dithionitrobenzoic acid has been researched along with cysteine in 368 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 103 (27.99) | 18.7374 |
| 1990's | 149 (40.49) | 18.2507 |
| 2000's | 88 (23.91) | 29.6817 |
| 2010's | 24 (6.52) | 24.3611 |
| 2020's | 4 (1.09) | 2.80 |
| Authors | Studies |
|---|---|
| Laishley, EJ | 1 |
| Dunlap, RB; Lewis, CA; Munroe, WA | 1 |
| Blakeley, RL; Riddles, PW; Zerner, B | 1 |
| Iwakura, M; Katsuki, H; Tokushige, M | 1 |
| Fridovich, I; Malinowski, DP | 1 |
| Horigome, T; Yamashita, T | 1 |
| Leskovac, V; Pavkov-Pericin, D | 1 |
| Cain, RB; Johnston, JB; Murray, K | 1 |
| Sato, M; Sato, T; Sawada, M | 1 |
| Otieno, S | 1 |
| Hartmann, GR; Neudecker, TJ | 1 |
| Schonbeck, ND; Shafer, JA; Skalski, M | 1 |
| Boettcher, B; Martinez-Carrion, M | 1 |
| Bennett, CD; Williams, MN | 1 |
| Barnard, EA; Bhargava, AK; Otieno, S; Serelis, D | 1 |
| Higgins, H; Miles, EW | 1 |
| Goldberg, ME; Högberg-Raibaud, A | 1 |
| Bode, J | 1 |
| Jones, WM; Manning, JM; Soper, TS | 1 |
| Barra, D; Bossa, F; Doonan, S; Martini, F; Montarani, G | 1 |
| Claeyssens, M; de Bruyne, CK; Saman, E | 1 |
| Christen, P; Gehring, H | 1 |
| Kram, R; Schmitt, H | 1 |
| Carlson, GM; Colombo, G; Lardy, HA | 2 |
| Dombrádi, V; Vereb, G | 1 |
| Azocar, O; Muñoz, E | 1 |
| Feeney, RE; Nashef, AS; Osuga, DT | 1 |
| Wu, JY | 1 |
| Cannella, C; Cavallini, D; Costa, M; Pecci, L; Pensa, B | 1 |
| Madelian, V; Warren, WA | 1 |
| Baliga, BS; Munro, HN; Nelson, E | 1 |
| Cagan, RH; Morris, JA | 1 |
| Kochkina, VM; Torchinskii, YM | 1 |
| Crichton, RR; Wetz, K | 1 |
| Gee, N; Gore, MG; Greasley, PJ; Knowles, MR; McAllister, G; Ragan, CI | 1 |
| Anderson, R | 1 |
| Gee, NS; Gore, MG; Greasley, PJ; Knowles, MR; McAllister, G; Ragan, CI | 1 |
| Pleckaityte, M; Siksnys, V | 1 |
| Bauerle, R; Stephens, CM | 1 |
| Bower, SM; Chantler, PD; Wang, Y | 1 |
| Bekkali, M; Matveeva, LN; Mezentsev, AV | 1 |
| Brand, L; Wu, P | 1 |
| Oleinikov, A; Traut, RR; Zecherle, GN | 1 |
| Deyashiki, Y; Hara, A; Nakayama, T; Sawada, H; Shinoda, M; Tanabe, H | 1 |
| Engesser, L; Grimbergen, J; Haverkate, F; Kerst, AF; Koopman, J; Lord, ST; Nováková, I | 1 |
| Johnson, WC; Palczewski, K; Riazance-Lawrence, JH | 1 |
| Drewes, G; Faulstich, H; Heintz, D | 1 |
| Brown, PL; Mecham, L; Mecham, RP; Tisdale, C | 1 |
| Ahn, HS; Foster, C; Foster, M; Sybertz, E; Wells, JN | 1 |
| Ball, EH; Kilic, F | 1 |
| Fujimoto, H; Haneda, M; Nakatsuka, M; Nisimoto, Y; Nomura, S; Ohta, T; Shibata, Y; Takeuchi, F; Tamai, T; Tsubouchi, R | 1 |
| Charles, H; Simms, SA; Subbaramaiah, K | 1 |
| Cabelli, DE; Fong, NM; Gallegos, C; Getzoff, ED; Hallewell, RA; Imlay, KC; Lee, P; Mullenbach, GT; Tainer, JA; Tekamp-Olson, P | 1 |
| Atkinson, T; Gore, MG; Scawen, MD; Spencer, P | 1 |
| Hamilton, GA; Skorczynski, SS; Yang, CS | 1 |
| Khan, MF; Sorenson, JR | 1 |
| Cismowski, MJ; Huang, PC | 1 |
| Biber, TU; Ford, GD; Lyall, V; Suzuki, Y | 1 |
| Chang, SH; Chen, Z; Lee, WR; Lu, L; Shirley, M | 1 |
| Cyran, FP; Fisher, MT; Ginsburg, A; Han, MK; Kim, SH | 1 |
| Rao, US; Scarborough, GA | 1 |
| Angleton, EL; Birkedal-Hansen, H; Springman, EB; Van Wart, HE | 1 |
| Dong, Q; Fromm, HJ; Liu, F; Soans, C | 1 |
| Harlow, KW; Switzer, RL | 1 |
| Ikehara, M; Kanaya, S; Katsuda, C; Kimura, S | 1 |
| Drewes, G; Faulstich, H | 1 |
| Deshpande, V; Hinge, J; Rao, M | 1 |
| Green, BN; Smith, S; Witkowska, HE | 1 |
| Asano, S; Di Donato, A; Manning, JM; Martínez del Pozo, A; Masu, Y; Merola, M; Recsei, P; Tanaka, H; Tanizawa, K; Ueno, H | 1 |
| Bhatnagar, A; Das, B; Liu, SQ; Srivastava, SK | 1 |
| Benjouad, A; Desmadril, M; el Hachimi, Z; Tijane, M; Yon, JM | 1 |
| Beharry, S; Bragg, PD | 1 |
| Shewchuk, LM; Verdine, GL; Walsh, CT | 1 |
| Bonelli, FS; Jonas, A | 1 |
| Wilton, DC | 1 |
| Heese, D; Röhm, KH | 1 |
| Altamirano, MM; Calcagno, M; Mulliert, G | 1 |
| Brunn, SA; Ericson, MC | 1 |
| Hartman, FC; Omnaas, J; Porter, MA | 1 |
| Gill, GN; Monken, CE | 1 |
| Inano, H; Tamaoki, B | 1 |
| Fujise, N; Nishimura, M; Yagasaki, O | 1 |
| Kato, H; Kimura, A; Nishioka, T; Oda, J; Tanaka, T | 1 |
| Inano, H | 1 |
| Krishnaswamy, S; Mann, KG | 1 |
| Fujioka, M; Konishi, K; Takata, Y | 1 |
| Alberta, JA; Fisher, RR; Modrak, DE; Wu, LN | 1 |
| Aykent, S; Huynh, QK; Kishore, GM; Padgette, SR; Sammons, RD; Sikorski, JA | 1 |
| Gargouri, Y; Moreau, H; Pieroni, G; Verger, R | 1 |
| Brockway, RA; Nakatani, M; Paglia, DE; Toohey, JI; Valentine, WN | 1 |
| Daly, TJ; Matthews, KS; Olson, JS | 1 |
| Cornman, EW; Mottonen, J; Simms, SA; Stock, J | 1 |
| Joseph, D; Lehrer, SS | 1 |
| Lang, CA; Richie, JP | 1 |
| Adamo, AM; Aloise, PA; Oteiza, PI; Paladini, AA; Paladini, AC; Soto, EF | 1 |
| Fujioka, M; Konishi, K; Ogawa, H; Takata, Y | 1 |
| Ichikawa, Y; Tomita, S; Tsubaki, M; Tsuneoka, Y | 1 |
| Dolphin, PJ; Jauhiainen, M | 1 |
| Kumar, S; Lennane, J; Ratner, S | 1 |
| Katsume, T; Muratsubaki, H | 1 |
| Hong, PT; Tompa, P; Vas, M | 1 |
| King, MM | 1 |
| Black, SD; Coon, MJ | 1 |
| Składanowski, A; Zydowo, M | 1 |
| Levine, RL; Sliwkowski, MX | 1 |
| Doonan, S; Marra, E; O'Donovan, KM; Passarella, S; Quagliariello, E | 1 |
| Ahrné, L; Björck, L | 1 |
| Smith, S; Witkowski, A | 1 |
| Bergamini, CM; Ferrari, C; Poltronieri, L; Rippa, M; Signorini, M | 1 |
| Gilbert, HF; Izbicka-Dimitrijević, E | 1 |
| Gonias, SL; Pizzo, SV | 1 |
| Fabregat, I; Machado, A; Satrústegui, J | 1 |
| Bazaes, S; Beytía, E; Eyzaguirre, J; Gómez, I; Jabalquinto, AM; Solís de Ovando, F | 1 |
| Draijer, JW; Muijsers, AO; Van Gelder, BF; Verheul, FE | 1 |
| Matsuda, S; Minowa, O; Yagi, K | 1 |
| Hamaguchi, K; Horii, T; Kuramitsu, S; Ogawa, H; Ogawa, T; Tachibana, H | 1 |
| Davidson, BE; Hudson, GS; Wong, V | 1 |
| Angelaccio, S; Barra, D; Bossa, F; Doonan, S; Martini, F | 1 |
| Shur, SA; Skolysheva, LK; Vul'fson, PL | 1 |
| Franson, RC; Kyger, EM | 1 |
| Bull, P; Valenzuela, P; Wyneken, U | 1 |
| Mäntsälä, P; Myöhänen, T | 1 |
| Cennerazzo, MJ; Field, D; Karalis, AJ; Snyder, GH | 1 |
| Gracy, RW; Lu, HS | 1 |
| Candiano, G; Delfino, G; Ghiggeri, GM; Queirolo, C | 1 |
| Holmgren, A; Kallis, GB | 1 |
| Harper, ET; Wong, P | 1 |
| Laidler, PM; Taga, EM; Van Etten, RL | 1 |
| Inada, Y; Matsushima, A; Nishimura, H; Yokota, Y; Yoshida, K | 1 |
| Colman, RF; Johanson, RA | 2 |
| Hamaguchi, K; Kuramitsu, S; Ogawa, H; Ogawa, T | 1 |
| Gray, JC; Hooper, EA; Perham, RN | 1 |
| Massey, V; Olson, ST | 1 |
| Sano, S; Tsukamoto, I; Yoshinaga, T | 1 |
| Baker, HN; Dempsey, ME; Dimitriadou-Vafiadou, A; Howard, JB; Lorsbach, T; McCoy, KE | 1 |
| Dowd, SR; Ho, C; Pratt, EA; Sun, ZY | 1 |
| Hellekant, G; Markley, JL; Ming, D | 1 |
| Blättler, WA; Collinson, AR; Singh, R | 1 |
| Lei, Y; Liu, HW; Ploux, O; Vatanen, K | 1 |
| Han, G; Han, J; Han, P; Lawson, L | 1 |
| Engel, PC; Wang, XG | 1 |
| Dahm, LJ; Jones, DP | 1 |
| Christen, P; Gehring, H; Jakob, CA; Pan, P; Sandmeier, E | 1 |
| Jordan, PM; Spencer, P | 1 |
| Castelhano, AL; Copp, L; Feng, R; Krantz, A; Li, B; Stahl, M; Yuan, Z | 1 |
| Balasubramanian, KA; Nalini, S | 1 |
| D'Ettorre, C; Levine, RL | 1 |
| Belduz, AO; Glasgow, J; Harman, JG; Lee, EJ; Leu, SF | 1 |
| Gong, BJ; Park, HS; Tao, T | 1 |
| Gómez-Puyou, A; Pérez-Montfort, R; Zubillaga, RA | 1 |
| Fonda, ML; Gao, G | 1 |
| Horowitz, PM; Hua, S; Miller-Martini, DM | 1 |
| Frieden, C; Jiang, N | 1 |
| Lowey, S; Saraswat, LD; Wolff-Long, VL | 1 |
| Gailit, J | 1 |
| Evans, C; Thumser, AE; Wilton, DC; Worrall, AF | 1 |
| Giedroc, DP; Kodadek, T; Qiu, H | 1 |
| Karlström, AR; Levine, RL; Shames, BD | 1 |
| Cardemil, E; Encinas, MV; Kemp, RG; Latshaw, SP; Rojas, MC | 1 |
| Chen, LC; Nagase, H; Noelken, ME | 1 |
| Carlson, GM; Cassell, RG; Lewis, CT; Seyer, JM | 1 |
| Gomi, S; Imae, Y; Iwama, T; Lee, L | 1 |
| Egorov, AM; Egorova, OA; Galkin, AG; Marchenko, GN; Tishkov, VI; Tsygankov, IuD | 1 |
| Dementieva, LA; Egorov, AM; Egorova, OA; Galkin, AG; Kulakova, LB; Marchenko, GN; Sheluho, DV; Tishkov, VI | 1 |
| Bhakdi, S; Hilgert, K; Jursch, R; Kehoe, M; Palmer, M; Valeva, A; Weller, U | 1 |
| Evans, BR; Herrmann, PC; Tate, J; Woodward, J | 1 |
| Graceffa, P | 1 |
| Jurado, LA; Machín, I; Urbina, JA | 1 |
| Buechler, YJ; Bussell, SJ; Houston, LL; Parandoosh, Z; Ryder, M; Shen, C; Sosnowski, BA; Victor, KD | 1 |
| Hara, T; Kato, H; Nishioka, T; Oda, J; Tanaka, T | 1 |
| Bon, S; Massoulié, J; Rosenberry, TL; Wilson, EJ | 1 |
| Earm, YE; Han, J; Ho, WK; Kim, E | 1 |
| Davis, DA; Dorsey, K; Fales, HM; Kaufman, J; Levine, RL; Stahl, SJ; Wingfield, PT | 1 |
| Means, GE; Zhang, H | 1 |
| Gallagher, ME; Logan, CB; Mayhew, SG | 1 |
| Fillat, MF; Gallagher, ME; Malthouse, JP; Mayhew, SG | 1 |
| Gallagher, TM | 1 |
| Creighton, TE; Takahashi, N | 1 |
| Ramachandran, S; Udgaonkar, JB | 1 |
| Patel, MA; Salleh, HM; Woodard, RW | 1 |
| Colman, RW; Ghazaleh, FA; Omburo, GA | 1 |
| Kubo, K | 1 |
| Kanaani, J; Maltby, D; Somoza, JR; Wang, CC | 1 |
| Helmkamp, GM; Tremblay, JM; Voziyan, PA; Yarbrough, LR | 1 |
| Chu, Y; Lee, EY; Reimann, EM; Schlender, KK; Wilson, SE | 1 |
| Katiyar, SS; Sheikh, S | 1 |
| Huang, HW; Wang, KT | 1 |
| Herscovics, A; Lipari, F | 1 |
| Rabenstein, DL; Russell, J | 1 |
| Lamb, GD; Posterino, GS | 1 |
| Gabriel, JL; Pieringer, RA; Soprano, DR; Soprano, KJ; Wolfgang, CL; Zhang, Z | 1 |
| Board, PG; Gali, RR | 1 |
| Bedouelle, H; Blondel, A; Nageotte, R | 1 |
| Buè, MC; Ciriolo, MR; De Vito, P; Garaci, E; Incerpi, S; Lafavia, E; Palamara, AT; Rotilio, G | 1 |
| Ahmed, S; Kerrick, WG; Lin, X; Liu, W; Potter, JD; Putkey, JA; Zhang, M | 1 |
| Chong, S; Garcia, GA | 1 |
| Peter-Katalinić, J; Rizner, TL; Strupat, K; Zakelj-Mavric, M; Zorko, M | 1 |
| Heacock, DH; Liu, H; Musier-Forsyth, K; Stehlin, C | 1 |
| Björkbacka, H; Forsman, C; Johansson, IM; Skärfstad, E | 1 |
| Aitken, A; Learmonth, M | 2 |
| Maruyama, T; Narazaki, R; Otagiri, M | 1 |
| Emoto, T; Kojima, Y; Kurasaki, M; Oikawa, S; Okabe, M; Yamasaki, F | 1 |
| Agner, E; Engebretsen, M; Fischer, PM; Sandosham, J | 1 |
| Footer, M; Matsudaira, P; Sun, S | 1 |
| Díaz, M; Riquelme, G; Sepúlveda, FV | 1 |
| Pregel, MJ; Storer, AC | 1 |
| Bernal, JL; Manzanas, L; Marinero, P; Moral, O; Nozal, MJ; Rodriguez, E; Toribio, L | 1 |
| Abian, J; Caminade, E; Canosa, D; Carrascal, M; Daniel, R; Le Goffic, F; Martel, A | 1 |
| Ellis, HR; Poole, LB | 1 |
| Li Calzi, M; Poole, LB | 1 |
| Chen, ZH; Gopalakrishna, R; Gundimeda, U | 1 |
| Cho, HS; Choi, G; Choi, KY; Joo, S; Kim, SW; Oh, BH | 1 |
| Björnberg, O; Jensen, KF; Larsen, S; Rowland, P | 1 |
| Hediger, MA; Nussberger, S; Trotti, D; Volterra, A | 1 |
| Marrone, L; Viswanatha, T | 1 |
| Baines, IC; Kang, SW; Rhee, SG | 1 |
| Hamiche, A; Richard-Foy, H | 1 |
| Clonts, B; Mujumdar, VS; Parker, JL; Smiley, LM; Tyagi, SC | 1 |
| Deber, CM; Pomroy, NC | 1 |
| Antharavally, B; DasGupta, BR; Tepp, W | 1 |
| Cabrera, N; Garza-Ramos, G; Gómez-Puyou, A; Ostoa-Saloma, P; Pérez-Montfort, R; Saavedra-Lira, E; Tuena de Gómez-Puyou, M | 1 |
| Mulrooney, SB; Veine, DM; Wang, PF; Williams, CH | 1 |
| Bourgeois, S; Burlacu-Miron, S; Gilles, AM; Perrier, V; Surewicz, WK | 1 |
| Amiconi, G; Barra, D; Bellelli, A; Boumis, G; Canofeni, S; Di Simplicio, P; Lusini, L; Pascarella, S; Rossi, R | 1 |
| Ha, JH; Loh, SN | 1 |
| Jones, LR; Karim, CB; Karim, J; Stamm, JD; Thomas, DD | 1 |
| Bandyopadhyay, S; Deb, S; Roy, S | 1 |
| Rao, DN; Reddy, YV | 1 |
| Matsuzaki, R; Tanizawa, K | 1 |
| Lynch, JW | 1 |
