endothelin-1 and 11-mercaptoundecanoic-acid

We report results of the studies relating to the fabrication of a surface plasmon resonance (SPR) based label-free immunosensor for real-time monitoring of endothelin-1 (ET-1), a colon cancer biomarker. A gold disk modified with a self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid (11-MUA) was functionalised via covalent immobilization of monoclonal anti-ET-1 antibodies using EDC-NHS (1-(3-(dimethylamine)-propyl)-3-ethylcarbodiimide hydrochloride, N-hydroxy succinimide) chemistry. This immunosensing platform (ethanolamine/anti-ET-1/11-MUA/Au) was characterized via atomic force microscopy (AFM), contact angle (CA) and Fourier transform infrared (FT-IR) spectroscopic techniques. The fabricated SPR electrode was further used to detect ET-1 in the broad concentration range 2-100 pg mL

Topics: Antibodies, Immobilized; Antibodies, Monoclonal; Biomarkers, Tumor; Biosensing Techniques; Colonic Neoplasms; Electrochemical Techniques; Electrodes; Endothelin-1; Fatty Acids; Gold; Humans; Kinetics; Limit of Detection; Sulfhydryl Compounds; Surface Plasmon Resonance