dithiothreitol has been researched along with guanidine in 55 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 8 (14.55) | 18.7374 |
| 1990's | 31 (56.36) | 18.2507 |
| 2000's | 14 (25.45) | 29.6817 |
| 2010's | 1 (1.82) | 24.3611 |
| 2020's | 1 (1.82) | 2.80 |
| Authors | Studies |
|---|---|
| Mannik, M; Trujillo, PE | 1 |
| Fountoulakis, M | 1 |
| Bouvet, JP; Iscaki, S; Pillot, J; Pires, R; Quan, C | 1 |
| Clapp, C; Ferrara, N; Weiner, R | 1 |
| Kelly, SM; Price, NC | 1 |
| Akita, M; Matsuyama, S; Mizushima, S; Shinkai, A | 1 |
| Wallis, M; Wallis, OC | 1 |
| Casey, AD; Clamp, JR; Corfield, AP; Paraskeva, C | 1 |
| Amir, D; Haas, E | 1 |
| Holmgren, A; Slaby, I | 1 |
| Hamaguchi, K; Kawata, Y; Tanaka, S; Wada, K | 1 |
| Horowitz, PM; Tandon, S | 1 |
| Bekesi, E; Kung, HF; Manne, V | 1 |
| Deutsch, HF; Han, KK; Xu, LX; Zhang, A; Zhu, DX; Zhu, NC | 1 |
| Fickenscher, K; Scheibe, R | 1 |
| Snyder, GH | 1 |
| Haas, E; Ittah, V | 1 |
| Hellekant, G; Markley, JL; Ming, D | 1 |
| Dirr, H; Sluis-Cremer, N | 1 |
| Ramasarma, PR; Rao, AG; Rao, DR | 1 |
| McClelland, DA; Price, NC; Stevens, L | 1 |
| Goodarzi, G; Grandgenett, DP | 1 |
| Cesareni, G; Flossdorf, J; Hinz, HJ; Kokkinidis, M; Steif, C; Weber, P | 1 |
| Damaschun, G; Gast, K; Müller-Frohne, M; Nöppert, A; Zirwer, D | 1 |
| Adiga, PR; Karande, AA; Velu, NK | 1 |
| Bernard, AR; Goffin, L; Payton, MA; Proudfoot, AE; Wells, TN | 1 |
| Haniu, M; Hui, JO; Katta, V; Le, J; Rohde, MF; Rosenfeld, R | 1 |
| Kreimer, DI; Shin, I; Shnyrov, VL; Silman, I; Villar, E; Weiner, L | 1 |
| Hayashi, T; Muraoka, M; Nakazato, K | 1 |
| Gellman, SH; Rozema, D | 1 |
| Chang, JY | 1 |
| Wang, C; Yao, Y; Zhou, Y | 1 |
| Chen, Y; Xu, G | 1 |
| Burstein, Y; Peretz, M; Weiner, LM | 1 |
| Churchich, JE; Li, W | 1 |
| Chaffotte, AF; Delepierre, M; Goldberg, ME; Roux, P | 1 |
| Englander, SW; Qi, PX; Sosnick, TR | 1 |
| Bera, AK; Bernhardt, R | 1 |
| Hallén, D; Suurkuusk, M | 1 |
| Campoy, FJ; Fini, C; Flores-Flores, C; Martínez-Martínez, A; Muñoz-Delgado, E; Rodríguez-López, JN; Vidal, CJ | 1 |
| Cigić, B; Dahl, SW; Pain, RH | 1 |
| Herbst, R; Hoeger, D; Jaenicke, R; Kretschmar, M; Lubsen, NH; Wenk, M | 1 |
| Haas, E; Ittah, V; Landsman, P; Navon, A; Scheraga, HA | 1 |
| Cao, ZF; Park, YD; Zhou, HM | 1 |
| Ramakrishna, T; Raman, B; Rao, ChM; Rao, KS; Srinivas, V | 1 |
| Liu, Y; Ou, WB; Xie, Q; Yan, YB; Zhao, TJ; Zhou, HM | 1 |
| Han, W; Shi, J; Wang, Z; Xue, X; Yan, Z; Zhang, Y | 1 |
| Chang, W; Ding, X; Guo, Z; Mi, J; Zhao, M | 1 |
| Chang, JY; Lin, CC; Lu, BY | 1 |
| Hong, Y; Li, Z; Liu, B; Ni, J; Shen, Y; Sheng, D | 1 |
| Meng, FG; Mu, H; Park, YD; Yang, JM; Zhou, SM | 1 |
| Wingfield, PT | 1 |
| Kumar, A; Singh, S | 1 |
| Eger, BT; Kawaguchi, Y; Matsumura, T; Nishino, T; Okamoto, K; Pai, EF | 1 |
| Binhi, VN; Gudkov, SV; Matveeva, TA; Penkov, NV; Sarimov, RM | 1 |
55 other study(ies) available for dithiothreitol and guanidine