| Bulygin, VV; Cross, RL; Duncan, TM | 1 |
| Fakler, B; Hahn, H; Ruppersberg, JP; Schulte, U; Wiesinger, H | 1 |
| Ghosh, SK; Pal, J | 1 |
| Jamal, J; Mandelman, D; Poulos, TL | 1 |
| Boussac, A; Burlacu-Miron, S; Gilles, AM; Meier, A; Perrier, V | 1 |
| Gioannini, TL; Hiller, JM; Onoprishvili, I; Simon, EJ | 1 |
| Decottignies, P; Goyer, A; Issakidis-Bourguet, E; Jacquot, JP; Lemaire, S; Miginiac-Maslow, M; Ruelland, E | 1 |
| Arscott, LD; Gilberger, TW; Müller, S; Wang, PF; Williams, CH | 1 |
| Alcántara, GH; de Gómez-Puyou, MT; Gao, XG; Garza-Ramos, G; Gómez-Puyou, A; Maldonado, E; Pérez-Montfort, R; Reyes-Vivas, H | 1 |
| Kozaki, A; Sasaki, Y | 1 |
| Bielicki, JK; Forte, TM | 1 |
| Barrett, WC; Chock, PB; DeGnore, JP; Fales, HM; Keng, YF; König, S; Yim, MB; Zhang, ZY | 1 |
| Brown, PR; Farnaud, S; Sohi, MK; Sutton, BJ; Tata, R; Wan, T | 1 |
| Huang, Y; Liu, M; Wang, E; Wang, Y; Wu, J | 1 |
| Engel, PC; Parker, A | 1 |
| Ohage, E; Steipe, B | 1 |
| Aizenman, E; Brimecombe, JC; Potthoff, WK | 1 |
| Rao, AG; Sudharshan, E | 1 |
| Dulhunty, AF; Fink, RH; Haarmann, CS | 1 |
| Prunell, A; Sivolob, A | 1 |
| Camici, G; Caselli, A; Degl'Innocenti, D; Manao, G; Marzocchini, R; Ramponi, G; Rosati, F | 1 |
| Ramachandran, S; Rami, BR; Udgaonkar, JB | 1 |
| Czerski, L; Sanders, CR | 1 |
| Kleinman, WA; Richie, JP | 1 |
| Basusarkar, P; Bhattacharyya, B; Bhattacharyya, S; Manna, T; Roy, S; Roychowdhury, M; Sarkar, N; Sarkar, T | 1 |
| Campoccia, G; Di Simplicio, P; Fanetti, G; Giannerini, F; Giustarini, D; Lusini, L; Rossi, R | 1 |
| Cordeiro, MN; Diniz, CR; Gomez, MV; Kalapothakis, E; Prado, MA; Reis, HJ; Romano-Silva, MA | 1 |
| Barre, A; Engelborghs, Y; Hao, Q; Peumans, WJ; Rougé, P; Sillen, A; Van Damme, EJ | 1 |
| Azza, S; Boschi-Muller, S; Branlant, G; Sanglier-Cianferani, S; Talfournier, F; Van Dorsselear, A | 1 |
| Eisaki, N; Matsuyama, A; Mino, K; Nakanishi, K; Sakiyama, T; Yamanoue, T | 1 |
| Dyllick-Brenzinger, M; Liu, M; Taylor, DE; Turner, RJ; Winstone, TL | 1 |
| Lee, SH; Wang, M; You, JS | 1 |
| Subbaiah, PV; Wang, K | 1 |
| Colombo, R; Dalle-Donne, I; Di Simplicio, P; Giannerini, F; Giustarini, D; Lusini, L; Milzani, A; Rossi, R | 1 |
| Diamond, DL; Hardy, SJ; Randall, LL; Topping, TB; Woodbury, RL | 1 |
| Engel, PC; Hayden, BM | 1 |
| Dulhunty, A; Green, D; Haarmann, C; Hart, J | 1 |
| Braun, PE; Gao, E; Gravel, M; Lee, J; O'Neill, RC | 1 |
| Blond, SY; Bolton, JL; Chang, M; Shin, YG; van Breemen, RB | 1 |
| Kunugi, S; Mitani, D; Tanaka, N | 1 |
| George, SP; Rao, MB | 1 |
| Buck, CJ; Mueller, EG; Palenchar, PM | 1 |
| Helmkamp, GM; Li, H; Tremblay, JM; Yarbrough, LR | 1 |
| Jevtovic-Todorovic, V; Mennerick, S; Meyenburg, A; Olney, JW; Perez-Reyes, E; Romano, C; Todorovic, SM; Zorumski, CF | 1 |
| Anthony, JR; Donohue, TJ; Newman, JD | 1 |
| Alam, SL; Butler, MC; Feng, Z; Loh, SN | 1 |
| Hwang, JY; Koch, KW; Schlesinger, R | 1 |
| McCormick, SJ; Tunnicliff, G | 1 |
| Sridevi, K; Udgaonkar, JB | 1 |
| Blume, A; Chen, H; Hinderlich, S; Reutter, W; Zimmermann-Kordmann, M | 1 |
| Dick, S; Frey, HE; Lepock, JR; Siemann, S; Viswanatha, T | 1 |
| Brown, LS; Lanyi, JK; Needleman, R | 1 |
| Nowak, T; Ríos, SE | 1 |
| Pitts, KE; Summers, AO | 1 |
| Bismuto, E; Febbraio, F; Likhtenshtein, GI; Lozinsky, E; Nucci, R; Shames, AI | 1 |
| Hwang, JY; Koch, KW | 1 |
| Gupta, K; Panda, D | 1 |
| Gao, Y; Huang, ZX; Yang, PY; Yu, WH; Zhang, SY | 1 |
| Chen, H; Du, W; He, S; Li, K; Liu, HW; Zhao, Z | 1 |
| Calamari, AS; Elefante, LC; Fornwald, JA; Johanson, KO; Jonak, ZL; Kane, J; Kirkpatrick, RB; Knecht, KA; Mao, J; Matico, RE; McDevitt, PJ; McLaughlin, MM; Moore, DD; Nwagwu, S; Patel, PS; Sathe, GM; Shatzman, AR; Tapley, PM; Trill, JJ; Trulli, SH; Yorke, AF | 1 |
| Bayley, H; Luchian, T; Shin, SH | 1 |
| Budge, PJ; Graham, BS; Lebowitz, J | 1 |
| Grazziani, S; Gutova, M; Hamann, M; Han, S; Makmura, L; Momand, J; Nguyen, J; Sheppard, S; Sun, XZ; Thomas, J; Tran, D; Vinci, C; Zhou, F | 1 |
| Colombo, R; Dalle-Donne, I; Giustarini, D; Milzani, A; Rossi, R | 1 |
| Mishra, S; Ruppel, N; VanHaaften, E; Ybe, JA | 1 |
| Blackburn, N; Okeley, NM; Paul, M; Stasser, JP; van der Donk, WA | 1 |
| Futatsugi, N; Hoseki, J; Kagamiyama, H; Konagaya, A; Kuramitsu, S; Okamoto, A; Suenaga, A; Taiji, M; Takada, N; Yano, T | 1 |
| Ballicora, MA; Preiss, J; Sivak, MN; Yep, A | 1 |
| Chang, CH; Chen, YJ; Huang, CY; Ko, CY; Liao, HK; Wu, TK | 1 |
| Gevaert, K; Ghesquière, B; Martens, L; Staes, A; Thomas, GR; Van Damme, J; Vandekerckhove, J | 1 |
| Cheng, Y; Hui, EF; Pan, JC; Zhou, HM | 1 |
| Ghosh, D; Nakajin, S; Ohno, S; Sugiyama, T | 1 |
| Jevtovic-Todorovic, V; Meyenburg, A; Todorovic, SM | 1 |
| Conway, ME; Hutson, SM; Poole, LB | 1 |
| An, JJ; Cho, SW; Choi, HS; Choi, SH; Choi, SY; Eum, WS; Jang, SH; Kang, TC; Kim, DW; Kim, SY; Kwon, OS; Lee, BR; Lee, KS; Park, J; Won, MH; Yoon, CS | 1 |
| Bai, G; Jin, Y; Liu, Z; Yang, W | 1 |
| Britto, PJ; Knipling, L; McPhie, P; Wolff, J | 1 |
| Arutyunov, D; Branlant, G; Marchal, S; Muronetz, V; Orlov, V; Rahuel-Clermont, S | 1 |
| Blache, D; Bourdon, E; Lagrost, L; Loreau, N | 1 |
| Bramey, T; De Groot, H; Kirsch, M; Pamp, K; Petrat, F | 1 |
| Hasegawa, G; Kikuchi, M; Kobayashi, Y; Saito, Y | 1 |
| Fujiwara, S; Imanaka, T; Nishikori, S; Shiraki, K; Takagi, M | 1 |
| Feng, Z; Qin, G; Xicheng, W | 1 |
| Fodeke, AA; Okonjo, KO | 1 |
| González-Segura, L; Mújica-Jiménez, C; Muñoz-Clares, RA; Velasco-García, R; Zaldívar-Machorro, VJ | 1 |
| Cormier, KS; Fox, JG; Rogers, AB | 1 |
| Basu, G; Chakrabarti, P; Saha, RP | 1 |
| Pezzementi, L; Rowland, M; Tsigelny, I; Wolfe, M | 1 |
| Caux-Thang, C; Duarte, V; Dupuy, J; El Ghazouani, A; Ferrer, JL; Ilango, S; Jacquamet, L; Latour, JM; Traoré, DA | 1 |
| Alizadeh, T; Cliff, MJ; Craven, CJ; Jelinska, C; Staniforth, RA; Waltho, JP | 1 |
| Hayashi, K; Inoue, K; Ishiguro, R; Kimura, M; Li, S; Maruyama, K; Sugisaki, H; Takashima, T | 1 |
| Helten, A; Koch, KW | 1 |
| Eckenroth, BE; Everse, SJ; Hondal, RJ; Rould, MA | 1 |
| Adeboye, O; Adediji, AT; Ezeh, CV; Fodeke, AA; Okonjo, KO | 1 |
| Ellis, HR; Jönsson, TJ; Poole, LB | 1 |
| He, HW; Li, J; Ma, Y; Shi, F; Zhao, TJ; Zhou, HM | 1 |
| Meng, FG; Mu, H; Park, YD; Yang, JM; Zhou, SM | 1 |
| Anish, R; Rao, M | 1 |
| Li, M; Wang, XY; Zhu, WJ | 1 |
| Babalola, JO; Bello, OS; Okonjo, KO | 1 |
| Carvajal, K; De la Mora-De la Mora, I; Enriquez-Flores, S; Gutierrez-Castrellon, P; Hernandez-Alcantara, G; Lopez-Velazquez, G; Reyes-Vivas, H; Rodriguez-Romero, A | 1 |
| Sivakami, S; Suji, G | 1 |
| Chen, HM; Chow, CY; Fang, HJ; Hu, CK; Tsong, TY; Wu, MC | 1 |
| Li, L; Mi, W; Su, XD | 1 |
| Lü, ZR; Park, YD; Wang, YJ; Zhang, YM; Zou, F; Zou, HC | 1 |
| Adeogun, I; Babalola, JO; Okonjo, KO | 1 |
| Herrmann, C; Kunzelmann, S; Vöpel, T | 1 |
| Kayastha, AM; Kumar, S | 1 |
| Beyl, S; Hering, S; Karmazinova, M; Klugbauer, N; Lacinova, L; Stary-Weinzinger, A; Suwattanasophon, C | 1 |
| Canals, A; Coll, M; Costa, M; Mantle, TJ; McManus, G | 1 |
| Fuchs, F; Lehrer, SS; Ly, S | 1 |
| Hynek, R; Kodíček, M; Krásný, L | 1 |
| Chung, HY; Jeong, HO; Li, C; Liu, XX; Park, D; Sun, S; Zhou, HM | 1 |
| Chibani, K; Gualberto, JM; Jacquot, JP; Rey, P; Rouhier, N; Tarrago, L; Wingsle, G | 1 |
| D'Ordine, RL; Davisson, VJ; Linger, RS; Thai, CJ | 1 |
| Enjabal, C; Jullian, M; Martinez, J; Maurras, A; Puget, K; Ronga, L; Sanchez, P; Subra, G; Verdié, P | 1 |
| Chen, K; Flynn, GC; Huang, G; Jiang, XG; Kaltenbrunner, O; Wang, T | 1 |
| Apak, R; Baki, S; Çelik, SE; Güçlü, K; Güngör, N; Özyürek, M | 1 |
| Blachowicz, L; Ghisi, V; Lau, AY; Plested, AJ; Roux, B; Salazar, H | 1 |
| Doughty, LM; Fellner, M; Jameson, GN; Wilbanks, SM | 1 |
| Koch, JR; Schmid, FX | 1 |
| Basak, S; Bera, K; Nag, M | 1 |
| Behnke, T; Falkenhagen, J; Hamers-Allin, C; Klein-Hartwig, K; Moser, M; Resch-Genger, U | 1 |
| Chai, Z; Chen, J; Hu, Y; Liu, R; Lu, H; Sun, H; Zhang, H; Zhang, J; Zhao, Y | 1 |
| Cserne Szappanos, H; Hool, L; Ingley, E; Muralidharan, P | 1 |
| Baiocco, P; Benni, I; Boffi, A; Bonamore, A; Calisti, L; Cardoso Trabuco, M; Falvo, E; Malatesta, F; Ruzicka, B | 1 |
| Furuki, K; Toyo'oka, T | 1 |
| Wang, W; Xu, J; Zhou, H | 1 |
| Hardy, JA; Hill, ME; Yildiz, M | 1 |
| Cho, HN; Jhee, KH; Kim, HJ; Kim, KR; Lee, JY; Park, SH; Yang, SA | 1 |
| Bocedi, A; Cattani, G; Cozzolino, F; Di Fusco, O; Gambardella, G; Pucci, P; Ricci, G; Ticconi, S | 1 |
| Ábrányi-Balogh, P; Keserű, GM; Mészáros, A; Nguyen, HH; Pauwels, K; Petri, L; Tompa, P; Vandenbussche, G | 1 |
| Albigès-Rizo, C; Boyault, C; Coute, Y; Destaing, O; Hiriart-Bryant, E; Knölker, HJ; Kraut, A; Martin, R; Oddou, C; Rivier, P; Valverde, MA; Vellino, S | 1 |
| Chernov'yants, MS; Popov, LD; Shcherbatykh, AA | 1 |
| Bae, WB; Jhee, KH; Kim, HJ | 1 |
1 review(s) available for dithionitrobenzoic acid and cysteine
| Article | Year |
|---|---|
How many cysteine residues regulate ryanodine receptor channel activity?
Topics: Animals; Calcium Signaling; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; Heart; Ion Transport; Muscle Contraction; Muscle Proteins; Muscle, Skeletal; Myocardial Contraction; Myocardium; Oxidants; Oxidation-Reduction; Protein Conformation; Protein Structure, Tertiary; Protein Subunits; Pyridines; Reducing Agents; Ryanodine Receptor Calcium Release Channel; Sarcoplasmic Reticulum; Structure-Activity Relationship; Sulfhydryl Compounds; Thimerosal | 2000 |
367 other study(ies) available for dithionitrobenzoic acid and cysteine
| Article | Year |
|---|---|
Regulation and properties of an invertase from Clostridium pasteurianum.
Topics: Cell-Free System; Clostridium; Cysteine; Dithionitrobenzoic Acid; Enzyme Induction; Enzyme Repression; Fructose; Glucose; Hot Temperature; Hydrogen-Ion Concentration; Hydroxymercuribenzoates; Phosphates; Sucrase; Sucrose | 1975 |
Kinetics of sulfhydryl group modification of thymidylate synthetase: a proposal for activation of catalytic cysteinly residues.
Topics: Binding Sites; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Kinetics; Lacticaseibacillus casei; Methyl Methanesulfonate; Methyltransferases; Thymidylate Synthase | 1978 |
Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
Topics: Absorption; Buffers; Chemical Phenomena; Chemistry; Color; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Hydrolysis; Nitrobenzoates; Salts; Spectrophotometry; Temperature | 1979 |
Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli W.
Topics: Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Ethylmaleimide; Kinetics; Magnesium; Malate Dehydrogenase; NADP; Spectrophotometry, Ultraviolet; Sulfhydryl Reagents; Thermodynamics | 1979 |
Subunit association and side-chain reactivities of bovine erythrocyte superoxide dismutase in denaturing solvents.
Topics: Animals; Cattle; Copper; Cysteine; Dithionitrobenzoic Acid; Edetic Acid; Erythrocytes; Guanidines; Macromolecular Substances; Molecular Weight; Phosphates; Protein Binding; Superoxide Dismutase; Urea; Zinc | 1979 |
The sulfhydryl groups involved in the active site of myosin B adenosinetriphosphatase. V. The possible localization of Sa and other sulfhydryl groups in myosin rods.
Topics: Adenosine Triphosphatases; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Kinetics; Molecular Weight; Muscles; Myosins; Protein Binding; Rabbits; Sulfhydryl Compounds | 1979 |
Evidence for a histidine and a cysteine residue in the substrate-binding site of yeast alcohol dehydrogenase.
