endothelin-1 and azidopine

endothelin-1 has been researched along with azidopine* in 1 studies

Other Studies

1 other study(ies) available for endothelin-1 and azidopine

Article	Year
Endothelin-1 significantly increased number of specific high-affinity 1,4-dihydropyridine (DHP) binding sites photolabelled on vascular smooth muscle cells with (-)-[3H]-azidopine. Physiological research, 1996, Volume: 45, Issue:1 The effects of endothelin-1 (ET-1) on surface membrane Ca2+ channels were studied on cultured human embryonal vascular smooth muscle cells (VSMC) and on isolated rat aorta using photoaffinity labelling with DHP Ca2+ channel antagonist (-)-[3H]-azidopine (AZI). The AZI-labelled saturable population of sites on VSMC with Bmax = 1.59 +/- 0.10 pmol/mg of protein and KD = 5.40 +/- 1.70 nmol/l; and 1.32 +/- 0.11 pmol/mg w.w. and KD = 1.09 +/- 0.20 nmol/l in isolated rings of the rat aorta. Preincubation with ET-1 (0.1, 1.0 and 10 nmol/l) increased (in a concentration-dependent manner) the total number of sites specifically photolabelled on VSMC. The number of sites labelled with AZI on ET-1 preincubated VSMC increased markedly when divalent cations (Ca2+ or Mg2+ in other experiments) were present in the incubation medium. Specific photolabelling also significantly increased in VSMC pretreated with intrinsically photoreactive nifedipine. A protein kinase C inhibitor staurosporine, added to the incubation medium, significantly reduced the enhanced specific photolabelling after ET-1. The increase in specific photolabelling after ET-1 preincubation (+197 +/- 46%; P < 0.05) was also observed in rings of the rat aorta and it was significantly reduced after preincubation with S-(+)-niguldipine. Topics: Affinity Labels; Animals; Aorta, Thoracic; Azides; Binding Sites; Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Cells, Cultured; Dihydropyridines; Endothelin-1; Enzyme Inhibitors; Humans; In Vitro Techniques; Kinetics; Muscle, Smooth, Vascular; Protein Kinase C; Rats; Staurosporine	1996

Article

Year

Endothelin-1 significantly increased number of specific high-affinity 1,4-dihydropyridine (DHP) binding sites photolabelled on vascular smooth muscle cells with (-)-[3H]-azidopine.

Physiological research, 1996, Volume: 45, Issue:1

The effects of endothelin-1 (ET-1) on surface membrane Ca2+ channels were studied on cultured human embryonal vascular smooth muscle cells (VSMC) and on isolated rat aorta using photoaffinity labelling with DHP Ca2+ channel antagonist (-)-[3H]-azidopine (AZI). The AZI-labelled saturable population of sites on VSMC with Bmax = 1.59 +/- 0.10 pmol/mg of protein and KD = 5.40 +/- 1.70 nmol/l; and 1.32 +/- 0.11 pmol/mg w.w. and KD = 1.09 +/- 0.20 nmol/l in isolated rings of the rat aorta. Preincubation with ET-1 (0.1, 1.0 and 10 nmol/l) increased (in a concentration-dependent manner) the total number of sites specifically photolabelled on VSMC. The number of sites labelled with AZI on ET-1 preincubated VSMC increased markedly when divalent cations (Ca2+ or Mg2+ in other experiments) were present in the incubation medium. Specific photolabelling also significantly increased in VSMC pretreated with intrinsically photoreactive nifedipine. A protein kinase C inhibitor staurosporine, added to the incubation medium, significantly reduced the enhanced specific photolabelling after ET-1. The increase in specific photolabelling after ET-1 preincubation (+197 +/- 46%; P < 0.05) was also observed in rings of the rat aorta and it was significantly reduced after preincubation with S-(+)-niguldipine.

Topics: Affinity Labels; Animals; Aorta, Thoracic; Azides; Binding Sites; Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Cells, Cultured; Dihydropyridines; Endothelin-1; Enzyme Inhibitors; Humans; In Vitro Techniques; Kinetics; Muscle, Smooth, Vascular; Protein Kinase C; Rats; Staurosporine

1996