| Article | Year |
|---|---|
IgG is bound by antigen-antibody bonds and some IgG and albumin are bound by intermolecular disulfide bonds to cartilage in rheumatoid arthritis and osteoarthritis.
Topics: Antigen-Antibody Reactions; Arthritis, Rheumatoid; Buffers; Cartilage, Articular; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fluorescent Antibody Technique; Guanidine; Guanidines; Humans; Immunoglobulin G; Microbial Collagenase; Osteoarthritis; Serum Albumin | 1992 |
Unfolding intermediates of the extracellular domain of the interferon gamma receptor.
Topics: Blotting, Western; Catalysis; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanidines; Humans; Interferons; NADP; Oxidation-Reduction; Protein Conformation; Receptors, Immunologic; Receptors, Interferon; Thioredoxin-Disulfide Reductase; Thioredoxins; Urea | 1992 |
Non-immune VH-binding specificity of human protein Fv.
Topics: Chromatography, High Pressure Liquid; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Epitopes; Guanidine; Guanidines; Hepatitis C; Humans; Immunoglobulin Heavy Chains; Immunoglobulin Light Chains; Immunoglobulins; Lymphokines; Sialoglycoproteins | 1991 |
The 16K fragment of prolactin specifically inhibits basal or fibroblast growth factor stimulated growth of capillary endothelial cells.
Topics: Adrenal Cortex; Animals; Bicarbonates; Capillaries; Cattle; Cell Division; Cells, Cultured; Cerebral Cortex; Disulfides; Dithiothreitol; Endothelium, Vascular; Female; Fibroblast Growth Factors; Guanidine; Guanidines; Molecular Weight; Peptide Fragments; Prolactin; Rats | 1991 |
The unfolding and refolding of pig heart fumarase.
Topics: Animals; Circular Dichroism; Dialysis; Dithiothreitol; Fumarate Hydratase; Guanidine; Guanidines; Kinetics; Protein Conformation; Protein Denaturation; Swine | 1991 |
Quantitative renaturation from a guanidine-denatured state of the SecA dimer, a 200 KDa protein involved in protein secretion in Escherichia coli.
Topics: Adenosine Triphosphatases; Bacterial Proteins; Biological Transport; Circular Dichroism; Cross-Linking Reagents; Dithiothreitol; Escherichia coli; Escherichia coli Proteins; Guanidine; Guanidines; Membrane Transport Proteins; Protein Conformation; Protein Denaturation; SEC Translocation Channels; SecA Proteins; Structure-Activity Relationship | 1990 |
Purification and properties of a recombinant DNA-derived ovine growth hormone analogue (oGH1) expressed in Escherichia coli.
Topics: Amino Acid Sequence; Animals; Cell Fractionation; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Expression; Growth Hormone; Guanidine; Guanidines; Isoelectric Focusing; Isoelectric Point; Microscopy, Electron; Molecular Sequence Data; Plasmids; Recombinant Proteins; Sheep | 1990 |
Characterization of a sialic-acid-rich mucus glycoprotein secreted by a premalignant human colorectal adenoma cell line.
