2000 or 5000 have been shown to hold high potential as both biomimetic membranes and drug carriers. In this study we investigate the possibilities to optimize the properties of the lipodisks, and widen their applicability, by reducing the PEG molecular weight and/or the density of the PEG corona. Results obtained by cryo-transmission electron microscopy and dynamic light scattering show that stable, well-defined lipodisks can be produced from mixtures of distearoylphosphatidylcholine (DSPC) and distearoylphosphatidylethanolamine conjugated to PEG of molecular weight 1000 (DSPE-PEG

Topics: Cell Line, Tumor; Drug Carriers; Epidermal Growth Factor; Epithelial Cells; ErbB Receptors; Gene Expression; Humans; Iodine Radioisotopes; Lipid Bilayers; Molecular Targeted Therapy; Molecular Weight; Phosphatidylcholines; Phosphatidylethanolamines; Polyethylene Glycols; Porosity; Protein Binding; Silicon Dioxide; Structure-Activity Relationship