elastin has been researched along with uranyl-acetate* in 2 studies
2 other study(ies) available for elastin and uranyl-acetate
| Article | Year |
|---|---|
Ultrastructural visualization of elastic fibres with a tannate--metal salt method.
A modification of the tannic acid-metal salt method was applied as an ultrastructural stain for elastin. Thin sections of glutaraldehyde-fixed, embedded rat aorta and rabbit elastic cartilage, with and without osmication, were examined. Raising the pH of the tannic acid solution from 2.7 to 9.0 progressively increased the electron-density of elastic fibres and collagen fibrils in osmicated and unosmicated specimens. The maximum tannic acid staining of elastic fibres was observed in the pH range 7.0-9.0. Collagen staining, although less intense than that of elastic fibres, was also greatest in this pH range. Elastic fibres in osmicated specimens demonstrated the strongest tannic acid staining with a minimal increase in density of collagen and cell nuclei when compared to the unosmicated specimens. Sequential treatments of osmicated specimens with tannic acid pH 7.0-9.0, and uranyl acetate, pH 4.1, enhanced the density of the elastin intensely, increased collagen staining moderately, but hardly increased the density of nuclei and microfibrils. In elastase-digested osmicated specimens, all tannic acid (pH 7.0)-uranyl acetate-reactive elastin was selectively removed. These results demonstrate that all the neutral and alkaline tannic acid-uranyl acetate methods can be used as a postembedment stain for elastin specimens fixed in glutaraldehyde and osmium tetroxide. Topics: Animals; Aorta; Ear, External; Elastic Tissue; Elastin; Fixatives; Hydrolyzable Tannins; Organometallic Compounds; Rabbits; Rats; Rats, Inbred Strains; Staining and Labeling; Tannins; Uranium | 1985 |
A routine method for contrasting elastin at the ultrastructural level.
A reliable, simple, and inexpensive method for ultrastructural investigation of elastin is described. This method uses uranyl acetate dissolved in absolute methanol, followed by an optional lead citrate counterstain. The procedure was tested on a number of animal and human tissues that had been fixed and processed differently. Topics: Animals; Aorta; Cartilage; Cats; Elastin; Gingiva; Guinea Pigs; Humans; Liver; Lung; Mesentery; Mice; Microscopy, Electron; Organometallic Compounds; Rats; Skin; Staining and Labeling; Uranium | 1984 |