Topics: Acetamides; Alcohol Oxidoreductases; Binding Sites; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Histidine; Molecular Weight; NAD; Yeasts | 1975 |
Microbial metabolism of aryl sulphonates a re-assessment of colorimetric methods for the determination of sulphite and their use in measuring desulphonation of aryl and alkylbenzene sulphonates.
Topics: Arylsulfonates; Colorimetry; Cysteine; Dithionitrobenzoic Acid; Rosaniline Dyes; Sulfates; Sulfites | 1975 |
Effects of disulfide bond reduction on the excitatory and inhibitory postsynaptic responses of Aplysia ganglion cells.
Topics: Acetylcholine; Acetylcholinesterase; Affinity Labels; Animals; Cholinergic Fibers; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; gamma-Aminobutyric Acid; Ganglia; Mollusca; Neural Conduction; Neural Inhibition; Nitrobenzoates; Oxidation-Reduction; Receptors, Dopamine; Receptors, Muscarinic; Receptors, Neurotransmitter; Receptors, Nicotinic; Structure-Activity Relationship | 1976 |
Generation of a free alpha-amino group by Raney nickel after 2-nitro-5-thiocyanobenzoic acid cleavage at cysteine residues: application to automated sequencing.
Topics: Amino Acid Sequence; Autoanalysis; Cysteine; Dithionitrobenzoic Acid; Globins; Glycerophosphates; Hexokinase; Nickel; Nitrobenzoates; Peptide Fragments; Phosphotransferases; Saccharomyces cerevisiae; Sulfhydryl Reagents; Thiocyanates | 1978 |
On the modification of adenosine kinase by thiols.
Topics: Adenosine Kinase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Kinetics; Mercaptoethanol; Phosphotransferases; Saccharomyces cerevisiae; Sulfhydryl Compounds | 1978 |
Reactions of pyrzdoxal 5'-phosphate, 6-aminocaproic acid, cysteine, and penicilamine. Models for reactions of Schiff base linkages in pyridoxal 5'-phosphate-requiring enymes.
Topics: Aminocaproates; Anaerobiosis; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Kinetics; Penicillamine; Pyridoxal Phosphate; Schiff Bases; Thiazoles | 1975 |
Glutamine aspartate transaminase modified at cysteine 390 with enriched carbon-13 cyanide.
Topics: Animals; Aspartate Aminotransferases; Carbon Isotopes; Chemical Phenomena; Chemistry; Cyanides; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Myocardium; Swine | 1975 |
Effect of cysteine modification on dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli B. Identification of modified residues.
Topics: Chelating Agents; Circular Dichroism; Copper; Cysteine; Dithionitrobenzoic Acid; Drug Resistance, Microbial; Escherichia coli; Mercaptoethanol; Methotrexate; Phenylmercuric Acetate; Protein Conformation; Tetrahydrofolate Dehydrogenase | 1977 |
Evidence for a single essential thiol in the yeast hexokinase molecule.
Topics: Acetylgalactosamine; Affinity Labels; Cysteine; Dithionitrobenzoic Acid; Hexokinase; Iodoacetamide; Iodoacetates; Saccharomyces cerevisiae; Thermodynamics; Thiocyanates | 1977 |
Studies of the function and location of two cysteines in the beta 2 subunit of tryptophan synthase.
Topics: Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Kinetics; Molecular Weight; Pyridoxal Phosphate; Sulfhydryl Reagents; Tryptophan Synthase | 1978 |
Conformational and ligand binding properties of the isolated domains from the beta 2 subunit of Escherichia coli tryptophan synthetase investigated by the reactivity of their cysteines.
Topics: Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Kinetics; Ligands; Macromolecular Substances; Peptide Fragments; Protein Conformation; Tryptophan Synthase | 1979 |
Inter- and intramolecular crosslinks in histone H3 induced by 5,5'-dithiobis(2-nitrobenzoic acid).
Topics: Animals; Cattle; Chemical Phenomena; Chemistry; Chickens; Cysteine; Dithionitrobenzoic Acid; Erythrocytes; Histones; Kinetics; Nitrobenzoates; Thymus Gland | 1979 |
Effects of substrates on the selective modification of the cysteinyl residues of D-amino acid transaminase.
Topics: Amino Acids; Bacillus; Cysteine; Dithionitrobenzoic Acid; Glutamates; Iodoacetates; Kinetics; Protein Binding; Transaminases | 1979 |
Primary structure of mitochondrial aspartate aminotransferase from turkey liver. Cysteine-containing peptides.
Topics: Amino Acid Sequence; Animals; Aspartate Aminotransferases; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Liver; Peptide Fragments; Protein Binding; Turkeys | 1979 |
Study of the sulfhydryl groups of beta-D-xylosidase from Bacillus pumilus.
Topics: Bacillus; Cysteine; Disulfides; Dithionitrobenzoic Acid; Glycoside Hydrolases; Kinetics; Mathematics; Xylosidases | 1978 |
Syncatalytic conformational changes in mitochondrial aspartate aminotransferases. Evidence from modification and demodification of Cys 166 in the enzyme from chicken and pig.
Topics: Amino Acids; Animals; Aspartate Aminotransferases; Chickens; Cysteine; Dithionitrobenzoic Acid; Kinetics; Mitochondria, Heart; Peptide Fragments; Swine | 1978 |
Binding of antimitotic drugs around cysteine residues of tubulin.
Topics: Binding Sites; Colchicine; Cysteine; Dithionitrobenzoic Acid; Glycoproteins; Tubulin; Vinblastine | 1978 |
Phosphoenolpyruvate carboxykinase (guanosine triphosphate) from rat liver cytosol. Separation of homogeneous forms of the enzyme with high and low activity by chromatography on agarose-hexane-guanosine triphosphate.
Topics: Adenosine Triphosphate; Animals; Chromatography, Affinity; Cysteine; Cytosol; Dithionitrobenzoic Acid; Guanosine Triphosphate; Kinetics; Ligands; Liver; Phosphoenolpyruvate Carboxykinase (GTP); Rats; Tryptophan; Tyrosine | 1978 |
A vicinal dithiol containing an essential cysteine in phosphoenolpyruvate carboxykinase (guanosine triphosphate) from cytosol of rat liver.
Topics: Animals; Arsenic; Binding Sites; Cysteine; Cystine; Cytosol; Disulfides; Dithionitrobenzoic Acid; Guanosine Triphosphate; Kinetics; Liver; Phosphoenolpyruvate Carboxykinase (GTP); Rats | 1978 |
Role of SH groups in the activity of pig phosphorylase b isoenzymes.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Isoenzymes; Muscles; Myocardium; Phosphorylase b; Phosphorylases; Rabbits; Structure-Activity Relationship; Swine | 1978 |
Molecular organization in bacterial cell membranes. Sulfhydryl groups and disulfide bridges in Streptomyces albus and Escherichia coli K 12 cytoplasmic membranes.
Topics: Bacterial Proteins; Binding Sites; Cell Membrane; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Macromolecular Substances; Protein Binding; Protein Conformation; Streptomyces; Sulfhydryl Compounds; Sulfhydryl Reagents | 1976 |
Determination of hydrogen sulfide with 5,5'-dithiobis-(2-nitrobenzoic acid), N-ethylmaleimide, and parachloromercuribenzoate.
Topics: Chloromercuribenzoates; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydrogen Sulfide; Methods; Muramidase; Nitrobenzoates; Proteins; Sulfhydryl Compounds; Sulfur | 1977 |
A comparative study of L-glutamate decarboxylase from mouse brain and bovine heart with purified preparations.
Topics: Animals; Antibody Specificity; Brain; Carboxy-Lyases; Cattle; Cysteic Acid; Cysteine; Dithionitrobenzoic Acid; Glutamate Decarboxylase; Mice; Molecular Weight; Myocardium; Pyruvates; Sulfinic Acids | 1977 |
Selective reactivity of rhodanese sulfhydryl groups with 5,5'-dithio-bis(2-nitrobenzoic acid).
Topics: Animals; Binding Sites; Catalysis; Cattle; Cyanides; Cysteine; Dithionitrobenzoic Acid; Iodoacetates; Oxidation-Reduction; Protein Conformation; Spectrophotometry; Structure-Activity Relationship; Sulfurtransferases; Thiosulfate Sulfurtransferase; Thiosulfates | 1977 |
Properties of a structurally and functionally altered form of creatine kinase produced in solutions containing chloride and nitrate.
Topics: Animals; Chlorides; Creatine Kinase; Cysteine; Dithionitrobenzoic Acid; Kinetics; Molecular Weight; Muscles; Nitrates; Protein Denaturation; Rabbits | 1977 |
A simple procedure for measuring small amounts of (14C)N-ethylmaleimide.
Topics: Binding Sites; Carbon Radioisotopes; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Kinetics; Methods; Microchemistry | 1976 |
The sulfhydryl group of monellin: its chemical reactivity and importance to the sweet taste.
Topics: Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Hydroxymercuribenzoates; Oxidation-Reduction; Plant Proteins; Protein Denaturation; Structure-Activity Relationship; Sulfhydryl Compounds; Sweetening Agents; Taste | 1976 |
Reactivity of the cysteine and tyrosine residues of aspartate transaminase from chicken heart cytosol.
Topics: Animals; Aspartate Aminotransferases; Binding Sites; Chickens; Cysteine; Cytosol; Disulfides; Dithionitrobenzoic Acid; Ethylmaleimide; Iodoacetamide; Kinetics; Mercuribenzoates; Myocardium; Protein Binding; Pyridines; Sulfhydryl Compounds; Tetranitromethane; Tyrosine | 1975 |
Chemical modification as a probe of the topography and reactivity of horse-spleen apoferritin.
Topics: Amides; Anhydrides; Animals; Apoferritins; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Ferritins; Glycine; Guanidines; Horses; Iodoacetates; Kinetics; Lysine; Maleates; Protein Binding; Protein Conformation; Spleen | 1976 |
Bovine inositol monophosphatase. Modification, identification and mutagenesis of reactive cysteine residues.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cattle; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Fluorescence Polarization; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis; Peptide Mapping; Phosphoric Monoester Hydrolases | 1992 |
A peroxidase-independent method for the quantitation of extracellular hydrogen peroxide generated by activated phagocytes in vitro.
Topics: Azides; Catalase; Cysteine; Dithionitrobenzoic Acid; Humans; Hydrogen Peroxide; Neutrophils; Peroxidase; Peroxidases; Phagocytes; Sodium Azide; Spectrophotometry; Superoxides | 1992 |
Bovine inositol monophosphatase; observation of the modification of a cysteine residue using protein fluorescence.
Topics: Animals; Brain; Cattle; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Humans; Macromolecular Substances; Molecular Weight; Mutagenesis, Site-Directed; Phosphoric Monoester Hydrolases; Rats; Spectrometry, Fluorescence | 1992 |
Role of the reactive cysteine residue in restriction endonuclease Cfr9I.
Topics: Base Sequence; Cysteine; Deoxyribonucleases, Type II Site-Specific; Dithionitrobenzoic Acid; DNA Restriction Enzymes; Ethylmaleimide; Hydrogen-Ion Concentration; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Oligonucleotides | 1992 |
Essential cysteines in 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli. Analysis by chemical modification and site-directed mutagenesis of the phenylalanine-sensitive isozyme.
Topics: 3-Deoxy-7-Phosphoheptulonate Synthase; Amino Acid Sequence; Base Sequence; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Isoenzymes; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Phenylalanine; Plasmids; Pyruvates; Sulfhydryl Reagents | 1992 |
Regulatory light-chain Cys-55 sites on the two heads of myosin can come within 2A of each other.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Mollusca; Myosins; Protein Conformation | 1992 |
[Functional role of cysteine residues of muscle succinyl-C oA-synthetase].
Topics: Animals; Chloromercuribenzoates; Columbidae; Cysteine; Dithionitrobenzoic Acid; Kinetics; Muscles; Succinate-CoA Ligases | 1992 |
Orientation factor in steady-state and time-resolved resonance energy transfer measurements.
Topics: Chemical Phenomena; Chemistry, Physical; Cysteine; Dithionitrobenzoic Acid; Energy Transfer; Fluorescence Polarization; Lysine; Mathematics; Micrococcal Nuclease; Mutation; Sulfhydryl Compounds | 1992 |
The proximity of the C-terminal domain of Escherichia coli ribosomal protein L7/L12 to L10 determined by cysteine site-directed mutagenesis and protein-protein cross-linking.
Topics: Alleles; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genetic Vectors; Kinetics; Macromolecular Substances; Mutagenesis, Site-Directed; Plasmids; Recombinant Proteins; Ribosomal Proteins; Ribosomes | 1992 |
Chemical modification of cysteinyl, lysyl and histidyl residues of mouse liver 17 beta-hydroxysteroid dehydrogenase.
Topics: 17-Hydroxysteroid Dehydrogenases; Animals; Binding Sites; Coenzymes; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Histidine; Ligands; Liver; Lysine; Mice; Protein Binding; Spectrum Analysis; Structure-Activity Relationship; Trinitrobenzenesulfonic Acid | 1992 |
Abnormal fibrinogens IJmuiden (B beta Arg14----Cys) and Nijmegen (B beta Arg44----Cys) form disulfide-linked fibrinogen-albumin complexes.
Topics: Amino Acid Sequence; Arginine; Base Sequence; Crotalid Venoms; Cysteine; Disulfides; Dithionitrobenzoic Acid; DNA; Electrophoresis, Polyacrylamide Gel; Fibrinogens, Abnormal; Gene Amplification; Humans; Immunoenzyme Techniques; Immunoglobulin G; Molecular Sequence Data; Molecular Weight; Oligodeoxyribonucleotides; Protein Binding; Serum Albumin | 1992 |
Structural properties of arrestin studied by chemical modification and circular dichroism.
Topics: Adenosine Triphosphate; Animals; Antigens; Arrestin; Calcium Chloride; Cattle; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Eye Proteins; Fluorides; Guanosine Triphosphate; Kinetics; Potassium; Potassium Chloride; Potassium Compounds; Protein Conformation | 1992 |
Interchain and intrachain crosslinking of actin thiols by a bifunctional thiol reagent.
Topics: Actins; Adenosine Diphosphate; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; Macromolecular Substances; Nitrobenzenes; Phalloidine; Protein Conformation; Sulfhydryl Compounds | 1992 |
The cysteine residues in the carboxy terminal domain of tropoelastin form an intrachain disulfide bond that stabilizes a loop structure and positively charged pocket.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithionitrobenzoic Acid; Drug Stability; Elastin; Electrophoresis, Polyacrylamide Gel; Humans; Iodoacetamide; Molecular Sequence Data; Peptide Fragments; Protein Conformation; Sequence Homology, Nucleic Acid; Tropoelastin | 1992 |
Evidence for essential histidine and cysteine residues in calcium/calmodulin-sensitive cyclic nucleotide phosphodiesterase.
Topics: Animals; Brain; Catalysis; Cattle; Cyclic Nucleotide Phosphodiesterases, Type 1; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Histidine; Hydrogen-Ion Concentration; Kinetics; Phosphoric Diester Hydrolases; Spectrophotometry, Ultraviolet | 1991 |
Partial cleavage mapping of the cytoskeletal protein vinculin. Antibody and talin binding sites.
Topics: Animals; Antibodies, Monoclonal; Binding Sites; Chickens; Cysteine; Cytoskeletal Proteins; Dithionitrobenzoic Acid; Epitopes; Gizzard, Avian; In Vitro Techniques; Molecular Weight; Peptide Mapping; Talin; Vinculin | 1991 |
On kynureninase activity.
Topics: Animals; Apoenzymes; Benzothiazoles; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Hydrolases; Kynurenine; Liver; Magnesium; Male; Phenylpyruvic Acids; Pyridoxal Phosphate; Rats; Rats, Inbred Strains; Tryptophan; Tyrosine; Xanthurenates | 1991 |
Probing the role of cysteine residues in the CheR methyltransferase.
Topics: Base Sequence; Cysteine; Dithionitrobenzoic Acid; DNA; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Kinetics; Methyltransferases; Molecular Sequence Data; Mutagenesis, Site-Directed; Sulfhydryl Compounds; Temperature | 1991 |
Thermostabilization of recombinant human and bovine CuZn superoxide dismutases by replacement of free cysteines.
Topics: Amino Acid Sequence; Animals; Base Sequence; Cattle; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Erythrocytes; Humans; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Recombinant Proteins; Saccharomyces cerevisiae; Sequence Homology, Nucleic Acid; Superoxide Dismutase; Thermodynamics | 1991 |
The identification of a structurally important cysteine residue in the glycerol dehydrogenase from Bacillus stearothermophilus.
Topics: Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Cobalt; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Geobacillus stearothermophilus; Histidine; Iodoacetates; Iodoacetic Acid; Kinetics; Macromolecular Substances; Methyl Methanesulfonate; Molecular Weight; Peptide Fragments; Sugar Alcohol Dehydrogenases; Tritium; Trypsin; Zinc | 1991 |
Determination of oxalyl thiolesters, N-oxalylcysteine and N-oxalylcysteamine in biological materials.
Topics: Animals; Bridged Bicyclo Compounds; Chromatography, High Pressure Liquid; Cysteamine; Cysteine; Dithionitrobenzoic Acid; Esters; Fluorescence; Liver; Male; Oxalates; Rats; Rats, Inbred Strains | 1991 |
Copper(II) (3,5-diisopropylsalicylate)2 oxidizes thiols to symmetrical disulfides and oxidatively converts mixtures of 5-thio-2-nitrobenzoic acid and nonsymmetrical 5-thio-2-nitrobenzoic acid disulfides to symmetrical disulfides.
Topics: Cysteine; Disulfides; Dithionitrobenzoic Acid; Drug Interactions; Glutathione; Nitrobenzoates; Oxidation-Reduction; Penicillamine; Salicylates; Sulfhydryl Compounds | 1991 |
Effect of cysteine replacements at positions 13 and 50 on metallothionein structure.
