elastin and ferric-chloride

The lifetime of bioprosthetic heart valves (BHVs) is limited by the mechanical damage and calcification. The major components of BHVs are collagen and elastin. Collagen could be well protected by glutaraldehyde (GLUT) crosslinking, while elastin is not stabilized and has a high risk of degradation, which could lead to the calcification of BHVs. We aimed to develop methods for stabilizing elastin and decreasing calcification. We investigated the combined tannic acid (TA) or epigallocatechin gallate (EGCG) with ferric chloride to stabilize elastin and prevent calcification. We found that the amount of TA/EGCG bound to elastin was in a time-dependent pattern and this reaction showed better efficiency in acidic condition and ethanol-water mixed solvents. Moreover, Fe

Topics: Animals; Bioprosthesis; Calcinosis; Catechin; Chlorides; Collagen; Cross-Linking Reagents; Elastin; Ferric Compounds; Glutaral; Heart Valve Prosthesis; Male; Pericardium; Polyphenols; Protein Stability; Rats, Sprague-Dawley; Swine; Tannins; Tensile Strength