Topics: Adenoma; Colorectal Neoplasms; Dithiothreitol; Guanidine; Guanidines; Humans; Mucins; Mucus; Neoplasm Proteins; Precancerous Conditions; Sialic Acids; Tumor Cells, Cultured | 1990 |
Reduced bovine pancreatic trypsin inhibitor has a compact structure.
Topics: Aprotinin; Disulfides; Dithiothreitol; Edetic Acid; Energy Transfer; Guanidine; Guanidines; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Trypsin Inhibitors | 1988 |
Thioredoxin reductase-dependent insulin disulfide reduction by phage T7 DNA polymerase reflects dissociation of the enzyme into subunits.
Topics: Dithiothreitol; DNA-Directed DNA Polymerase; Escherichia coli; Guanidine; Guanidines; Insulin; Iodoacetates; Iodoacetic Acid; Kinetics; Macromolecular Substances; NADH, NADPH Oxidoreductases; T-Phages; Thioredoxin-Disulfide Reductase | 1989 |
Extrinsic 33-kilodalton protein of spinach oxygen-evolving complexes: kinetic studies of folding and disulfide reduction.
Topics: Chemical Phenomena; Chemistry; Chlorophyll; Chloroplasts; Disulfides; Dithiothreitol; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Light-Harvesting Protein Complexes; Molecular Weight; Oxidation-Reduction; Photosynthetic Reaction Center Complex Proteins; Photosystem II Protein Complex; Plant Proteins; Protein Conformation; Temperature; Thermodynamics | 1989 |
Reversible folding of rhodanese. Presence of intermediate(s) at equilibrium.
Topics: Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Nephelometry and Turbidimetry; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Sulfurtransferases; Thermodynamics; Thiosulfate Sulfurtransferase | 1989 |
Ha-ras proteins exhibit GTPase activity: point mutations that activate Ha-ras gene products result in decreased GTPase activity.
Topics: Amino Acid Sequence; Bacterial Proteins; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; GTP Phosphohydrolases; Guanidine; Guanidines; Guanosine Diphosphate; Harvey murine sarcoma virus; Magnesium; Mutation; Phosphoric Monoester Hydrolases; Sarcoma Viruses, Murine; Time Factors | 1985 |
Investigations of primary and secondary structure of porcine ubiquitin. Its N epsilon-acetylated lysine derivative.
Topics: Acetylation; Amino Acid Sequence; Animals; Cattle; Circular Dichroism; Dithiothreitol; Erythrocytes; Guanidine; Guanidines; High Mobility Group Proteins; Lysine; Male; Protein Conformation; Swine; Ubiquitins; Urea | 1986 |
Limited proteolysis of inactive tetrameric chloroplast NADP-malate dehydrogenase produces active dimers.
Topics: Amino Acids; Carboxypeptidases; Catalysis; Chloroplasts; Chromatography, Gel; Chromatography, High Pressure Liquid; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Fabaceae; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Malate Dehydrogenase; Molecular Weight; Oxaloacetates; Oxidation-Reduction; Peptide Fragments; Plants, Medicinal; Protein Denaturation | 1988 |
Intramolecular disulfide loop formation in a peptide containing two cysteines.
Topics: Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Glutathione; Guanidine; Guanidines; Iodoacetates; Kinetics; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Trypsin Inhibitor, Kunitz Soybean; Trypsin Inhibitors; Urea | 1987 |
Nonlocal interactions stabilize long range loops in the initial folding intermediates of reduced bovine pancreatic trypsin inhibitor.
Topics: Aprotinin; Dithiothreitol; Drug Stability; Energy Transfer; Guanidine; Guanidines; Hydrogen-Ion Concentration; Models, Molecular; Oxidation-Reduction; Protein Conformation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Temperature | 1995 |
Quantification of cysteinyl sulfhydryl residues in peptides and proteins by ESI-MS or MALDI-MS.