Topics: Animals; Cadmium; Circular Dichroism; Cricetinae; Cricetulus; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Kinetics; Metallothionein; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Recombinant Proteins; Saccharomyces cerevisiae; Spectrophotometry, Ultraviolet | 1991 |
A modified technique for the measurement of sulfhydryl groups oxidized by reactive oxygen intermediates.
Topics: Catalase; Cysteine; Dithionitrobenzoic Acid; Free Radicals; Hydrogen Peroxide; Hypoxanthine; Hypoxanthines; Metallothionein; Nitrophenols; Oxygen; Sulfhydryl Compounds; Xanthine Oxidase | 1990 |
Site-directed mutagenesis of glycine-14 and two "critical" cysteinyl residues in Drosophila alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Animals; Cysteine; Dithionitrobenzoic Acid; DNA; Drosophila melanogaster; Enzyme Activation; Gene Expression; Glycine; Kinetics; Mutation; Polymerase Chain Reaction; Protein Denaturation | 1990 |
Xenopus transcription factor IIIA. Evidence for heterogeneity of Zn2+ binding affinities and specific labeling of cysteine 287.
Topics: Animals; Binding Sites; Chromatography, Ion Exchange; Cysteine; Dithionitrobenzoic Acid; DNA-Binding Proteins; Female; Fluorescent Dyes; Kinetics; Metalloproteins; Molecular Weight; Naphthalenesulfonates; Ovary; Resorcinols; Transcription Factor TFIIIA; Transcription Factors; Xenopus laevis; Zinc | 1990 |
Chemical state of the cysteine residues in the Neurospora crassa plasma membrane H(+)-ATPase.
Topics: Cell Membrane; Chromatography, Gel; Chromatography, High Pressure Liquid; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Iodoacetates; Iodoacetic Acid; Kinetics; Neurospora; Neurospora crassa; Peptide Fragments; Proton-Translocating ATPases; Sulfhydryl Compounds; Trypsin | 1990 |
Multiple modes of activation of latent human fibroblast collagenase: evidence for the role of a Cys73 active-site zinc complex in latency and a "cysteine switch" mechanism for activation.
Topics: Amino Acid Sequence; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Fibroblasts; Gingiva; Humans; Kinetics; Microbial Collagenase; Models, Structural; Molecular Sequence Data; Protein Binding; Protein Conformation; Zinc | 1990 |
Identification of different classes of nonessential sulfhydryl groups in Escherichia coli adenylosuccinate synthetase.
Topics: Adenylosuccinate Synthase; Binding Sites; Chromatography, High Pressure Liquid; Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Electron Spin Resonance Spectroscopy; Escherichia coli; Ethylmaleimide; Kinetics; Ligases; Nitrobenzoates; Peptide Fragments; Sulfhydryl Compounds; Trypsin | 1990 |
Sulfhydryl chemistry of Salmonella typhimurium phosphoribosylpyrophosphate synthetase: identification of two classes of cysteinyl residues.
Topics: Amino Acid Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Iodoacetamide; Kinetics; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Phosphotransferases; Ribose-Phosphate Pyrophosphokinase; Salmonella typhimurium; Sulfhydryl Reagents | 1990 |
Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification.
Topics: Amino Acid Sequence; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Endoribonucleases; Escherichia coli; Ethylmaleimide; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Mapping; Ribonuclease H; Serine Endopeptidases; Structure-Activity Relationship | 1990 |
2,4-Dinitrophenyl [14C]cysteinyl disulfide allows selective radioactive labeling of protein thiols under spectrophotometric control.
Topics: Actins; Animals; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Hydrolysis; Indicators and Reagents; Muscles; Peptides; Proteins; Rabbits; Sulfhydryl Compounds | 1990 |
Chemical modification of xylanases: evidence for essential tryptophan and cysteine residues at the active site.
Topics: Bacillus; Binding Sites; Bromosuccinimide; Cysteine; Dithionitrobenzoic Acid; Enzyme Inhibitors; Glycoside Hydrolases; Hot Temperature; Hydroxymercuribenzoates; Kinetics; Streptomyces; Tryptophan; Xylan Endo-1,3-beta-Xylosidase | 1990 |
The carboxyl-terminal region of thioesterase II participates in the interaction with fatty acid synthase. Use of electrospray ionization mass spectrometry to identify a carboxyl-terminally truncated form of the enzyme.
Topics: Acyl Coenzyme A; Amino Acid Sequence; Animals; Borates; Catalysis; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Fatty Acid Synthases; Female; Mammary Glands, Animal; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Peptide Fragments; Potassium Cyanide; Rats; Thiolester Hydrolases | 1990 |
Site-directed mutagenesis of the cysteinyl residues and the active-site serine residue of bacterial D-amino acid transaminase.
Topics: Bacillus; Base Sequence; Binding Sites; Cysteine; D-Alanine Transaminase; Dithionitrobenzoic Acid; Glycine; Kinetics; Macromolecular Substances; Molecular Sequence Data; Mutation; Serine; Transaminases | 1989 |
Functional cysteinyl residues in human placental aldose reductase.
Topics: Aldehyde Reductase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; Female; Humans; Kinetics; Models, Theoretical; Placenta; Pregnancy; Protein Binding; Substrate Specificity; Sugar Alcohol Dehydrogenases | 1989 |
Conformational modification of muscle phosphofructokinase from Jaculus orientalis upon ligand binding.
Topics: Adenosine Monophosphate; Adenosine Triphosphate; Allosteric Regulation; Animals; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Fructosephosphates; Inosine Triphosphate; Muscles; Phosphofructokinase-1; Protein Conformation; Rodentia; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 1989 |
Conformational change in beef-heart mitochondrial F1 ATPase to ATP synthesis mode induced by dimethylsulfoxide and ATP revealed by sulfhydryl group labeling.
Topics: Adenosine Triphosphate; Animals; Cattle; Cysteine; Dimethyl Sulfoxide; Dithionitrobenzoic Acid; Guanidine; Guanidines; Mitochondria, Heart; Protein Conformation; Proton-Translocating ATPases | 1989 |
Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities.
Topics: Alkylation; Bacterial Proteins; Base Sequence; Chromatography, High Pressure Liquid; Cysteine; Deoxyribonuclease I; Dithionitrobenzoic Acid; DNA-Binding Proteins; DNA, Bacterial; Gene Expression Regulation; Iodoacetamide; Kinetics; Ligands; Mercury; Metalloproteins; Methylation; Mutation; Operator Regions, Genetic; Promoter Regions, Genetic; Protein Binding; Thermodynamics; Transcription, Genetic | 1989 |
Reaction of lecithin cholesterol acyltransferase with water-soluble substrates.
Topics: Apolipoprotein C-II; Apolipoproteins C; Butyrates; Cysteine; Dithionitrobenzoic Acid; Humans; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Phosphatidylcholine-Sterol O-Acyltransferase; Sodium Chloride; Solubility; Substrate Specificity | 1989 |
Studies on fatty-acid-binding proteins. The purification of rat liver fatty-acid-binding protein and the role of cysteine-69 in fatty acid binding.
Topics: Animals; Carrier Proteins; Chromatography, Affinity; Cysteine; Dithionitrobenzoic Acid; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids; Heme; Ligands; Liver; Neoplasm Proteins; Nerve Tissue Proteins; Rats; Rats, Inbred Strains | 1989 |
Reactivities of sulfhydryl groups in native and metal-free aminoacylase I.
Topics: Amidohydrolases; Apoenzymes; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Kinetics; Metalloproteins; Protein Binding; Zinc | 1989 |
Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coli.
Topics: Allosteric Regulation; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Enzyme Reactivators; Escherichia coli; Glucose-6-Phosphate Isomerase; Hydrocarbons, Iodinated; Kinetics; Periodic Acid; Protein Denaturation; Structure-Activity Relationship; Sulfhydryl Compounds; Urea | 1987 |
Cysteine residues at the active site of glutamine synthetase from spinach leaves.
Topics: Binding Sites; Catalysis; Chemical Phenomena; Chemistry; Chloroplasts; Cysteine; Dithionitrobenzoic Acid; Glutamate-Ammonia Ligase; Plants; Sulfhydryl Compounds | 1985 |
Evidence for a reactive cysteine at the nucleotide binding site of spinach ribulose-5-phosphate kinase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Ethanolamines; Ethylmaleimide; Kinetics; Nucleotides; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Plants | 1985 |
A comparison of the cyclic nucleotide-dependent protein kinases using chemical cleavage at tryptophan and cysteine.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, Gel; Cyclic AMP; Cyclic GMP; Cysteine; Disulfides; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Kinetics; Molecular Weight; Protein Kinases; Tryptophan | 1985 |
Chemical modification of NADPH-cytochrome P-450 reductase. Presence of a lysine residue in the rat hepatic enzyme as the recognition site of 2'-phosphate moiety of the cofactor.
Topics: Animals; Binding Sites; Coenzymes; Cysteine; Cytochrome c Group; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Liver; Lysine; NADPH-Ferrihemoprotein Reductase; Oxidation-Reduction; Phosphates; Rats; Trinitrobenzenesulfonic Acid | 1986 |
Effects of sulfhydryl agents on neuromuscular transmission in the presence or absence of cadmium.
Topics: Animals; Cadmium; Calcium; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; In Vitro Techniques; Mice; Neuromuscular Junction; Sulfhydryl Compounds; Synaptic Transmission | 1986 |
Role of cysteine residues in glutathione synthetase from Escherichia coli B. Chemical modification and oligonucleotide site-directed mutagenesis.
Topics: Alanine; Amino Acids; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Codon; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Ethylmaleimide; Genes, Bacterial; Glutathione Synthase; Mutation; p-Chloromercuribenzoic Acid; Peptide Synthases; Plasmids; Sulfhydryl Compounds; Thiocyanates; Transformation, Bacterial | 1988 |
Chemical modification of lysine residues at active-site of human placental estradiol 17 beta-dehydrogenase.
Topics: 17-Hydroxysteroid Dehydrogenases; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Estradiol Dehydrogenases; Female; Humans; Lysine; Placenta; Pregnancy; Structure-Activity Relationship; Sulfhydryl Reagents; Trinitrobenzenesulfonic Acid | 1988 |
The binding of factor Va to phospholipid vesicles.
Topics: Animals; Calcium; Cattle; Cysteine; Dithionitrobenzoic Acid; Factor V; Factor Va; Factor Xa; Fluorescence Polarization; Fluorescent Dyes; Kinetics; Liposomes; Maleimides; Phosphatidylcholines; Phosphatidylserines; Serine Endopeptidases; Spectrometry, Fluorescence; Sulfhydryl Compounds; Sulfhydryl Reagents | 1988 |
Recombinant rat liver guanidinoacetate methyltransferase: reactivity and function of sulfhydryl groups.
Topics: Animals; Binding Sites; Cysteine; Disulfides; Dithionitrobenzoic Acid; Guanidinoacetate N-Methyltransferase; In Vitro Techniques; Iodoacetates; Iodoacetic Acid; Kinetics; Liver; Methyltransferases; Rats | 1988 |
Conformational features of bovine heart mitochondrial transhydrogenase.
Topics: Animals; Binding Sites; Cattle; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; In Vitro Techniques; Mitochondria, Heart; NADH, NADPH Oxidoreductases; NADP; NADP Transhydrogenases; Protein Conformation; Sulfhydryl Reagents | 1988 |
Identification of the reactive cysteines of Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase and their nonessentiality for enzymatic catalysis.
Topics: 3-Phosphoshikimate 1-Carboxyvinyltransferase; Alkyl and Aryl Transferases; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Glycine; Glyphosate; Kinetics; Shikimic Acid; Transferases | 1988 |
Importance of sulfhydryl group for rabbit gastric lipase activity.
Topics: Animals; Cysteine; Disulfides; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Kinetics; Lipase; Pyridines; Rabbits; Stomach; Sulfhydryl Reagents; Triglycerides | 1988 |
Modification of erythrocyte enzyme activities by persulfides and methanethiol: possible regulatory role.
Topics: Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Erythrocytes; Humans; Kinetics; Pyridoxal; Pyruvate Kinase; Sulfhydryl Compounds; Sulfides | 1987 |
Formation of mixed disulfide adducts at cysteine-281 of the lactose repressor protein affects operator and inducer binding parameters.
Topics: Cysteine; Cystine; Dansyl Compounds; Disulfides; Dithionitrobenzoic Acid; Escherichia coli; Isopropyl Thiogalactoside; Kinetics; Methyl Methanesulfonate; Nitrobenzoates; Operator Regions, Genetic; Protein Binding; Repressor Proteins; Transcription Factors | 1986 |
Active site of the enzyme which demethylates receptors during bacterial chemotaxis.
Topics: Bacterial Physiological Phenomena; Bacterial Proteins; Base Sequence; Binding Sites; Carboxylic Ester Hydrolases; Chemoreceptor Cells; Chemotaxis; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Mercaptoethanol; Mutation; Salmonella typhimurium | 1987 |
Differences in local conformation around cysteine residues in alpha alpha, alpha beta, and beta beta rabbit skeletal tropomyosin.
Topics: Animals; Cross-Linking Reagents; Cysteine; Disulfides; Dithionitrobenzoic Acid; Hydrolysis; Peptide Fragments; Protein Conformation; Rabbits; Tropomyosin; Trypsin; Urea | 1987 |
The determination of glutathione, cyst(e)ine, and other thiols and disulfides in biological samples using high-performance liquid chromatography with dual electrochemical detection.
Topics: Aedes; Animals; Chromatography, High Pressure Liquid; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Electrochemistry; Female; Glutathione; Hippocampus; Humans; Liver; Plants; Rats; Sulfhydryl Compounds | 1987 |
Cyst(e)ine residues of bovine white-matter proteolipid proteins. Role of disulphides in proteolipid conformation.
Topics: Animals; Cattle; Circular Dichroism; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Formates; Iodoacetates; Nerve Tissue; Protein Conformation; Proteolipids; Spectrometry, Fluorescence | 1987 |
Function and reactivity of sulfhydryl groups of rat liver glycine methyltransferase.
Topics: Amino Acids; Animals; Carbon Radioisotopes; Cysteine; Dithionitrobenzoic Acid; Glycine N-Methyltransferase; Iodoacetates; Iodoacetic Acid; Kinetics; Liver; Methyltransferases; Nitrobenzoates; Peptide Fragments; Rats; Trypsin | 1987 |
Characterization of two cysteine residues in cytochrome P-450scc: chemical identification of the heme-binding cysteine residue.
Topics: Adrenal Cortex; Animals; Binding Sites; Cattle; Cysteine; Cytochrome P-450 Enzyme System; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Heme; Isoenzymes; Kinetics; Mathematics; Molecular Weight; Structure-Activity Relationship | 1986 |
Human plasma lecithin-cholesterol acyltransferase. An elucidation of the catalytic mechanism.
Topics: Acyltransferases; Cysteine; Dithionitrobenzoic Acid; Humans; Kinetics; Liposomes; Phosphatidylcholine-Sterol O-Acyltransferase; Phospholipases A; Phospholipases A2; Protein Binding; Proteolipids | 1986 |
Argininosuccinate synthetase: essential role of cysteine and arginine residues in relation to structure and mechanism of ATP activation.
Topics: Adenosine Triphosphate; Animals; Arginine; Argininosuccinate Synthase; Binding Sites; Cattle; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Kinetics; Ligases; Liver; Phenylglyoxal; Protein Binding | 1985 |
Characterization of fumarate reductase from baker's yeast: essential sulfhydryl group for binding of FAD.
Topics: Apoenzymes; Cysteine; Dithionitrobenzoic Acid; Flavin-Adenine Dinucleotide; Kinetics; Saccharomyces cerevisiae; Succinate Dehydrogenase | 1985 |
The phosphate group of 3-phosphoglycerate accounts for conformational changes occurring on binding to 3-phosphoglycerate kinase. Enzyme inhibition and thiol reactivity studies.
Topics: Animals; Binding Sites; Binding, Competitive; Cysteine; Dithionitrobenzoic Acid; Glyceric Acids; Kinetics; Molecular Conformation; Phosphates; Phosphoglycerate Kinase; Protein Conformation; Structure-Activity Relationship | 1986 |
Modification of the calmodulin-stimulated phosphatase, calcineurin, by sulfhydryl reagents.
Topics: Animals; Binding Sites; Calcium; Calmodulin-Binding Proteins; Cattle; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Macromolecular Substances; Manganese; Nickel; Sulfhydryl Reagents | 1986 |
Studies on the identity of the heme-binding cysteinyl residue in rabbit liver microsomal cytochrome P-450 isozyme 2.
Topics: Animals; Binding Sites; Bridged Bicyclo Compounds; Cysteine; Cytochrome P-450 Enzyme System; Dithionitrobenzoic Acid; Heme; Isoenzymes; Kinetics; Microsomes, Liver; Rabbits | 1985 |
Modification of the catalytic and regulatory properties of beef heart AMP-deaminase by DTNB treatment.
Topics: Allosteric Regulation; AMP Deaminase; Animals; Cattle; Cysteine; Dithionitrobenzoic Acid; Myocardium; Nucleotide Deaminases; Oxidation-Reduction; Protein Conformation | 1985 |
Labeling of cysteine-containing peptides with 2-nitro-5-thiobenzoic acid.
Topics: Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Nitrobenzoates; Peptides; Sulfhydryl Compounds | 1985 |
Removal of an N-terminal peptide from mitochondrial aspartate aminotransferase abolishes its interactions with mitochondria in vitro.
Topics: Amino Acids; Animals; Aspartate Aminotransferases; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Mitochondria, Liver; Peptide Fragments; Protein Conformation; Rats; Trypsin | 1985 |
Effect of the lactoperoxidase system on lipoprotein lipase activity and lipolysis in milk.
Topics: Animals; Cattle; Cysteine; Dithionitrobenzoic Acid; Fatty Acids, Nonesterified; Female; Lactoperoxidase; Lipolysis; Lipoprotein Lipase; Milk; Peroxidases; Tetrathionic Acid; Thiocyanates | 1985 |
Inhibition of the functional interaction between fatty acid synthetase and thioesterase II by modification of a single cysteine thiol on the thioesterase.
