dolastatin-10 and 5-5--bis(8-(phenylamino)-1-naphthalenesulfonate)

Dolastatin 10 is an unusual peptide of marine origin which binds to tubulin in the vinblastine/maytansine/phomopsin-binding region and potently inhibits mitosis. Using N,N'-ethylenebis(iodoacetamide) (EBI) and iodo[14C]acetamide as probes for the effects of ligands on the thiol groups of tubulin, we found that dolastatin 10 has effects on the sulfhydryls indistinguishable from those of phomopsin A but quite different from those of vinblastine and maytansine. Using the binding of bis-5,5'-[8-(N-phenyl)aminonaphthalene-1-sulfonic acid] (BisANS) as a measure of tubulin decay, we found that dolastatin 10 resembled phomopsin A in that decay was not detectable by this assay in its presence. Interestingly, both otherwise very different peptides are among the most effective inhibitors of tubulin decay yet discovered.

Topics: Alkylation; Anilino Naphthalenesulfonates; Animals; Antineoplastic Agents; Brain Chemistry; Cattle; Cross-Linking Reagents; Cystine; Depsipeptides; Ethylenediamines; Microtubules; Mycotoxins; Oligopeptides; Protein Conformation; Sulfhydryl Compounds; Tubulin; Vinblastine