elastin has been researched along with 2-mercaptoacetate* in 1 studies
1 other study(ies) available for elastin and 2-mercaptoacetate
Article | Year |
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Co-operation between plasmin and elastase in elastin degradation by intact murine macrophages.
Intact, thioglycollate-stimulated murine macrophages cultured on an insoluble [3H]-elastin substratum progressively hydrolysed the elastin. Cell lysates had little activity. We compared the effect of various proteinase inhibitors on elastinolysis by either live cells or cell-free, elastase-rich conditioned medium. Only known inhibitors of macrophage elastase blocked the activity of elastase-rich cell-conditioned medium whereas inhibitors of cathepsin B also suppressed intact cell activity. Serum proteinase inhibitors blocked cell-derived soluble elastase activity but not intact cell elastolytic activity. We also observed that plasminogen added to the cell cultures markedly increased elastinolysis by live macrophages or cell-free elastase-rich medium. Purified plasmin alone had no measurable effect on native elastin. Additional experiments indicated that the plasmin enhancement was due to elastin-dependent activation of latent macrophage elastase. These results indicate that live macrophage elastinolysis is a co-operative process involving multiple proteinases, especially a cysteine proteinase(s) and elastase. Plasmin may be a physiological activator of latent macrophage elastase. Topics: Animals; Cells, Cultured; Elastin; Enzyme Activation; Female; Fibrinolysin; Macrophages; Mice; Mice, Inbred C3H; Pancreatic Elastase; Plasminogen; Protease Inhibitors; Thioglycolates | 1984 |