Topics: Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Guanidine; Guanidines; Indicators and Reagents; Mass Spectrometry; Peptides; Plant Proteins; Plants; Pyridines; Sulfhydryl Compounds | 1995 |
Conformational stability of Cys45-alkylated and hydrogen peroxide-oxidised glutathione S-transferase.
Topics: Alkylation; Animals; Cysteine; Dithiothreitol; Enzyme Stability; Glutathione; Glutathione Transferase; Guanidine; Guanidines; Hydrogen Peroxide; Naphthalenesulfonates; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Structure-Activity Relationship; Swine; Thermodynamics | 1995 |
Role of disulfide linkages in structure and activity of proteinase inhibitor from horsegram (Dolichos biflorus).
Topics: Disulfides; Dithiothreitol; Fabaceae; Guanidine; Guanidines; Oxidation-Reduction; Plants, Medicinal; Protease Inhibitors; Protein Folding; Urea | 1995 |
The refolding of hen egg white riboflavin binding protein.
Topics: Animals; Carrier Proteins; Chickens; Dithiothreitol; Edetic Acid; Female; Guanidine; Guanidines; Membrane Transport Proteins; Phosphorylation; Protein Conformation; Protein Denaturation; Protein Folding; Riboflavin; Spectrometry, Fluorescence; Tryptophan; Urea | 1995 |
Folding of the multidomain human immunodeficiency virus type-I integrase.
Topics: Ammonium Sulfate; Blotting, Western; Chemical Precipitation; Chromatography, Gel; Dithiothreitol; DNA Nucleotidyltransferases; DNA, Viral; Enzyme Stability; Freezing; Guanidine; Guanidines; HIV Long Terminal Repeat; HIV-1; Hot Temperature; Integrases; Protein Denaturation; Protein Folding; Recombinant Proteins | 1994 |
Subunit interactions provide a significant contribution to the stability of the dimeric four-alpha-helical-bundle protein ROP.
Topics: Bacterial Proteins; Calorimetry, Differential Scanning; Circular Dichroism; Colicins; Dithiothreitol; Drug Stability; Guanidine; Guanidines; Macromolecular Substances; Mathematics; Models, Molecular; Protein Denaturation; Protein Structure, Secondary; RNA-Binding Proteins; Spectrophotometry, Ultraviolet; Thermodynamics | 1993 |
Reduced-denatured ribonuclease A is not in a compact state.
Topics: Animals; Cattle; Circular Dichroism; Dithiothreitol; Guanidine; Guanidines; Light; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Ribonuclease, Pancreatic; Scattering, Radiation | 1996 |
Refolding of riboflavin carrier protein as probed by biochemical and immunological parameters.
Topics: Animals; Antibodies, Monoclonal; Carrier Proteins; Chickens; Chromatography, Gel; Disulfides; Dithiothreitol; Egg White; Epitopes; Guanidine; Guanidines; Membrane Transport Proteins; Oxidation-Reduction; Protein Binding; Protein Denaturation; Protein Folding; Riboflavin; Spectrometry, Fluorescence | 1996 |
In vivo and in vitro folding of a recombinant metalloenzyme, phosphomannose isomerase.
Topics: Candida albicans; Chromatography, Gel; Cloning, Molecular; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Guanidines; Kinetics; Mannose-6-Phosphate Isomerase; Metalloproteins; Molecular Chaperones; Protein Denaturation; Protein Folding; Recombinant Proteins; Zinc | 1996 |
Human neurotrophin-3: a one-step peptide mapping method and complete disulfide characterization of the recombinant protein.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Chymotrypsin; Cysteine; Disulfides; Dithiothreitol; Escherichia coli; Guanidine; Guanidines; Humans; Molecular Sequence Data; Nerve Growth Factors; Neurotrophin 3; Peptide Fragments; Peptide Mapping; Protein Denaturation; Recombinant Proteins; Thermolysin | 1996 |
Two partially unfolded states of Torpedo californica acetylcholinesterase.
