eleostearic-acid and sodium-arsenite

The aim of the present study was to investigate the protective effect of conjugated linolenic acid (CLnA), present in vegetable oils against arsenite-induced renal oxidative stress.. Albino rats were divided into six groups. Group 1 was control and group 2 was treated with sodium arsenite (Sa; 10 mg/kg BW). Rats in groups 3 and 4 were treated with mixture of α-eleostearic acid and punicic acid (1:1) (0.5% and 1.0%, respectively), whereas rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic acid and 0.5% of punicic acid, respectively, along with Sa by oral gavage once daily.. Results revealed that activity of antioxidant enzymes and total reduced glutathione content, total protein content, and phospholipid content in kidney were decreased significantly in arsenite-treated group compared with control. Activity of nitric oxide synthase, peroxidation of lipid, protein oxidation, total cholesterol content, total lipid content of kidney, and plasma creatinine level were increased significantly (P < 0.05) in arsenite-treated rats compared with control. Fatty-acid composition of renal lipids showed significant decrease in monounsaturated fatty acid, polyunsaturated fatty acid (PUFA) content, and increase in saturated fatty acid content due to oxidative stress. PUFA such as γ-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid decreased significantly with significant (P < 0.05) increase in arachidonic acid content after Sa treatment. Administration of blended product of both the isomers caused better restoration of renal fatty acids and other altered parameters.. CLnA isomers caused amelioration of renal oxidative stress and the isomers showed synergistic activity.

Topics: Animals; Antioxidants; Arachidonic Acid; Arsenites; Creatinine; Dietary Fats; Docosahexaenoic Acids; Eicosapentaenoic Acid; gamma-Linolenic Acid; Glutathione; Isomerism; Kidney; Linolenic Acids; Lipid Metabolism; Liver; Male; Nitric Oxide Synthase; Oxidative Stress; Plant Oils; Rats; Sodium Compounds

The purpose of the present study was to examine the antioxidant activity of two typical oils obtained from two vegetables, bitter gourd seed and snake gourd seed, containing two different isomers of conjugated linolenic acid (CLnA) against oxidative stress induced by sodium arsenite in relation to tissue lipid peroxidation and inflammation. Male albino rats were taken as subject and divided into six groups: Group 1 was control and Group 2 was treated with sodium arsenite (Sa; 10mg/Kg BW); Groups 3-6 were orally treated with different doses of seed oils maintaining definite concentration of CLnA isomers (0.5% and 1.0% of total lipid for each CLnA isomer) along with sodium arsenite. There was significant increase in lipid peroxidation, pro-oxidant enzyme activity and decrease in antioxidant enzyme activity in brain due to Sa administration. Decrease in total protein content was also observed in plasma, liver and brain of Sa treated group. Significant decrease in phospholipid content and increase in total lipid content and cholesterol content were observed in arsenite treated group. There was significant increase in relative organ weight of liver due to Sa administration. Fatty acid profile of liver and brain lipid shows significant (P<0.05) reduction in most of the polyunsaturated fatty acids and increase in arachidonic acid (20:4n-6) (75.23%) due to inflammation after arsenite treatment. Administration of experimental oils made almost complete restoration of those altered parameters. Overall, these two oils were effective in protecting tissue lipid profiles which were altered due to oxidative stress.

Topics: Administration, Oral; alpha-Linolenic Acid; Animals; Anti-Inflammatory Agents; Antioxidants; Arachidonic Acid; Arsenites; Brain; Catalase; Disease Models, Animal; Fatty Acids, Unsaturated; Glutathione Peroxidase; Inflammation; Isomerism; Linolenic Acids; Lipid Peroxidation; Liver; Male; Oxidative Stress; Phospholipids; Plant Oils; Rats; Sodium Compounds; Superoxide Dismutase

The present study was undertaken to evaluate the antioxidant efficacy of alpha-eleostearic acid and punicic acid, two isomers of conjugated linolenic acid, in terms of normalization of altered biochemical parameters of oxidative stress following sodium arsenite treatment in rats. Animals were divided into four groups. The first group used as control. While, group 2, 3 and 4 were orally treated with alpha-eleostearic acid (0.5% of total lipid given) plus sodium arsenite (Sa; 10mg/kgBW), punicic acid (0.5% of total lipid given) plus sodium arsenite (Sa; 10mg/kg BW) and sodium arsenite (Sa; 10mg/kg BW), respectively. Results showed that activities of antioxidant enzymes decreased significantly due to oxidative stress generated by sodium arsenite. Lipid peroxidation also increased due to sodium arsenite administration. alpha-Eleostearic acid and punicic acid acted as antioxidant and caused mostly all the altered parameters restored to normal level. Results also showed that antioxidant activity of alpha-eleostearic acid was more predominant than that of punicic acid.

Topics: Administration, Oral; Animal Feed; Animals; Antioxidants; Arsenites; Brain; Catalase; Linolenic Acids; Lipid Peroxidation; Lipids; Liver; Male; Oxidative Stress; Rats; Rats, Inbred Strains; Sodium Compounds; Superoxide Dismutase