Topics: Animals; Binding Sites; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Fatty Acid Synthases; Female; Hydrolysis; Kinetics; Methyl Methanesulfonate; Protein Binding; Rats; Sulfhydryl Compounds; Thiolester Hydrolases | 1985 |
Periodate inactivates muscle glycogen phosphorylase by modifying the enzyme active site.
Topics: Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Kinetics; Muscles; Periodic Acid; Phosphorylase b; Phosphorylases; Protein Binding; Rabbits | 1983 |
Two sulfhydryl groups near the active site of thiolase I from porcine heart: modification of thiolase with the fluorescent thiol reagent S-mercurio-N-dansyl-L-cysteine.
Topics: Acetyl-CoA C-Acetyltransferase; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Iodoacetamide; Kinetics; Myocardium; Spectrometry, Fluorescence; Sulfhydryl Reagents; Swine | 1982 |
Complexes of serum albumin and cis-dichlorodiammineplatinum (II). The role of cysteine 34 as a nucleophilic entering group and evidence for reaction between bound platinum and a second macromolecule.
Topics: alpha-Macroglobulins; Animals; Binding, Competitive; Cattle; Chemical Phenomena; Chemistry; Cisplatin; Cysteine; Dithionitrobenzoic Acid; Platinum; Serum Albumin | 1983 |
Interaction with protein SH groups could be involved in adriamycin cardiotoxicity.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Doxorubicin; Enzyme Inhibitors; Heart; Hexokinase; Isocitrate Dehydrogenase; Malate Dehydrogenase; Male; Myocardium; Phosphofructokinase-1; Phosphogluconate Dehydrogenase; Protein Binding; Rats; Rats, Inbred Strains; Sulfhydryl Compounds | 1984 |
Pig liver phosphomevalonate kinase. 2. Participation of cysteinyl and lysyl groups in catalysis.
Topics: Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Kinetics; Liver; Lysine; Mevalonic Acid; Phosphotransferases; Phosphotransferases (Phosphate Group Acceptor); Pyridoxal Phosphate; Swine | 1980 |
The reactivity of thiol groups in bovine heart cytochrome c oxidase towards 5,5'-dithiobis(2-nitrobenzoic acid).
Topics: Amino Acid Sequence; Amino Acids; Animals; Binding Sites; Cattle; Cysteine; Dithionitrobenzoic Acid; Electron Transport Complex IV; Kinetics; Myocardium; Nitrobenzoates; Protein Binding; Sulfhydryl Compounds | 1982 |
Ca2+-induced conformational changes of 20,000 dalton light chain of vertebrate striated muscle myosins.
Topics: Animals; Calcium; Chemical Phenomena; Chemistry; Chickens; Cysteine; Dithionitrobenzoic Acid; Electron Spin Resonance Spectroscopy; In Vitro Techniques; Muscles; Myosins; Oxidation-Reduction; Phosphorylation; Protein Conformation; Rabbits; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 1983 |
Cysteinyl residues of Escherichia coli recA protein.
Topics: Amino Acids; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Kinetics; Maleimides; Rec A Recombinases; Spectrophotometry; Sulfhydryl Reagents | 1984 |
Chorismate mutase/prephenate dehydrogenase from Escherichia coli K12: purification, characterization, and identification of a reactive cysteine.
Topics: Amino Acid Sequence; Centrifugation; Chemical Phenomena; Chemistry; Chorismate Mutase; Chromatography, Affinity; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Iodoacetamide; Isomerases; Kinetics; Molecular Weight; Oxidoreductases; Peptide Fragments; Prephenate Dehydrogenase; Thermolysin; Trypsin | 1984 |
Partial amino-acid sequence and cysteine reactivities of cytosolic aspartate aminotransferase from horse heart.
Topics: Amino Acid Sequence; Animals; Aspartate Aminotransferases; Chymotrypsin; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Horses; Isoenzymes; Myocardium; Peptide Fragments; Swine | 1984 |
[Role of the sulfhydryl groups of phosphorylase kinase].
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Kinetics; Muscles; Nitrobenzoates; Phosphorylase Kinase; Rabbits; Sulfhydryl Compounds | 1983 |
Nonspecific inhibition of enzymes by p-bromophenacyl bromide. Inhibition of human platelet phospholipase C and modification of sulfhydryl groups.
Topics: Acetophenones; Blood Platelets; Cysteine; Cytosol; Dithionitrobenzoic Acid; Humans; Kinetics; Methyl Methanesulfonate; Phospholipases; Sulfhydryl Compounds; Type C Phospholipases | 1984 |
The reactivity of sulfhydryl groups of yeast DNA dependent RNA polymerase I.
Topics: Chloromercuribenzoates; Cysteine; Dithionitrobenzoic Acid; DNA-Directed RNA Polymerases; Ethylmaleimide; Kinetics; Mercaptoethanol; p-Chloromercuribenzoic Acid; Protein Binding; RNA Polymerase I; Saccharomyces cerevisiae | 1982 |
Evidence for the importance of cysteine and arginine residues in Pseudomonas fluorescens UK-1 pantoate dehydrogenase.
Topics: Alcohol Oxidoreductases; Arginine; Chloromercuribenzoates; Cysteine; Dithionitrobenzoic Acid; Hydroxybutyrates; Iodoacetates; NAD; Phenylglyoxal; Pseudomonas fluorescens | 1980 |
Electrostatic influence of local cysteine environments on disulfide exchange kinetics.
Topics: Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Kinetics; Mathematics; Nitrobenzoates; Osmolar Concentration; Pepsin A; Peptide Fragments; Protein Binding; Proteins | 1981 |
Specific cleavage of glucosephosphate isomerases at cysteinyl residues using 2-nitro-5-thiocyanobenzoic acid: analyses of peptides eluted from polyacrylamide gels and localization of active site histidyl and lysyl residues.
Topics: Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Female; Glucose-6-Phosphate Isomerase; Histidine; Humans; Lysine; Muscles; Peptide Fragments; Placenta; Pregnancy; Sulfhydryl Reagents; Swine; Thiocyanates | 1981 |
A modification of the 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) method for the determination of the sulphhydryl content of human serum albumin.
Topics: Adult; Cysteine; Dithionitrobenzoic Acid; Humans; Kinetics; Methods; Middle Aged; Nitrobenzoates; Serum Albumin; Sulfhydryl Compounds; Temperature | 1983 |
Differential reactivity of the functional sulfhydryl groups of cysteine-32 and cysteine-35 present in the reduced form of thioredoxin from Escherichia coli.
Topics: Amino Acids; Bacterial Proteins; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Hydrogen-Ion Concentration; Iodoacetates; Kinetics; Protein Binding; Thioredoxin-Disulfide Reductase; Thioredoxins | 1980 |
Selective arylation of cysteine-237 of rabbit muscle aldolase with 4-chloro-7-nitrobenzofurazan.
Topics: 4-Chloro-7-nitrobenzofurazan; Animals; Cysteine; Dithionitrobenzoic Acid; Fluorescent Dyes; Fructose-Bisphosphate Aldolase; Kinetics; Macromolecular Substances; Muscles; Oxadiazoles; Rabbits; Spectrophotometry | 1982 |
Human liver acid phosphatases: cysteine residues of the low-molecular-weight enzyme.
Topics: Acid Phosphatase; Cysteine; Dithionitrobenzoic Acid; Fluoresceins; Humans; Kinetics; Liver; Molecular Weight; Organomercury Compounds; Spectrometry, Fluorescence; Sulfhydryl Reagents | 1982 |
Physicochemical properties and states of sulfhydryl groups of uricase from Candida utilis.
Topics: Amino Acids; Candida; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Iodobenzoates; Isoelectric Point; Molecular Weight; Protein Conformation; Protein Denaturation; Sulfhydryl Compounds; Urate Oxidase | 1982 |
Cysteine in the manganous-isocitrate binding site of pig heart TPN-specific isocitrate dehydrogenase. II. Identification of a critical cysteine-containing tryptic peptide derived from the thiocyano enzyme.
Topics: Amino Acids; Animals; Binding Sites; Cyanides; Cysteine; Dithionitrobenzoic Acid; Isocitrate Dehydrogenase; Isocitrates; Manganese; Myocardium; NADP; Peptide Fragments; Potassium Cyanide; Swine; Trypsin | 1981 |
Cysteine in the manganous-isocitrate binding site of pig heart TPN-specific isocitrate dehydrogenase. I. Kinetics of chemical modification and properties of thiocyano enzyme.
Topics: Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Isocitrate Dehydrogenase; Isocitrates; Kinetics; Manganese; Myocardium; NADP; Nitrobenzoates; Potassium Cyanide; Swine | 1981 |
A large-scale preparation and some physicochemical properties of recA protein.
Topics: Bacterial Proteins; Chromatography, Gel; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Macromolecular Substances; Protein Denaturation; Rec A Recombinases; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet | 1981 |
Subunit stoichiometry of tobacco ribulose 1.5-bisphosphate carboxylase.
Topics: Amino Acids; Carboxy-Lyases; Cysteine; Dithionitrobenzoic Acid; Macromolecular Substances; Molecular Weight; Nicotiana; Plants; Plants, Toxic; Ribulose-Bisphosphate Carboxylase | 1980 |
Reactivity of sulfhydryl groups of the flavoenzyme D-lactate dehydrogenase and effect on catalytic activity.
Topics: Chloromercuribenzoates; Cysteine; Disulfides; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Kinetics; L-Lactate Dehydrogenase; Molecular Weight; Nitrobenzoates; Protein Binding; Veillonellaceae | 1980 |
Zinc and cysteine residues in the active site of bovine liver delta-aminolevulinic acid dehydratase.
Topics: Animals; Binding Sites; Cattle; Cysteine; Dithionitrobenzoic Acid; Kinetics; Lead; Liver; Porphobilinogen Synthase; Protein Binding; Zinc | 1980 |
Large scale purification and structural characterization of squalene and sterol carrier protein.
Topics: Amino Acids; Animals; Carrier Proteins; Cysteine; Dithionitrobenzoic Acid; Fatty Acids; Female; Guanidines; Iodoacetates; Liver; Molecular Weight; Peptide Fragments; Rats; Spectrophotometry, Ultraviolet; Sterols; Trypsin | 1981 |
Nature and environment of the sulfhydryls of membrane-associated D-lactate dehydrogenase of Escherichia coli.
Topics: Bacterial Outer Membrane Proteins; Cysteine; Disulfides; Dithionitrobenzoic Acid; Escherichia coli; Kinetics; L-Lactate Dehydrogenase; Mutagenesis, Site-Directed; Nitrobenzoates; Serine; Sulfhydryl Compounds; Sulfhydryl Reagents | 1995 |
Quantification of cysteinyl sulfhydryl residues in peptides and proteins by ESI-MS or MALDI-MS.
Topics: Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Guanidine; Guanidines; Indicators and Reagents; Mass Spectrometry; Peptides; Plant Proteins; Plants; Pyridines; Sulfhydryl Compounds | 1995 |
Assay for thiols based on reactivation of papain.
Topics: Animals; Binding Sites; Cysteine; Disulfides; Dithionitrobenzoic Acid; Edetic Acid; Fabaceae; Fructose-Bisphosphate Aldolase; Indicators and Reagents; Kinetics; Methyl Methanesulfonate; Muscle, Skeletal; Oxidation-Reduction; Papain; Plants, Medicinal; Proteins; Rabbits; Sensitivity and Specificity; Serum Albumin, Bovine; Spectrophotometry; Sulfhydryl Compounds; Urease | 1995 |
Mechanistic studies on CDP-6-deoxy-delta 3,4-glucoseen reductase: the role of cysteine residues in catalysis as probed by chemical modification and site-directed mutagenesis.
Topics: Amino Acid Sequence; Base Sequence; Binding Sites; Catalysis; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Escherichia coli; Ethylmaleimide; Gene Expression; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Oxidoreductases; Recombinant Proteins; Structure-Activity Relationship; Trypsin; Yersinia pseudotuberculosis | 1995 |
A spectrophotometric method for quantitative determination of allicin and total garlic thiosulfinates.
Topics: Anti-Infective Agents; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithionitrobenzoic Acid; Garlic; Indicators and Reagents; Plants, Medicinal; Spectrophotometry; Sulfinic Acids; Sulfonic Acids | 1995 |
Identification of the reactive cysteine in clostridial glutamate dehydrogenase by site-directed mutagenesis and proof that this residue is not strictly essential.
Topics: Base Sequence; Cloning, Molecular; Clostridium; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Glutamate Dehydrogenase; Molecular Sequence Data; Mutagenesis, Site-Directed; Serine; Structure-Activity Relationship | 1994 |
Secretion of cysteine and glutathione from mucosa to lumen in rat small intestine.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Eating; gamma-Glutamyltransferase; Glutathione; In Vitro Techniques; Intestinal Mucosa; Intestine, Small; Male; Rats; Rats, Sprague-Dawley; Sulfhydryl Compounds; Tissue Distribution | 1994 |
Modulation of the activity of mitochondrial aspartate aminotransferase H352C by the redox state of the engineered interdomain disulfide bond.
Topics: Animals; Aspartate Aminotransferases; Base Sequence; Catalysis; Chickens; Cysteine; Disulfides; Dithionitrobenzoic Acid; Kinetics; Mitochondria; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Conformation; Protein Engineering; Recombinant Proteins; Structure-Activity Relationship | 1994 |
Investigation of the nature of the two metal-binding sites in 5-aminolaevulinic acid dehydratase from Escherichia coli.
Topics: Amino Acid Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cobalt; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Ethylmaleimide; Hydrogen-Ion Concentration; Magnesium; Metals; Molecular Sequence Data; Peptide Mapping; Porphobilinogen Synthase; Spectrometry, Fluorescence; Spectrophotometry, Atomic; Zinc | 1994 |
Inactivation of a cytosolic phospholipase A2 by thiol-modifying reagents: cysteine residues as potential targets of phospholipase A2.
Topics: Amino Acid Sequence; Cell Line; Cysteine; Cytosol; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Stability; Humans; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Molecular Sequence Data; Phospholipases A; Phospholipases A2; Recombinant Proteins; Sulfhydryl Reagents | 1994 |
Studies on acid soluble thiols in the human gastric juice.
Topics: Chromatography, Gel; Chromatography, High Pressure Liquid; Colorimetry; Cysteine; Dialysis; Dithionitrobenzoic Acid; Gastric Juice; Glutathione; Humans; Hydrogen-Ion Concentration; Solubility | 1994 |
Reactivity of cysteine-67 of the human immunodeficiency virus-1 protease: studies on a peptide spanning residues 59 to 75.
Topics: Amino Acid Sequence; Cysteine; Dithionitrobenzoic Acid; HIV Protease; HIV-1; Hydrogen-Ion Concentration; Molecular Sequence Data; Oxidation-Reduction; Peptides | 1994 |
Mutagenesis of the cyclic AMP receptor protein of Escherichia coli: targeting positions 83, 127 and 128 of the cyclic nucleotide binding pocket.
Topics: Base Sequence; beta-Galactosidase; Binding Sites; Cross-Linking Reagents; Cyclic AMP; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Glycine; Isoleucine; Molecular Sequence Data; Mutagenesis; Receptors, Cyclic AMP; Serine; Structure-Activity Relationship; Threonine; Transcription, Genetic | 1994 |
A disulfide crosslink between Cys98 of troponin-C and Cys133 of troponin-I abolishes the activity of rabbit skeletal troponin.
Topics: Actomyosin; Amino Acid Sequence; Animals; Ca(2+) Mg(2+)-ATPase; Calcium-Transporting ATPases; Cross-Linking Reagents; Cysteine; Disulfides; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Kinetics; Muscles; Rabbits; Time Factors; Troponin; Troponin I | 1994 |
Differential inactivation of rabbit and yeast triosephosphate isomerase: effect of oxidations produced by chloramine-T.
Topics: Animals; Chloramines; Circular Dichroism; Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Indicators and Reagents; Kinetics; Methionine; Molecular Weight; Muscles; Oxidation-Reduction; Peptide Fragments; Peptide Mapping; Protein Conformation; Rabbits; Saccharomyces cerevisiae; Spectrophotometry, Ultraviolet; Tosyl Compounds; Triose-Phosphate Isomerase | 1994 |
Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Erythrocytes; Ethylmaleimide; Humans; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; Peptide Fragments; Phosphoric Monoester Hydrolases; Protein Binding; Sulfhydryl Reagents | 1994 |
Cysteine 254 can cooperate with active site cysteine 247 in reactivation of 5,5'-dithiobis(2-nitrobenzoic acid)-inactivated rhodanese as determined by site-directed mutagenesis.
Topics: Animals; Binding Sites; Cattle; Cysteine; Disulfides; Dithionitrobenzoic Acid; Enzyme Activation; Enzyme Reactivators; Mercaptoethanol; Mutagenesis, Site-Directed; Pyridines; Sulfhydryl Reagents; Sulfides; Thiosulfate Sulfurtransferase | 1994 |
Intestinal fatty acid binding protein: characterization of mutant proteins containing inserted cysteine residues.
Topics: Animals; Carrier Proteins; Cysteine; Disulfides; Dithionitrobenzoic Acid; Drug Stability; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Mutagenesis, Site-Directed; Neoplasm Proteins; Nerve Tissue Proteins; Protein Denaturation; Protein Structure, Secondary; Pyridines; Rats; Spectrometry, Fluorescence; Sulfhydryl Compounds | 1993 |
Cysteine mutants of light chain-2 form disulfide bonds in skeletal muscle myosin.
Topics: Animals; Base Sequence; Cations, Divalent; Chickens; Cysteine; Disulfides; Dithionitrobenzoic Acid; In Vitro Techniques; Molecular Sequence Data; Mutagenesis, Site-Directed; Myosins; Oligodeoxyribonucleotides; Oxidation-Reduction; Structure-Activity Relationship | 1993 |
Restoring free sulfhydryl groups in synthetic peptides.