Topics: Acetylcholinesterase; Animals; Calorimetry, Differential Scanning; Chemical Phenomena; Chemistry, Physical; Cholinesterase Inhibitors; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electric Organ; Electron Spin Resonance Spectroscopy; Guanidine; Guanidines; Macromolecular Substances; Protein Folding; Spectrometry, Fluorescence; Sulfhydryl Reagents; Torpedo | 1996 |
Gel formation from the type IV collagen isolated from bovine lens capsule in guanidine-HCl and dithiothreitol.
Topics: Animals; Buffers; Cattle; Circular Dichroism; Collagen; Dithiothreitol; Gels; Guanidine; Guanidines; Lens Capsule, Crystalline | 1996 |
Artificial chaperone-assisted refolding of denatured-reduced lysozyme: modulation of the competition between renaturation and aggregation.
Topics: Adamantane; Animals; Cetrimonium; Cetrimonium Compounds; Chickens; Circular Dichroism; Cyclodextrins; Detergents; Dithiothreitol; Egg White; Female; Fluorescence; Glutathione; Guanidine; Guanidines; Kinetics; Molecular Chaperones; Muramidase; Phenols; Protein Denaturation; Protein Folding | 1996 |
A two-stage mechanism for the reductive unfolding of disulfide-containing proteins.
Topics: Animals; Arthropod Proteins; Cattle; Disulfides; Dithiothreitol; Guanidine; Guanidines; Hirudins; Intercellular Signaling Peptides and Proteins; Mercaptoethanol; Oxidation-Reduction; Peptides; Protein Denaturation; Protein Folding; Ribonuclease, Pancreatic; Ticks | 1997 |
Both the isomerase and chaperone activities of protein disulfide isomerase are required for the reactivation of reduced and denatured acidic phospholipase A2.
Topics: Agkistrodon; Animals; Crotalid Venoms; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Glutathione; Guanidine; Guanidines; Isomerases; Kinetics; Methylation; Models, Chemical; Molecular Chaperones; Oxidation-Reduction; Phospholipases A; Phospholipases A2; Protein Denaturation; Protein Disulfide-Isomerases; Protein Folding; Scattering, Radiation | 1997 |
Activation of spinach chloroplast fructose-1,6-bisphosphatase by monovalent cations.
Topics: Ammonium Chloride; Cations, Monovalent; Chloroplasts; Dithiothreitol; Enzyme Activation; Fructose-Bisphosphatase; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Magnesium; Potassium; Spinacia oleracea | 1997 |
Cysteine reactivity in Thermoanaerobacter brockii alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Bacteria, Anaerobic; Cysteine; Disulfides; Dithiothreitol; Electron Spin Resonance Spectroscopy; Gram-Positive Asporogenous Rods, Irregular; Guanidine; Guanidines; Iodoacetates; Iodoacetic Acid; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Spin Labels; Thermodynamics | 1997 |
Activation of partially folded mitochondrial malate dehydrogenase by thioredoxin.
Topics: Anilino Naphthalenesulfonates; Animals; Chromatography, Gel; Circular Dichroism; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Malate Dehydrogenase; Mitochondria, Heart; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Swine; Thioredoxins | 1997 |
Kinetics of secondary structure recovery during the refolding of reduced hen egg white lysozyme.
Topics: Animals; Chickens; Circular Dichroism; Dithiothreitol; Enzyme Stability; Female; Glutathione; Glutathione Disulfide; Guanidine; Guanidines; Kinetics; Magnetic Resonance Spectroscopy; Models, Chemical; Muramidase; Oxidation-Reduction; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence | 1997 |
The burst phase in ribonuclease A folding and solvent dependence of the unfolded state.
