elastin and maleimide

elastin has been researched along with maleimide* in 3 studies

Other Studies

3 other study(ies) available for elastin and maleimide

ArticleYear
Maleimide-Functionalized Poly(2-Oxazoline)s and Their Conjugation to Elastin-Like Polypeptides.
    Macromolecular bioscience, 2016, Volume: 16, Issue:3

    The design of drug delivery systems capable of efficiently delivering poorly soluble drugs to target sites still remains a major challenge. Such materials require several different functionalities; typically, these materials should be biodegradable and nontoxic, nonimmunogenic, responsive to their environment, and soluble in aqueous solution while retaining the ability to solubilize hydrophobic drugs. Here, a polypeptide-polymer hybrid of elastin-like polypeptides (ELPs) and poly(2-oxazoline)s (POx) is reported. This paper describes the chemical synthesis, physical characteristics, and drug loading potential of these novel hybrid macromolecules. A novel method is introduced for terminal functionalization of POx with protected maleimide moieties. Following recovery of the maleimide group via a retro Diels-Alder reaction, the consecutive Michael addition of thiol-functionalized ELPs yields the desired protein-polymer conjugate. These conjugates form nanoparticles in aqueous solution capable of solubilizing the anti-cancer drug paclitaxel with up to 8 wt% loading.

    Topics: Elastin; Maleimides; Oxazoles; Recombinant Proteins

2016
Maleimide-thiol coupling of a bioactive peptide to an elastin-like protein polymer.
    Acta biomaterialia, 2012, Volume: 8, Issue:2

    Recombinant elastin-like protein (ELP) polymers display several favorable characteristics for tissue repair and replacement as well as drug delivery applications. However, these materials are derived from peptide sequences that do not lend themselves to cell adhesion, migration, or proliferation. This report describes the chemoselective ligation of peptide linkers bearing the bioactive RGD sequence to the surface of ELP hydrogels. Initially, cystamine is conjugated to ELP, followed by the temperature-driven formation of elastomeric ELP hydrogels. Cystamine reduction produces reactive thiols that are coupled to the RGD peptide linker via a terminal maleimide group. Investigations into the behavior of endothelial cells and mesenchymal stem cells on the RGD-modified ELP hydrogel surface reveal significantly enhanced attachment, spreading, migration and proliferation. Attached endothelial cells display a quiescent phenotype.

    Topics: Amino Acid Sequence; Animals; Biocompatible Materials; Cell Adhesion; Cell Movement; Cell Proliferation; Cells, Cultured; Elastin; Human Umbilical Vein Endothelial Cells; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate; Maleimides; Mesenchymal Stem Cells; Microscopy, Confocal; Molecular Sequence Data; Oligopeptides; Peptides; Sulfhydryl Compounds; Sus scrofa

2012
Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors.
    Journal of controlled release : official journal of the Controlled Release Society, 2006, Jan-10, Volume: 110, Issue:2

    A thermoresponsive, genetically engineered, elastin-like polypeptide (ELP) containing a C-terminal cysteine residue was synthesized and purified by inverse transition cycling (ITC) and conjugated to doxorubicin (Dox) molecules through four different pH-sensitive, maleimide-activated, hydrazone linkers. The efficiency of Dox activation, conjugation ratios to ELP and biophysical characterization-hydrodynamic radius (Rh) and the temperature transition kinetics-of the ELP-Dox conjugates and pH-mediated release of Dox were quantified in this study. Conjugation ratios of the maleimide-activated Dox to the thiol group of a unique cysteine in the ELP were close to unity. The Rh of the conjugate increased as the linker length between the ELP backbone and Dox was increased. The linker structure and length had little effect on the Tt of the ELP-Dox conjugates, as all conjugates exhibited Tt's that were similar to the native ELP. However, the ELP-Dox conjugates with longer linkers exhibited slower transition kinetics compared to the ELP-Dox conjugates with shorter linkers. The highest release of the ELP-Dox conjugate by cleavage of the hydrazone bond at pH 4 was nearly 80% over 72 h and was exhibited by the conjugate with the shortest linker.

    Topics: Antibiotics, Antineoplastic; Chemical Phenomena; Chemistry, Physical; Chromatography, High Pressure Liquid; Doxorubicin; Drug Delivery Systems; Elastin; Escherichia coli; Hydrazones; Hydrogen-Ion Concentration; Light; Maleimides; Neoplasms; Peptides; Protein Engineering; Scattering, Radiation; Spectrophotometry, Ultraviolet

2006