Topics: Amino Acid Sequence; Borohydrides; Cysteine; Disulfides; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Indicators and Reagents; Molecular Sequence Data; Oligopeptides; Sulfhydryl Compounds | 1993 |
Effect on ligand binding of arginine mutations in recombinant rat liver fatty acid-binding protein.
Topics: Acyl Coenzyme A; Animals; Arginine; Carrier Proteins; Cysteine; Dansyl Compounds; Dithionitrobenzoic Acid; Drug Stability; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids; Fluorescent Dyes; Liver; Mutation; Neoplasm Proteins; Nerve Tissue Proteins; Oleic Acid; Oleic Acids; Rats; Recombinant Proteins; Spectrometry, Fluorescence; Structure-Activity Relationship | 1994 |
Zinc-free and reduced T4 gene 32 protein binds single-stranded DNA weakly and fails to stimulate UvsX-catalyzed homologous pairing.
Topics: Apoproteins; Binding Sites; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; DNA-Binding Proteins; DNA, Single-Stranded; Edetic Acid; Methylation; Oxidation-Reduction; Protein Binding; Protein Conformation; Recombinant Proteins; Recombination, Genetic; Structure-Activity Relationship; Viral Proteins; Zinc | 1994 |
Reactivity of cysteine residues in the protease from human immunodeficiency virus: identification of a surface-exposed region which affects enzyme function.
Topics: Copper; Cysteine; Dithionitrobenzoic Acid; HIV Protease; HIV Protease Inhibitors; Peptide Mapping; Protein Denaturation; Protein Structure, Tertiary | 1993 |
Identification of reactive vicinal cysteines in Saccharomyces cerevisiae (ATP) and cytosolic rat liver (GTP) phospho enol pyruvate carboxykinases.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Cysteine; Cytosol; Dithionitrobenzoic Acid; Fluorescent Dyes; Glucosides; Guanosine Triphosphate; Iodoacetamide; Liver; Phosphoenolpyruvate Carboxykinase (GTP); Pyrimidinones; Rats; Saccharomyces cerevisiae | 1993 |
Disruption of the cysteine-75 and zinc ion coordination is not sufficient to activate the precursor of human matrix metalloproteinase 3 (stromelysin 1).
Topics: Alkylation; Amino Acid Sequence; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Edetic Acid; Enzyme Activation; Humans; Iodoacetamide; Matrix Metalloproteinase 3; Mercuric Chloride; Metalloendopeptidases; Molecular Sequence Data; Phenylmercuric Acetate; Sulfhydryl Reagents; Zinc | 1993 |
Identification of vicinal thiols of phosphoenolpyruvate carboxykinase (GTP).
Topics: Alkylation; Amino Acid Sequence; Animals; Azides; Chromatography, High Pressure Liquid; Cysteine; Cystine; Cytosol; Disulfides; Dithionitrobenzoic Acid; Guanosine Triphosphate; Iodoacetates; Iodoacetic Acid; Liver; Molecular Sequence Data; Peptide Fragments; Phosphoenolpyruvate Carboxykinase (GTP); Rats; Sulfhydryl Compounds; Trypsin | 1993 |
Inhibition of aspartate chemotaxis of Escherichia coli by site-directed sulfhydryl modification of the receptor.
Topics: Aspartic Acid; Bacterial Proteins; Base Sequence; Chemoreceptor Cells; Chemotaxis; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Escherichia coli; Escherichia coli Proteins; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Receptors, Cell Surface; Sulfhydryl Compounds; Threonine | 1993 |
[Directed mutagenesis of bacterial formate dehydrogenase: the role of Cys-255 in the catalysis and stability of the enzyme].
Topics: Base Sequence; Catalysis; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Escherichia coli; Formate Dehydrogenases; Hot Temperature; Mercury; Molecular Sequence Data; Mutagenesis, Site-Directed | 1993 |
Catalytic properties and stability of a Pseudomonas sp.101 formate dehydrogenase mutants containing Cys-255-Ser and Cys-255-Met replacements.
Topics: Amino Acid Sequence; Base Sequence; Catalysis; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Formate Dehydrogenases; Kinetics; Mercury; Methionine; Molecular Sequence Data; Mutation; Oligonucleotides; Pseudomonas; Sequence Homology, Amino Acid; Serine | 1993 |
Staphylococcus aureus alpha-toxin. Production of functionally intact, site-specifically modifiable protein by introduction of cysteine at positions 69, 130, and 186.
Topics: Amino Acid Sequence; Animals; Bacterial Toxins; Chromatography, Ion Exchange; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Hemolysin Proteins; Microscopy, Fluorescence; Mutagenesis, Site-Directed; Rabbits; Recombinant Proteins; Restriction Mapping; Staphylococcus aureus | 1993 |
Comparison of Ellman's reagent with N-(1-pyrenyl)maleimide for the determination of free sulfhydryl groups in reduced cellobiohydrolase I from Trichoderma reesei.
Topics: Cellulose 1,4-beta-Cellobiosidase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Drug Interactions; Fluorescent Dyes; Glycoside Hydrolases; Maleimides; Mercaptoethanol; Oxidation-Reduction; Sulfhydryl Compounds; Trichoderma | 1993 |
Cross-linking and fluorescence study of the COOH- and NH2-terminal domains of intact caldesmon bound to actin.
Topics: Actins; Amino Acid Sequence; Animals; Calmodulin-Binding Proteins; Chickens; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; Gizzard, Avian; Kinetics; Muscle, Skeletal; Muscle, Smooth; Osmolar Concentration; Oxidation-Reduction; Peptide Fragments; Phenanthrolines; Protein Binding; Protein Conformation; Rabbits; Spectrometry, Fluorescence; Thermodynamics | 1995 |
Trypanosoma cruzi phospho enol pyruvate carboxykinase (ATP-dependent): transition metal ion requirement for activity and sulfhydryl group reactivity.
Topics: Adenosine Diphosphate; Animals; Carbon Dioxide; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Enzyme Inhibitors; Kinetics; Magnesium; Maleimides; Manganese; Metals; Phosphoenolpyruvate; Phosphoenolpyruvate Carboxykinase (GTP); Picolinic Acids; Sulfhydryl Compounds; Sulfhydryl Reagents; Trypanosoma cruzi | 1996 |
Synthesis and characterization of a homogeneous chemical conjugate between basic fibroblast growth factor and saporin.
Topics: Amino Acid Sequence; Animals; Base Sequence; Cell Division; Cell Line; Cricetinae; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; DNA Primers; Electrophoresis, Polyacrylamide Gel; Fibroblast Growth Factor 2; Humans; Immunotoxins; Molecular Sequence Data; Mutagenesis, Site-Directed; N-Glycosyl Hydrolases; Plant Proteins; Point Mutation; Protein Biosynthesis; Rats; Recombinant Proteins; Ribosome Inactivating Proteins, Type 1; Ribosomes; Saporins; Succinimides | 1995 |
Site-directed mutagenesis of glutathione synthetase from Escherichia coli B: mapping of the gamma-L-glutamyl-L-cysteine-binding site.
Topics: Base Sequence; Binding Sites; Cysteine; Dipeptides; Dithionitrobenzoic Acid; DNA Primers; Escherichia coli; Glutathione Synthase; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed | 1995 |
The rate of thermal inactivation of Torpedo acetylcholinesterase is not reduced in the C231S mutant.
Topics: Acetylcholinesterase; Animals; Cell Line; Chlorocebus aethiops; Cholinesterase Inhibitors; Cysteine; Dithionitrobenzoic Acid; Hot Temperature; Kinetics; Mutagenesis, Site-Directed; Point Mutation; Recombinant Proteins; Serine; Sulfhydryl Reagents; Thermodynamics; Torpedo; Transfection | 1996 |
Sulfhydryl redox modulates ATP-sensitive K+ channels in rabbit ventricular myocytes.
Topics: Adenosine Triphosphate; Animals; Cells, Cultured; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Glyburide; Heart; Heart Ventricles; Ion Channel Gating; Kinetics; Membrane Potentials; Oxidation-Reduction; Patch-Clamp Techniques; Potassium Channels; Rabbits; Sulfhydryl Reagents; Time Factors | 1996 |
Regulation of HIV-1 protease activity through cysteine modification.
Topics: Catalysis; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Glutathione; HIV Protease; Hydrolysis; Oxidation-Reduction | 1996 |
S-nitrosation of serum albumin: spectrophotometric determination of its nitrosation by simple S-nitrosothiols.
Topics: Animals; Cattle; Cysteine; Dithionitrobenzoic Acid; Glutathione; Nitroso Compounds; S-Nitrosoglutathione; S-Nitrosothiols; Serum Albumin; Spectrophotometry | 1996 |
Reaction of apoflavodoxin from Desulfovibrio vulgaris with 5,5'-dithio-bis-(2-nitrobenzoic acid).
Topics: Apoproteins; Binding Sites; Cysteine; Desulfovibrio vulgaris; Dithionitrobenzoic Acid; Flavin Mononucleotide; Flavodoxin; Kinetics | 1996 |
Cyanylation of the thiol of the cysteine of flavodoxin from Anabaena PCC 7119.
Topics: Anabaena; Apoproteins; Binding Sites; Carbon Isotopes; Cysteine; Dithionitrobenzoic Acid; Flavodoxin; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Potassium Cyanide; Protein Conformation | 1996 |
Murine coronavirus membrane fusion is blocked by modification of thiols buried within the spike protein.
Topics: Animals; Cell Line; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; HeLa Cells; Humans; Membrane Fusion; Membrane Glycoproteins; Mice; Mice, Inbred BALB C; Murine hepatitis virus; Protein Conformation; Rabbits; Receptors, Virus; Spike Glycoprotein, Coronavirus; Structure-Activity Relationship; Sulfhydryl Compounds; Viral Envelope Proteins | 1996 |
On the reactivity and ionization of the active site cysteine residues of Escherichia coli thioredoxin.
Topics: Binding Sites; Cysteine; Disulfides; Dithionitrobenzoic Acid; Escherichia coli; Hydrogen-Ion Concentration; Ions; Models, Chemical; Mutation; Sulfhydryl Compounds; Thioredoxins | 1996 |
Stabilization of barstar by chemical modification of the buried cysteines.
Topics: Bacterial Proteins; Cysteine; Dithionitrobenzoic Acid; Indicators and Reagents; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Denaturation; Protein Folding; Spectrum Analysis; Sulfhydryl Compounds; Thermodynamics; Water | 1996 |
Essential cysteines in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from Escherichia coli: analysis by chemical modification and site-directed mutagenesis.
Topics: Aldehyde-Lyases; Base Sequence; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Inhibitors; Escherichia coli; Methyl Methanesulfonate; Molecular Sequence Data; Pentosephosphates; Phosphoenolpyruvate; Recombinant Proteins; Structure-Activity Relationship; Sulfhydryl Reagents | 1996 |
Evidence for the presence of essential histidine and cysteine residues in platelet cGMP-inhibited phosphodiesterase.
Topics: 3',5'-Cyclic-AMP Phosphodiesterases; Adenosine Monophosphate; Binding Sites; Blood Platelets; Catalysis; Cyclic AMP; Cyclic GMP; Cyclic Nucleotide Phosphodiesterases, Type 3; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Ethylmaleimide; Histidine; Humans; Kinetics; Phosphodiesterase Inhibitors | 1996 |
Variability in heat-induced fragmentation of a protein in the presence of dodecyl sulfate: the role of an intramolecular sulfhydryl/disulfide exchange.
Topics: alpha-Amylases; Amino Acid Sequence; Aspartic Acid; Aspergillus oryzae; Cysteine; Disulfides; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Hot Temperature; Kinetics; Oxidation-Reduction; Peptide Fragments; Protein Denaturation; Proteins; Sodium Dodecyl Sulfate; Sulfhydryl Compounds; Thermodynamics | 1995 |
Probing the active site of Tritrichomonas foetus hypoxanthine-guanine-xanthine phosphoribosyltransferase using covalent modification of cysteine residues.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Iodoacetates; Iodoacetic Acid; Models, Molecular; Molecular Sequence Data; Pentosyltransferases; Sequence Analysis; Sulfhydryl Reagents; Tritrichomonas foetus | 1996 |
Truncations of the C-terminus have different effects on the conformation and activity of phosphatidylinositol transfer protein.
Topics: Acrylamide; Acrylamides; Anilino Naphthalenesulfonates; Animals; Carrier Proteins; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Kinetics; Membrane Proteins; Mutation; Phosphatidylcholines; Phosphatidylinositols; Phospholipid Transfer Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Rats; Recombinant Proteins; Sequence Deletion; Tryptophan | 1996 |
Effect of activation of protein phosphatase 1 on sulfhydryl reactivity.
Topics: Animals; Binding Sites; Cattle; Cobalt; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; In Vitro Techniques; Kinetics; Manganese; Myocardium; Phosphoprotein Phosphatases; Protein Conformation; Protein Denaturation; Protein Phosphatase 1; Recombinant Proteins; Sulfhydryl Reagents; Thiocyanates | 1996 |
Reactivity of essential cysteine and lysine residues present at the catalytic domain of pig heart mitochondrial malate dehydrogenase.
Topics: Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Inhibitors; Hydrogen-Ion Concentration; Kinetics; Lysine; Malate Dehydrogenase; Mitochondria, Heart; NAD; o-Phthalaldehyde; Pyridoxal Phosphate; Swine | 1995 |
Structural characterization of the metal binding site in the cysteine-rich region of HIV-1 Tat protein.
Topics: Amino Acid Sequence; Binding Sites; Cations, Divalent; Circular Dichroism; Cysteine; Disulfides; Dithionitrobenzoic Acid; Gene Products, tat; HIV-1; Mass Spectrometry; Models, Structural; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Spectrophotometry, Ultraviolet; tat Gene Products, Human Immunodeficiency Virus; Zinc | 1996 |
Role of the cysteine residues in the alpha1,2-mannosidase involved in N-glycan biosynthesis in Saccharomyces cerevisiae. The conserved Cys340 and Cys385 residues form an essential disulfide bond.
Topics: Amino Acid Sequence; Animals; Binding Sites; Carbohydrate Sequence; Chromatography, High Pressure Liquid; Conserved Sequence; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Drosophila; Humans; Mannosidases; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Peptide Mapping; Polymerase Chain Reaction; Polysaccharides; Rabbits; Recombinant Proteins; Saccharomyces cerevisiae; Sequence Homology, Amino Acid | 1996 |
Speciation and quantitation of underivatized and Ellman's derivatized biological thiols and disulfides by capillary electrophoresis.
Topics: Captopril; Cysteine; Disulfides; Dithionitrobenzoic Acid; Electrophoresis, Capillary; Erythrocytes; Glutathione; Humans; Sensitivity and Specificity; Sulfhydryl Compounds | 1996 |
Effects of reducing agents and oxidants on excitation-contraction coupling in skeletal muscle fibres of rat and toad.
Topics: 2,2'-Dipyridyl; Animals; Bufo marinus; Calcium; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; In Vitro Techniques; Membrane Potentials; Muscle Contraction; Muscle Fibers, Skeletal; Muscle, Skeletal; Oxidants; Rats; Reducing Agents; Sarcoplasmic Reticulum; Sulfhydryl Reagents | 1996 |
Identification of sulfhydryl-modified cysteine residues in the ligand binding pocket of retinoic acid receptor beta.
Topics: Binding Sites; Blotting, Western; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Mutagenesis, Site-Directed; Plasmids; Polymerase Chain Reaction; Protease Inhibitors; Receptors, Retinoic Acid; Retinoic Acid Receptor alpha; Retinoic Acid Receptor gamma; Structure-Activity Relationship; Sulfhydryl Reagents; Tretinoin | 1997 |
Identification of an essential cysteine residue in human glutathione synthase.
Topics: Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Glutathione Synthase; Humans; Iodoacetates; Iodoacetic Acid; Mutagenesis, Site-Directed; Phencyclidine | 1997 |
Destabilizing interactions between the partners of a bifunctional fusion protein.
Topics: ATP-Binding Cassette Transporters; Bacteriophage M13; Carrier Proteins; Chromatography, Gel; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Guanidine; Guanidines; Kinetics; Maltose-Binding Proteins; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Mutation; Oxidation-Reduction; Periplasmic Binding Proteins; Protein Denaturation; Recombinant Fusion Proteins; Ultracentrifugation | 1996 |
Loss of GSH, oxidative stress, and decrease of intracellular pH as sequential steps in viral infection.
Topics: Adsorption; Animals; Buthionine Sulfoximine; Cell Line; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Dogs; Glutathione; Glutathione Disulfide; Hydrogen-Ion Concentration; Kidney; L-Lactate Dehydrogenase; Methionine; Oxidative Stress; Respirovirus; Sodium-Hydrogen Exchangers; Ultraviolet Rays; Virus Replication | 1997 |
Fluorescent probes attached to Cys 35 or Cys 84 in cardiac troponin C are differentially sensitive to Ca(2+)-dependent events in vitro and in situ.
Topics: Amino Acid Sequence; Animals; Binding Sites; Calcium; Chickens; Cysteine; Dithionitrobenzoic Acid; Fluorescent Dyes; In Vitro Techniques; Kinetics; Molecular Sequence Data; Molecular Structure; Naphthalenesulfonates; Point Mutation; Recombinant Proteins; Sulfhydryl Reagents; Troponin C | 1997 |
Cysteine 265 is in the active site of, but is not essential for catalysis by tRNA-guanine transglycosylase (TGT) from Escherichia coli.
Topics: Binding Sites; Catalysis; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Escherichia coli; Kinetics; Mutation; Pentosyltransferases; Titrimetry | 1997 |
Carbonyl reducing 17 beta-hydroxysteroid dehydrogenase from the filamentous fungus Cochliobolus lunatus.