Topics: Animals; Cattle; Circular Dichroism; Disulfides; Dithiothreitol; Guanidine; Kinetics; Protein Denaturation; Protein Folding; Ribonuclease, Pancreatic; Solvents; Sulfhydryl Reagents | 1998 |
GroEL-assisted and -unassisted refolding of mature and precursor adrenodoxin: the role of the precursor sequence.
Topics: Adenosine Triphosphate; Adrenodoxin; Animals; Cattle; Chaperonin 10; Chaperonin 60; Dithiothreitol; Electron Transport; Escherichia coli; Guanidine; Holoenzymes; Inclusion Bodies; Kinetics; Protein Binding; Protein Denaturation; Protein Folding; Protein Precursors; Recombinant Proteins; Solubility; Thermodynamics | 1999 |
Denaturation of apolipoprotein A-I and the monomer form of apolipoprotein A-I(Milano).
Topics: Apolipoprotein A-I; Calorimetry, Differential Scanning; Circular Dichroism; Dithiothreitol; Genetic Variation; Guanidine; Hot Temperature; Humans; Models, Chemical; Oxidation-Reduction; Protein Denaturation; Protein Structure, Quaternary; Ultraviolet Rays | 1999 |
The ecto-5'-nucleotidase subunits in dimers are not linked by disulfide bridges but by non-covalent bonds.
Topics: 5'-Nucleotidase; Animals; Cattle; Centrifugation, Density Gradient; Chromatography, High Pressure Liquid; Dimerization; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidine; Male; Protein Structure, Quaternary; Sulfhydryl Compounds; Urea | 2000 |
The residual pro-part of cathepsin C fulfills the criteria required for an intramolecular chaperone in folding and stabilizing the human proenzyme.
Topics: Cathepsin C; Chromatography, Gel; Circular Dichroism; Colorimetry; Dithiothreitol; Enzyme Precursors; Enzyme Stability; Guanidine; Humans; Kinetics; Molecular Chaperones; Oxidation-Reduction; Peptide Fragments; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Recombinant Proteins; Reducing Agents; Thermodynamics; Urea | 2000 |
Gamma S-crystallin of bovine and human eye lens: solution structure, stability and folding of the intact two-domain protein and its separate domains.
Topics: Animals; Calorimetry, Differential Scanning; Cattle; Circular Dichroism; Cloning, Molecular; Crystallins; Dithiothreitol; Escherichia coli; Guanidine; Humans; Hydrogen-Ion Concentration; Kinetics; Lens, Crystalline; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Tertiary; Recombinant Proteins; Spectrometry, Fluorescence; Temperature; Thermodynamics | 2000 |
Distributions of intramolecular distances in the reduced and denatured states of bovine pancreatic ribonuclease A. Folding initiation structures in the C-terminal portions of the reduced protein.
Topics: Animals; Cattle; Crystallography, X-Ray; Dithiothreitol; Energy Transfer; Fluorescence Polarization; Fluorescent Dyes; Guanidine; Mutagenesis, Site-Directed; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Reducing Agents; Ribonuclease, Pancreatic; Spectrometry, Fluorescence; Tryptophan | 2001 |
Reactivation kinetics of guanidine hydrochloride-denatured creatine kinase measured using the substrate reaction.
Topics: Creatine; Creatine Kinase; Dithiothreitol; Edetic Acid; Enzyme Activation; Guanidine; Kinetics; Magnesium; Protein Denaturation | 2001 |
Structural perturbation and enhancement of the chaperone-like activity of alpha-crystallin by arginine hydrochloride.
Topics: Animals; Arginine; Cattle; Centrifugation, Density Gradient; Circular Dichroism; Crystallins; Dithiothreitol; Guanidine; Insulin; Protein Conformation; Pyrenes; Solubility; Spectrometry, Fluorescence; Time Factors | 2003 |
Catalysis of creatine kinase refolding by protein disulfide isomerase involves disulfide cross-link and dimer to tetramer switch.
