dmp-728 and potassium-phosphate

A specific and sensitive HPLC assay for the determination of DMP 728 in dog and rat plasma has been developed. The method involves solid-phase extraction of DMP 728 and the internal standard from plasma using a C2 column. The extracted compounds are derivatized with benzoin under alkaline conditions. Using a mixture of acetonitrile and 0.1 M potassium phosphate buffer (25:75, v/v, pH 7.4) as mobile phase, the derivatized products are separated on a Regis semipermeable surface C8 column and monitored fluorometrically using 325 nm and 425 nm as excitation and emission wavelengths, respectively. The assay is linear from 2.5 to 1000 ng/ml in dog plasma and from 5 to 1000 ng/ml in rat plasma. The limit of quantitation is 2.5 ng/ml using 0.5 ml of dog plasma and 5 ng/ml using 0.5 ml of rat plasma. The assay has been used in pharmacokinetic studies of DMP 728 in dogs and rats.

Topics: Acetonitriles; Animals; Benzoin; Chromatography, High Pressure Liquid; Dogs; Female; Hydrogen-Ion Concentration; Hydroxides; Mesylates; Peptides, Cyclic; Phosphates; Platelet Aggregation Inhibitors; Platelet Membrane Glycoproteins; Potassium Compounds; Rats; Reproducibility of Results; Sensitivity and Specificity; Temperature; Time Factors