Topics: Alcohol Oxidoreductases; Aldehyde Reductase; Aldo-Keto Reductases; Androstenedione; Ascomycota; Binding Sites; Binding, Competitive; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Histidine; Hydroxysteroid Dehydrogenases; Immunoblotting; Kinetics; Lysine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet; Tetranitromethane; Trinitrobenzenesulfonic Acid; Tyrosine; Vitamin K | 1997 |
Chemical modification and site-directed mutagenesis of the single cysteine in motif 3 of class II Escherichia coli prolyl-tRNA synthetase.
Topics: Amino Acid Sequence; Amino Acyl-tRNA Synthetases; Binding Sites; Catalysis; Cysteine; Dithionitrobenzoic Acid; Enzyme Inhibitors; Escherichia coli; Iodoacetamide; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Naphthalenesulfonates; Point Mutation; RNA, Transfer, Amino Acyl; Sequence Alignment | 1997 |
The sulfhydryl groups of Cys 269 and Cys 272 are critical for the oligomeric state of chloroplast carbonic anhydrase from Pisum sativum.
Topics: Carbon Dioxide; Carbonic Anhydrase Inhibitors; Carbonic Anhydrases; Chloroplasts; Circular Dichroism; Cross-Linking Reagents; Cysteine; Diamide; Dithionitrobenzoic Acid; Escherichia coli; Ethoxzolamide; Gene Expression; Kinetics; Molecular Weight; Mutagenesis, Site-Directed; Phosphines; Pisum sativum; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence | 1997 |
Quantification and location of disulfide bonds in proteins.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Electrophoresis; Peptides; Proteins; Spectrometry, Mass, Fast Atom Bombardment | 1997 |
Effect of N-B transition on the microenvironment surrounding 34Cys in human serum albumin.
Topics: 2-Naphthylamine; Cysteine; Dithionitrobenzoic Acid; Fluorescent Dyes; Humans; Hydrogen-Ion Concentration; Kinetics; Oleic Acid; Serum Albumin; Spectrometry, Fluorescence | 1997 |
Effects of amino acid replacements on cadmium binding of metallothionein alpha-fragment.
Topics: Amino Acid Sequence; Cadmium; Cysteine; Dithionitrobenzoic Acid; Gene Expression; Genetic Vectors; Humans; Metallothionein; Molecular Sequence Data; Recombinant Proteins; Structure-Activity Relationship | 1997 |
Unexpected lability of cysteine acetamidomethyl thiol protecting group. Tyrosine ring alkylation and disulfide bond formation upon acidolysis.
Topics: Alkylation; Amino Acid Sequence; Cysteine; Disulfides; Dithionitrobenzoic Acid; Drug Stability; Oligopeptides; Peptides, Cyclic; Resins, Plant; Sulfhydryl Compounds; Tyrosine | 1997 |
Modification of Cys-837 identifies an actin-binding site in the beta-propeller protein scruin.
Topics: Actins; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Horseshoe Crabs; Male; Models, Molecular; Peptides; Protein Binding; Protein Structure, Secondary; Spermatozoa; Structure-Activity Relationship; Titrimetry | 1997 |
Possible thiol group involvement in intracellular pH effect on low-conductance Ca(2+)-dependent K+ channels.
Topics: Calcium; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Electric Stimulation; Ethylmaleimide; HeLa Cells; Humans; Hydrogen-Ion Concentration; Intracellular Fluid; Ion Channel Gating; Kinetics; Membrane Potentials; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Probability; Sulfhydryl Reagents; Time Factors | 1997 |
Active site titration of the tyrosine phosphatases SHP-1 and PTP1B using aromatic disulfides. Reaction with the essential cysteine residue in the active site.
Topics: Binding Sites; Cysteine; Disulfides; Dithionitrobenzoic Acid; Intracellular Signaling Peptides and Proteins; Kinetics; Molecular Probes; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Protein Tyrosine Phosphatase, Non-Receptor Type 6; Protein Tyrosine Phosphatases | 1997 |
Determination of glutathione, cysteine and N-acetylcysteine in rabbit eye tissues using high-performance liquid chromatography and post-column derivatization with 5,5'-dithiobis(2-nitrobenzoic acid).
Topics: Acetylcysteine; Animals; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Eye; Glutathione; Hydrogen-Ion Concentration; Lens, Crystalline; Rabbits; Reference Values; Retina; Spectrophotometry, Ultraviolet; Sulfhydryl Reagents; Vitrectomy; Vitreous Body | 1997 |
Mass spectrometric determination of the cleavage sites in Escherichia coli dihydroorotase induced by a cysteine-specific reagent.
Topics: Binding Sites; Cysteine; Dihydroorotase; Dithionitrobenzoic Acid; Escherichia coli; Guanidine; Kinetics; Macromolecular Substances; Peptide Fragments; Spectrometry, Mass, Secondary Ion; Sulfhydryl Reagents; Thiocyanates | 1997 |
Roles for the two cysteine residues of AhpC in catalysis of peroxide reduction by alkyl hydroperoxide reductase from Salmonella typhimurium.
Topics: Bacterial Proteins; Catalysis; Cysteine; Disulfides; Dithionitrobenzoic Acid; Hydrogen Peroxide; Kinetics; Mutagenesis, Site-Directed; NAD; Nitrobenzoates; Oxidation-Reduction; Oxidoreductases; Peroxidases; Peroxiredoxins; Salmonella typhimurium; Sulfhydryl Compounds; Titrimetry | 1997 |
Requirement for the two AhpF cystine disulfide centers in catalysis of peroxide reduction by alkyl hydroperoxide reductase.
Topics: Bacterial Proteins; Catalysis; Circular Dichroism; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Escherichia coli Proteins; Flavins; Mutagenesis, Site-Directed; NAD; Oxidation-Reduction; Oxidoreductases; Peroxidases; Peroxides; Peroxiredoxins; Salmonella typhimurium; Spectrometry, Fluorescence; Titrimetry | 1997 |
Cancer-preventive selenocompounds induce a specific redox modification of cysteine-rich regions in Ca(2+)-dependent isoenzymes of protein kinase C.
Topics: Animals; Anticarcinogenic Agents; Binding Sites; Brain; Calcium; Cyclic AMP-Dependent Protein Kinases; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; Isoenzymes; Kinetics; Nitrobenzoates; Organoselenium Compounds; Oxidation-Reduction; Phosphoprotein Phosphatases; Phosphorylase Kinase; Protein Kinase C; Protein Kinase C beta; Protein Kinase C-alpha; Protein Kinase C-delta; Protein Phosphatase 2; Rats; Sodium Selenite | 1997 |
Mutational analysis of the three cysteines and active-site aspartic acid 103 of ketosteroid isomerase from Pseudomonas putida biotype B.
Topics: Aspartic Acid; Binding Sites; Cysteine; Dithionitrobenzoic Acid; DNA Mutational Analysis; Enzyme Stability; Equilenin; Hydrogen Bonding; Ketosteroids; Kinetics; Models, Molecular; Pseudomonas putida; Recombinant Proteins; Spectrophotometry, Ultraviolet; Steroid Isomerases; Titrimetry | 1997 |
Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis.
Topics: Alkylating Agents; Binding Sites; Cysteine; Dihydroorotate Dehydrogenase; Dithionitrobenzoic Acid; Enzyme Inhibitors; Evolution, Molecular; Flavoproteins; Hydrogen-Ion Concentration; Kinetics; Lactococcus lactis; Mutagenesis, Site-Directed; Orotic Acid; Oxidation-Reduction; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Spectrophotometry | 1997 |
Differential modulation of the uptake currents by redox interconversion of cysteine residues in the human neuronal glutamate transporter EAAC1.
Topics: Amino Acid Transport System X-AG; Animals; Carrier Proteins; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Evoked Potentials; Excitatory Amino Acid Transporter 3; Female; Glutamate Plasma Membrane Transport Proteins; Glutamates; Humans; Neurons; Nitrates; Oocytes; Oxidants; Oxidation-Reduction; Patch-Clamp Techniques; Symporters; Xenopus | 1997 |
Effect of selective cysteine --> alanine replacements on the catalytic functions of lysine: N6-hydroxylase.
Topics: 2,6-Dichloroindophenol; Alanine; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Flavoproteins; Iodoacetates; Iodoacetic Acid; Lysine; Mass Spectrometry; Mixed Function Oxygenases; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP; Peptide Fragments; Protein Conformation; Recombinant Proteins; Sequence Analysis; Trypsin | 1997 |
Characterization of a mammalian peroxiredoxin that contains one conserved cysteine.
Topics: Amino Acid Sequence; Conserved Sequence; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione Peroxidase; Humans; Hydrogen Peroxide; Models, Chemical; Molecular Sequence Data; Neoplasm Proteins; Oxidation-Reduction; Peroxidases; Peroxiredoxin III; Peroxiredoxins; Phospholipases A; Phospholipases A2; Proteins; Recombinant Proteins; Selenocysteine; Sequence Homology, Amino Acid; Subcellular Fractions | 1998 |
The switch in the helical handedness of the histone (H3-H4)2 tetramer within a nucleoprotein particle requires a reorientation of the H3-H3 interface.
Topics: Animals; Base Sequence; Cysteine; Dithionitrobenzoic Acid; DNA; DNA Primers; DNA, Circular; Ducks; Ethylmaleimide; Histones; Iodoacetamide; Kinetics; Macromolecular Substances; Mammary Tumor Virus, Mouse; Nucleoproteins; Protein Structure, Secondary; Repetitive Sequences, Nucleic Acid; Stress, Mechanical; Sulfhydryl Reagents | 1998 |
Reduction-oxidation (Redox) and vascular tissue level of homocyst(e)ine in human coronary atherosclerotic lesions and role in extracellular matrix remodeling and vascular tone.
Topics: Arteries; Cell Extracts; Coronary Artery Disease; Coronary Vessels; Culture Techniques; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Endothelium, Vascular; Extracellular Matrix; Homocysteine; Homocystine; Humans; Isometric Contraction; Metalloendopeptidases; Oxidation-Reduction; Vasoconstriction | 1998 |
Solubilization of hydrophobic peptides by reversible cysteine PEGylation.
Topics: Amino Acid Sequence; Carboxypeptidase B; Carboxypeptidases; Circular Dichroism; Cysteine; Disulfides; Dithionitrobenzoic Acid; Molecular Conformation; Molecular Sequence Data; Molecular Structure; Peptides; Phosphines; Polyethylene Glycols; Solubility | 1998 |
Status of Cys residues in the covalent structure of botulinum neurotoxin types A, B, and E.
Topics: Botulinum Toxins; Botulinum Toxins, Type A; Clostridium botulinum; Cysteine; Disulfides; Dithionitrobenzoic Acid; Protein Conformation | 1998 |
Sulfhydryl reagent susceptibility in proteins with high sequence similarity--triosephosphate isomerase from Trypanosoma brucei, Trypanosoma cruzi and Leishmania mexicana.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Kinetics; Leishmania mexicana; Methyl Methanesulfonate; Mutagenesis, Site-Directed; Polymerase Chain Reaction; Protein Conformation; Recombinant Proteins; Sulfhydryl Reagents; Triose-Phosphate Isomerase; Trypanosoma brucei brucei; Trypanosoma cruzi | 1998 |
Formation and properties of mixed disulfides between thioredoxin reductase from Escherichia coli and thioredoxin: evidence that cysteine-138 functions to initiate dithiol-disulfide interchange and to accept the reducing equivalent from reduced flavin.
Topics: Cysteine; Disulfides; Dithionite; Dithionitrobenzoic Acid; Dithiothreitol; Escherichia coli; Flavin-Adenine Dinucleotide; Hydrogen-Ion Concentration; Mutagenesis, Site-Directed; NADP; Osmolar Concentration; Oxidation-Reduction; Protein Conformation; Protein Engineering; Spectrometry, Fluorescence; Structure-Activity Relationship; Sulfhydryl Compounds; Thioredoxin-Disulfide Reductase; Thioredoxins | 1998 |
Genetically engineered zinc-chelating adenylate kinase from Escherichia coli with enhanced thermal stability.
Topics: Adenylate Kinase; Amino Acid Sequence; Amino Acid Substitution; Base Sequence; Catalysis; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Escherichia coli; Genetic Engineering; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Spectrophotometry, Atomic; Temperature; Trypsin; Zinc | 1998 |
Fast-reacting thiols in rat hemoglobins can intercept damaging species in erythrocytes more efficiently than glutathione.
Topics: Animals; Cysteine; Diamide; Disulfides; Dithionitrobenzoic Acid; Erythrocytes; Glutathione; Hemoglobins; Male; Models, Molecular; Oxidants; Oxidative Stress; Protein Conformation; Rats; Rats, Sprague-Dawley; Structure-Activity Relationship; Sulfhydryl Compounds; Sulfhydryl Reagents | 1998 |
Changes in side chain packing during apomyoglobin folding characterized by pulsed thiol-disulfide exchange.
Topics: Amino Acids, Sulfur; Apoproteins; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Flow Injection Analysis; Kinetics; Methyl Methanesulfonate; Models, Chemical; Models, Molecular; Mutagenesis, Site-Directed; Myoglobin; Protein Denaturation; Protein Engineering; Protein Folding; Spectrometry, Fluorescence; Sulfhydryl Compounds; Sulfhydryl Reagents | 1998 |
Cysteine reactivity and oligomeric structures of phospholamban and its mutants.
Topics: Animals; Calcium-Binding Proteins; Calcium-Transporting ATPases; Cysteine; Dithionitrobenzoic Acid; Electron Spin Resonance Spectroscopy; Electrophoresis, Polyacrylamide Gel; Isoleucine; Leucine; Mesylates; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Spin Labels; Spodoptera; Sulfhydryl Compounds | 1998 |
Spectroscopic study of Y210C lambda-repressor: implications for cooperative interaction.
Topics: Bacteriophage lambda; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; DNA; DNA-Binding Proteins; Fluorescence Polarization; Macromolecular Substances; Models, Molecular; Mutagenesis; Operon; Protein Conformation; Repressor Proteins; Spectrometry, Fluorescence; Spectrophotometry; Sulfhydryl Reagents; Viral Proteins; Viral Regulatory and Accessory Proteins | 1998 |
Probing the role of cysteine residues in the EcoP15I DNA methyltransferase.
Topics: Amino Acid Substitution; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; DNA Methylation; Escherichia coli; Ethylmaleimide; Iodoacetamide; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Recombinant Proteins; Site-Specific DNA-Methyltransferase (Adenine-Specific) | 1998 |
Exploring a channel to the active site of copper/topaquinone-containing phenylethylamine oxidase by chemical modification and site-specific mutagenesis.
Topics: 4-Chloro-7-nitrobenzofurazan; Amine Oxidase (Copper-Containing); Amino Acid Sequence; Apoenzymes; Binding Sites; Copper; Cysteine; Dihydroxyphenylalanine; Dithionitrobenzoic Acid; Fluorescent Dyes; Histidine; Holoenzymes; Lysine; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Substrate Specificity | 1998 |
Nitric oxide inhibition of the rat olfactory cyclic nucleotide-gated cation channel.
Topics: Animals; Cyclic AMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; Ion Channel Gating; Ion Channels; Molsidomine; Nitric Oxide Donors; Nitroso Compounds; Olfactory Receptor Neurons; Rats; S-Nitrosothiols; Sulfhydryl Reagents | 1998 |
Rotation of the epsilon subunit during catalysis by Escherichia coli FOF1-ATP synthase.
Topics: Catalysis; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Kinetics; Macromolecular Substances; Mutagenesis, Site-Directed; Protein Multimerization; Proton-Translocating ATPases; Recombinant Proteins; Rotation | 1998 |
pH-dependent gating of ROMK (Kir1.1) channels involves conformational changes in both N and C termini.
Topics: Amino Acid Substitution; Animals; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Female; Hydrogen-Ion Concentration; In Vitro Techniques; Ion Channel Gating; Kidney; Membrane Potentials; Mutagenesis, Site-Directed; Oocytes; Oxidation-Reduction; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Protein Conformation; Recombinant Proteins; Xenopus laevis | 1998 |
Influence of chemical modification of cysteine and histidine side chains upon subunit reassembly of alpha crystallin.
Topics: Chromatography, Gel; Circular Dichroism; Crystallins; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Histidine; Protein Conformation; Protein Folding; Structure-Activity Relationship | 1998 |
Identification of two electron-transfer sites in ascorbate peroxidase using chemical modification, enzyme kinetics, and crystallography.
Topics: Ascorbate Peroxidases; Binding Sites; Catalysis; Crystallization; Crystallography, X-Ray; Cysteine; Dithionitrobenzoic Acid; Electron Transport; Enzyme Activation; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Osmolar Concentration; Peroxidases; Spectrophotometry, Ultraviolet; Spectrum Analysis; Substrate Specificity | 1998 |
Metal chelating properties of adenylate kinase from Paracoccus denitrificans.
Topics: Adenylate Kinase; Amino Acid Sequence; Base Sequence; Chelating Agents; Cloning, Molecular; Cysteine; Dimerization; Dithionitrobenzoic Acid; Enzyme Stability; Escherichia coli; Histidine; Iron; Kinetics; Molecular Sequence Data; Oxidation-Reduction; Paracoccus denitrificans; Phosphorylation; Protein Denaturation; Recombinant Proteins; Sequence Homology, Amino Acid; Titrimetry; Trypsin; Zinc | 1998 |
Inactivation of the purified bovine mu opioid receptor by sulfhydryl reagents.
Topics: Animals; Benzomorphans; Cattle; Cell Membrane; Corpus Striatum; Cysteine; Dithionitrobenzoic Acid; Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; Enkephalins; Ethylmaleimide; Kinetics; Ligands; Naloxone; Radioligand Assay; Receptors, Opioid, mu; Sulfhydryl Reagents; Tritium | 1999 |
The internal Cys-207 of sorghum leaf NADP-malate dehydrogenase can form mixed disulphides with thioredoxin.
