Compounds > enkephalin--ala(2)-mephe(4)-gly(5)-

enkephalin--ala(2)-mephe(4)-gly(5)- and tyrosyl-alanyl-glycyl-phenylalanyl-leucine-chloromethyl-ketone

enkephalin--ala(2)-mephe(4)-gly(5)- has been researched along with tyrosyl-alanyl-glycyl-phenylalanyl-leucine-chloromethyl-ketone* in 1 studies

Other Studies

1 other study(ies) available for enkephalin--ala(2)-mephe(4)-gly(5)- and tyrosyl-alanyl-glycyl-phenylalanyl-leucine-chloromethyl-ketone

Article	Year
Identification of an opioid receptor subunit carrying the mu binding site. Biochemistry, 1984, Nov-06, Volume: 23, Issue:23 The enkephalin affinity reagent [3H]Tyr-D-Ala-Gly-Phe-Leu-CH2Cl [( 3H]DALECK) was synthesized. It exhibited high-affinity reversible binding, at pH 7.4, to both mu and delta opioid receptor sites in rat brain membranes. At pH 8.1, nanomolar levels of [3H]DALECK produced an irreversible labeling in synaptic membranes, essentially only in one subunit of 58 000 daltons. The irreversible phase of the reaction reduced the subsequent binding of a mu-selective enkephalin derivative but not that of a delta-selective one. It is concluded that a mu subunit of the opioid receptor exists, can be alkylated specifically, and is of Mr 58 000. Topics: Affinity Labels; Alkylation; Amino Acid Chloromethyl Ketones; Animals; Binding Sites; Brain; Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; Enkephalin, Leucine; Enkephalin, Leucine-2-Alanine; Enkephalins; Hydrogen-Ion Concentration; Intracellular Membranes; Microsomes; Mitochondria; Molecular Weight; Rats; Receptors, Opioid; Synaptic Membranes	1984

Article

Year

Identification of an opioid receptor subunit carrying the mu binding site.

Biochemistry, 1984, Nov-06, Volume: 23, Issue:23

The enkephalin affinity reagent [3H]Tyr-D-Ala-Gly-Phe-Leu-CH2Cl [( 3H]DALECK) was synthesized. It exhibited high-affinity reversible binding, at pH 7.4, to both mu and delta opioid receptor sites in rat brain membranes. At pH 8.1, nanomolar levels of [3H]DALECK produced an irreversible labeling in synaptic membranes, essentially only in one subunit of 58 000 daltons. The irreversible phase of the reaction reduced the subsequent binding of a mu-selective enkephalin derivative but not that of a delta-selective one. It is concluded that a mu subunit of the opioid receptor exists, can be alkylated specifically, and is of Mr 58 000.

Topics: Affinity Labels; Alkylation; Amino Acid Chloromethyl Ketones; Animals; Binding Sites; Brain; Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; Enkephalin, Leucine; Enkephalin, Leucine-2-Alanine; Enkephalins; Hydrogen-Ion Concentration; Intracellular Membranes; Microsomes; Mitochondria; Molecular Weight; Rats; Receptors, Opioid; Synaptic Membranes

1984