Topics: Animals; Creatine Kinase; Dithiothreitol; Guanidine; Humans; Isoenzymes; Models, Theoretical; Molecular Chaperones; Protein Denaturation; Protein Disulfide-Isomerases; Protein Folding; Protein Renaturation; Protein Structure, Quaternary; Rabbits | 2005 |
Production and purification of recombinant human BLyS mutant from inclusion bodies.
Topics: Amino Acid Sequence; B-Cell Activating Factor; Chromatography, Gel; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Epitopes, T-Lymphocyte; Escherichia coli; Gene Expression; Guanidine; Humans; Inclusion Bodies; Membrane Proteins; Molecular Sequence Data; Mutation; Protein Folding; Recombinant Fusion Proteins; Tetanus Toxoid; Tumor Necrosis Factor-alpha; Vaccines, Synthetic | 2005 |
Screening of carbohydrate-specific phage antibodies against recombinant human erythropoietin (rhuEPO) using a phage display antibody library: preliminary study.
Topics: Antibodies; Antibody Specificity; Carbohydrates; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Erythropoietin; Glycosylation; Guanidine; Humans; Immunoglobulin Fragments; In Vitro Techniques; Oxidation-Reduction; Peptide Library; Periodic Acid; Recombinant Proteins | 2006 |
Conformational stability of secretory leucocyte protease inhibitor: a protein with no hydrophobic core and very little secondary structure.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithiothreitol; Guanidine; Guanidines; Humans; Hydrophobic and Hydrophilic Interactions; Isothiocyanates; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteinase Inhibitory Proteins, Secretory; Proteins; Recombinant Proteins; Secretory Leukocyte Peptidase Inhibitor; Thermodynamics; Urea | 2006 |
Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii.
Topics: Cations, Divalent; Cloning, Molecular; Dimerization; Dimethyl Sulfoxide; Dithiothreitol; Enzyme Stability; Escherichia coli; Guanidine; Hexosaminidases; Hydrogen-Ion Concentration; Pyrococcus horikoshii; Recombinant Proteins; Sodium Dodecyl Sulfate; Temperature; Urea | 2006 |
Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathway.
Topics: Anilino Naphthalenesulfonates; Animals; Binding Sites; Chromatography, Gel; Circular Dichroism; Creatine Kinase; Cysteine; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; Fluorescent Dyes; Guanidine; Kinetics; Models, Biological; Muscles; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Rabbits; Spectrophotometry, Ultraviolet | 2007 |
Use of protein folding reagents.
Topics: Dithiothreitol; Glutathione; Guanidine; Mercaptoethanol; Oxidants; Protein Denaturation; Protein Folding; Reducing Agents; Sulfhydryl Reagents; Urea | 2001 |
Interaction of chaperone alpha-crystallin with unfolded state of alpha-amylase: Implications for reconstitution of the active enzyme.
Topics: alpha-Amylases; alpha-Crystallins; Animals; Cattle; Chromatography, Gel; Dithiothreitol; Guanidine; Light; Pancreas; Protein Denaturation; Salicylic Acid; Spectrometry, Fluorescence; Substrate Specificity; Swine; Time Factors | 2009 |
The C-terminal peptide plays a role in the formation of an intermediate form during the transition between xanthine dehydrogenase and xanthine oxidase.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Crystallography, X-Ray; Cysteine; Dithiothreitol; Guanidine; Liver; Models, Biological; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Protein Conformation; Rats; Recombinant Proteins; Sequence Deletion; Sequence Homology, Amino Acid; Static Electricity; Sulfhydryl Reagents; Xanthine Dehydrogenase; Xanthine Oxidase | 2015 |
Unfolding and Aggregation of Lysozyme under the Combined Action of Dithiothreitol and Guanidine Hydrochloride: Optical Studies.
Topics: Animals; Chickens; Dithiothreitol; Guanidine; Muramidase; Protein Aggregates; Protein Unfolding; Spectrophotometry, Ultraviolet | 2021 |