Topics: Animals; Chlamydomonas; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithionitrobenzoic Acid; Enzyme Activation; Kinetics; Malate Dehydrogenase; Malate Dehydrogenase (NADP+); Mutagenesis, Site-Directed; Plant Leaves; Plant Proteins; Recombinant Proteins; Thioredoxins | 1999 |
Thioredoxin reductase from Plasmodium falciparum: evidence for interaction between the C-terminal cysteine residues and the active site disulfide-dithiol.
Topics: Alanine; Animals; Binding Sites; Cysteine; Disulfides; Dithionite; Dithionitrobenzoic Acid; Kinetics; Mutagenesis, Site-Directed; NADP; Oxidation-Reduction; Peptide Fragments; Plasmodium falciparum; Thioredoxin-Disulfide Reductase; Titrimetry; Toluene | 1999 |
Derivatization of the interface cysteine of triosephosphate isomerase from Trypanosoma brucei and Trypanosoma cruzi as probe of the interrelationship between the catalytic sites and the dimer interface.
Topics: Animals; Catalysis; Cysteine; Dimerization; Dithionitrobenzoic Acid; Enzyme Activation; Enzyme Inhibitors; Glyceraldehyde 3-Phosphate; Glycolates; Methyl Methanesulfonate; Mutagenesis, Site-Directed; Serine; Substrate Specificity; Sulfhydryl Reagents; Triose-Phosphate Isomerase; Trypanosoma brucei brucei; Trypanosoma cruzi | 1999 |
Light-dependent changes in redox status of the plastidic acetyl-CoA carboxylase and its regulatory component.
Topics: Acetyl-CoA Carboxylase; Carbon-Nitrogen Ligases; Carboxyl and Carbamoyl Transferases; Carrier Proteins; Cell Extracts; Chloroplasts; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Fatty Acid Synthase, Type II; Hydrogen-Ion Concentration; Light; Malonyl Coenzyme A; Multienzyme Complexes; Oxidation-Reduction; Pisum sativum; Plant Leaves; Time Factors | 1999 |
Evidence that lipid hydroperoxides inhibit plasma lecithin:cholesterol acyltransferase activity.
Topics: Arteriosclerosis; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Inhibitors; Humans; In Vitro Techniques; Lipid Peroxides; Lipoproteins, LDL; Lipoxygenase; Phosphatidylcholine-Sterol O-Acyltransferase | 1999 |
Regulation of PTP1B via glutathionylation of the active site cysteine 215.
Topics: Amino Acid Sequence; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Glutathione; Glutathione Disulfide; Humans; Kinetics; Mass Spectrometry; Molecular Sequence Data; Oxidation-Reduction; Protein Tyrosine Phosphatases; Titrimetry | 1999 |
Evidence that cysteine-166 is the active-site nucleophile of Pseudomonas aeruginosa amidase: crystallization and preliminary X-ray diffraction analysis of the enzyme.
Topics: Amidohydrolases; Amino Acid Substitution; Binding Sites; Circular Dichroism; Cross Reactions; Crystallization; Crystallography, X-Ray; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Immunodiffusion; Molecular Weight; Protein Conformation; Protein Folding; Pseudomonas aeruginosa; Recombinant Proteins; Sulfhydryl Compounds | 1999 |
Effect of cysteine residues on the activity of arginyl-tRNA synthetase from Escherichia coli.
Topics: Acylation; Arginine-tRNA Ligase; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Escherichia coli; Ethylmaleimide; Gene Expression Regulation, Enzymologic; Iodoacetamide; Kinetics; Mutagenesis, Site-Directed; Recombinant Proteins; Sulfhydryl Reagents; Titrimetry | 1999 |
5,5'-Dithiobis-(2-nitrobenzoic acid) as a probe for a non-essential cysteine residue at the medium chain acyl-coenzyme A dehydrogenase binding site of the human 'electron transferring flavoprotein' (ETF).
Topics: Acyl-CoA Dehydrogenase; Acyl-CoA Dehydrogenases; Binding Sites; Biomarkers; Cysteine; Dithionitrobenzoic Acid; Electron Transport; Electron-Transferring Flavoproteins; Flavoproteins; Humans; Kinetics; Molecular Probes; Protein Binding; Sulfhydryl Reagents | 1999 |
Intrabody construction and expression. I. The critical role of VL domain stability.
Topics: Amino Acid Sequence; Animals; Consensus Sequence; Cysteine; Cytoplasm; Disulfides; Dithionitrobenzoic Acid; Escherichia coli; Gene Expression; Hydrogen-Ion Concentration; Immunoglobulin Light Chains; Immunoglobulin Variable Region; Methionine; Mice; Oxidation-Reduction; Point Mutation; Promoter Regions, Genetic; Protein Engineering; Protein Folding; Recombinant Proteins; Solubility; Thermodynamics | 1999 |
A critical role of the N-methyl-D-aspartate (NMDA) receptor subunit (NR) 2A in the expression of redox sensitivity of NR1/NR2A recombinant NMDA receptors.
Topics: Animals; Binding Sites; CHO Cells; Cricetinae; Cysteine; Dithionitrobenzoic Acid; Mutation; Oxidation-Reduction; Receptors, N-Methyl-D-Aspartate; Recombinant Proteins; Reducing Agents; Sulfhydryl Reagents; Time Factors; Transfection | 1999 |
Involvement of cysteine residues and domain interactions in the reversible unfolding of lipoxygenase-1.
Topics: Calorimetry; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Glycine max; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Lipoxygenase; Protein Conformation; Protein Denaturation; Protein Folding; Thermodynamics; Urea | 1999 |
Oxidation and reduction of pig skeletal muscle ryanodine receptors.
Topics: Animals; Biophysical Phenomena; Biophysics; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; In Vitro Techniques; Malignant Hyperthermia; Muscle, Skeletal; Myocardium; Oxidation-Reduction; Point Mutation; Pyridines; Ryanodine Receptor Calcium Release Channel; Sulfhydryl Reagents; Swine; Swine Diseases | 1999 |
Nucleosome dynamics V. Ethidium bromide versus histone tails in modulating ethidium bromide-driven tetrasome chiral transition. A fluorescence study of tetrasomes on DNA minicircles.
Topics: Acetylation; Binding Sites; Cysteine; Dimerization; Dithionitrobenzoic Acid; DNA, Circular; DNA, Superhelical; Ethidium; Fluorescence; Histones; Intercalating Agents; Molecular Weight; Nucleic Acid Conformation; Nucleosomes; Osmolar Concentration; Pliability; Stereoisomerism; Temperature; Thermodynamics; Titrimetry | 2000 |
Thiolation of low-Mr phosphotyrosine protein phosphatase by thiol-disulfides.
Topics: Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Glutathione Disulfide; Humans; Isoenzymes; Kinetics; Molecular Weight; Phosphates; Protein Tyrosine Phosphatases; Sulfhydryl Reagents | 1999 |
Measurements of cysteine reactivity during protein unfolding suggest the presence of competing pathways.
Topics: Amino Acid Substitution; Bacterial Proteins; Binding, Competitive; Cysteine; Dithionitrobenzoic Acid; Fluorescence; Hydrogen; Hydrogen-Ion Concentration; Kinetics; Mathematics; Models, Chemical; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Thermodynamics | 2000 |
Thiol modification of diacylglycerol kinase: dependence upon site membrane disposition and reagent hydrophobicity.
Topics: Amino Acid Sequence; Cysteine; Diacylglycerol Kinase; Disulfides; Dithionitrobenzoic Acid; Molecular Sequence Data; Sulfhydryl Compounds | 2000 |
Status of glutathione and other thiols and disulfides in human plasma.
Topics: Adult; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithionitrobenzoic Acid; Glutathione; Homocysteine; Humans; In Vitro Techniques; Male; Oxidation-Reduction; Sulfhydryl Compounds; Sulfhydryl Reagents | 2000 |
Sulfhydryls of tubulin. A probe to detect conformational changes of tubulin.
Topics: Anilino Naphthalenesulfonates; Animals; Colchicine; Cysteine; Dithionitrobenzoic Acid; Protein Conformation; Rabbits; Sulfhydryl Compounds; Sulfhydryl Reagents; Time Factors; Tropolone; Tubulin | 2000 |
Minor thiols cysteine and cysteinylglycine regulate the competition between glutathione and protein SH groups in human platelets subjected to oxidative stress.
Topics: Antioxidants; Blood Platelets; Chromatography, High Pressure Liquid; Cysteine; Diamide; Dipeptides; Disulfides; Dithionitrobenzoic Acid; Erythrocytes; Glutathione; Humans; Oxidative Stress; Spectrophotometry; src Homology Domains; Sulfhydryl Compounds; tert-Butylhydroperoxide; Time Factors | 2000 |
Inhibition of glutamate uptake by Tx3-4 is dependent on the redox state of cysteine residues.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Biological Transport; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Glutamic Acid; Hippocampus; Neuropeptides; Neurotoxins; Oxidation-Reduction; Rats; Rats, Wistar; Spider Venoms; Synaptosomes | 2000 |
Microenvironment of cysteine 242 in type-1 ribosome-inactivating protein from iris.
Topics: Amino Acid Sequence; Cysteine; Dithionitrobenzoic Acid; Models, Molecular; N-Glycosyl Hydrolases; Plant Proteins; Plants; Protein Conformation; Ribosome Inactivating Proteins; Spectrometry, Fluorescence | 2000 |
A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli.
Topics: Binding Sites; Catalysis; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Escherichia coli; Methionine; Methionine Sulfoxide Reductases; Models, Chemical; Molecular Weight; Mutation; Oxidoreductases; Peptides; Reducing Agents; Spectrometry, Mass, Electrospray Ionization; Sulfenic Acids; Sulfhydryl Compounds; Thioredoxins | 2000 |
Effects of bienzyme complex formation of cysteine synthetase from escherichia coli on some properties and kinetics.
Topics: Acetyltransferases; Cysteine; Cysteine Synthase; Dithionitrobenzoic Acid; Escherichia coli; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Serine; Serine O-Acetyltransferase | 2000 |
The role of cysteine residues in tellurite resistance mediated by the TehAB determinant.
Topics: Alanine; Alkylating Agents; Bacterial Proteins; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Drug Resistance; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Iodoacetamide; Iodoacetates; Ligands; Microbial Sensitivity Tests; Mutagenesis, Site-Directed; Phenotype; Plasmids; Protein Binding; Protein Conformation; Protein Denaturation; S-Adenosylmethionine; Sulfhydryl Reagents; Tellurium; Up-Regulation | 2000 |
Functional characterization of zinc-finger motif in redox regulation of RPA-ssDNA interaction.
Topics: Biosensing Techniques; Chelating Agents; Cysteine; Dithionitrobenzoic Acid; DNA Replication; DNA-Binding Proteins; DNA, Single-Stranded; Macromolecular Substances; Oxidation-Reduction; Phenanthrolines; Replication Protein A; Zinc; Zinc Fingers | 2000 |
Importance of the free sulfhydryl groups of lecithin-cholesterol acyltransferase for its sensitivity to oxidative inactivation.
Topics: Animals; Binding Sites; Chickens; CHO Cells; Cricetinae; Cysteine; Dithionitrobenzoic Acid; Glycine; Humans; Mutagenesis, Site-Directed; Mutation; Oxidative Stress; Phosphatidylcholine-Sterol O-Acyltransferase; Plasmids; Substrate Specificity; Sulfhydryl Compounds; Transfection | 2000 |
Different metabolizing ability of thiol reactants in human and rat blood: biochemical and pharmacological implications.
Topics: Adult; Animals; Chromatography, High Pressure Liquid; Cysteine; Diamide; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Erythrocytes; Glutathione; Glutathione Reductase; Hemoglobins; Humans; Hydrogen Peroxide; Male; Middle Aged; Nitric Oxide; Nitric Oxide Donors; Nitroso Compounds; Rats; Rats, Wistar; S-Nitrosothiols; Sulfhydryl Compounds; Sulfhydryl Reagents; Time Factors | 2001 |
Direct demonstration that homotetrameric chaperone SecB undergoes a dynamic dimer-tetramer equilibrium.
Topics: Bacterial Proteins; Chromatography; Chromatography, High Pressure Liquid; Cysteine; Cytosol; Dimerization; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Models, Biological; Protein Binding; Protein Folding; Sulfhydryl Reagents | 2001 |
Construction, separation and properties of hybrid hexamers of glutamate dehydrogenase in which five of the six subunits are contributed by the catalytically inert D165S.
Topics: Allosteric Regulation; Allosteric Site; Amino Acid Substitution; Catalysis; Chromatography, Affinity; Clostridium; Coloring Agents; Cysteine; Dithionitrobenzoic Acid; Glutamate Dehydrogenase; Glutamic Acid; Hydrogen-Ion Concentration; Kinetics; Ligands; Mutation; NAD; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Quaternary; Protein Subunits; Recombinant Fusion Proteins; Triazines; Ultrafiltration; Urea | 2001 |
Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activity.
Topics: 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; 2',3'-Cyclic-Nucleotide Phosphodiesterases; Amino Acid Sequence; Animals; Base Sequence; Catalytic Domain; Cysteine; Dithionitrobenzoic Acid; DNA Primers; Enzyme Inhibitors; Histidine; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphoric Diester Hydrolases; Rats; Recombinant Proteins; Sequence Homology, Amino Acid | 2001 |
Structural and functional consequences of inactivation of human glutathione S-transferase P1-1 mediated by the catechol metabolite of equine estrogens, 4-hydroxyequilenin.
Topics: Amino Acid Substitution; Animals; Cysteine; Disulfides; Dithionitrobenzoic Acid; Electron Spin Resonance Spectroscopy; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Inhibitors; Equilenin; Estrogens, Catechol; Free Radical Scavengers; Glutathione S-Transferase pi; Glutathione Transferase; Histidine; Horses; Humans; Isoenzymes; Kinetics; Mutagenesis, Site-Directed; Oxidation-Reduction; Recombinant Proteins; Reducing Agents; Structure-Activity Relationship; Sulfhydryl Compounds; Sulfhydryl Reagents; Titrimetry | 2001 |
Pressure-induced perturbation on the active site of beta-amylase monitored from the sulfhydryl reaction.
Topics: beta-Amylase; Binding Sites; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Guanidine; Pressure; Protein Conformation; Protein Folding; Solanaceae; Spectrometry, Fluorescence; Sulfhydryl Reagents; Thermodynamics; Thiocyanates | 2001 |
Conformation and polarity of the active site of xylanase I from Thermomonospora sp. as deduced by fluorescent chemoaffinity labeling. Site and significance of a histidine residue.
Topics: Actinomycetales; Affinity Labels; Amino Acids; Binding Sites; Circular Dichroism; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Enzyme Activation; Ethylmaleimide; Histidine; Hydroxymercuribenzoates; Indicators and Reagents; Kinetics; Lysine; Models, Chemical; o-Phthalaldehyde; Protease Inhibitors; Protein Binding; Protein Conformation; Sequence Analysis, Protein; Spectrometry, Fluorescence; Sulfhydryl Reagents; Time Factors; Trinitrobenzenesulfonic Acid; Urea; Xylan Endo-1,3-beta-Xylosidase; Xylosidases | 2001 |
The role of the cysteine residues of ThiI in the generation of 4-thiouridine in tRNA.
Topics: Alanine; Amino Acid Motifs; Bacterial Proteins; Carbon-Sulfur Lyases; Cloning, Molecular; Cysteine; Disulfides; Dithionitrobenzoic Acid; Escherichia coli; Escherichia coli Proteins; Iron; Models, Chemical; Mutagenesis, Site-Directed; Plasmids; RNA, Transfer; Spectrophotometry; Sulfur; Sulfurtransferases; Thiamine; Thiouridine; Time Factors; Ultraviolet Rays; Uracil | 2001 |
Modifications of cysteine residues in the solution and membrane-associated conformations of phosphatidylinositol transfer protein have differential effects on lipid transfer activity.
Topics: Animals; Biological Transport, Active; Carrier Proteins; Colorimetry; Cysteine; Disulfides; Dithionitrobenzoic Acid; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Membrane Proteins; Mercaptoethanol; Mutagenesis, Site-Directed; Phosphatidylinositols; Phospholipid Transfer Proteins; Phospholipids; Protein Conformation; Protein Denaturation; Pyridines; Rats; Solutions; Sulfhydryl Compounds | 2001 |
Redox modulation of T-type calcium channels in rat peripheral nociceptors.
Topics: Analysis of Variance; Animals; Calcium Channels, T-Type; Cell Line; Cells, Cultured; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Female; Ganglia, Spinal; Hot Temperature; Humans; Hyperalgesia; Membrane Potentials; Neurons; Neurons, Afferent; Nociceptors; Oxidation-Reduction; Pain; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Recombinant Proteins; Skin; Transfection | 2001 |
The importance of zinc-binding to the function of Rhodobacter sphaeroides ChrR as an anti-sigma factor.
Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins; Cysteine; Dithionitrobenzoic Acid; DNA-Directed RNA Polymerases; Metalloproteins; Molecular Sequence Data; Protein Binding; Protein Interaction Mapping; Rhodobacter sphaeroides; Sequence Alignment; Sigma Factor; Transcription Factors; Transcription, Genetic; Zinc | 2001 |
On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobin.
Topics: Amides; Animals; Circular Dichroism; Cysteine; Disulfides; Dithionitrobenzoic Acid; Guanidine; Hydrogen; Kinetics; Methyl Methanesulfonate; Models, Molecular; Mutation; Myoglobin; Protein Conformation; Protein Folding; Sulfhydryl Compounds; Thermodynamics; Whales | 2001 |
Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1.
Topics: Animals; Binding Sites; Calcium; Calcium-Binding Proteins; Cattle; Cysteine; Disulfides; Dithionitrobenzoic Acid; EF Hand Motifs; Egtazic Acid; Enzyme Activation; Guanylate Cyclase; Guanylate Cyclase-Activating Proteins; Mutation; Nitrobenzoates; Rod Cell Outer Segment; Sulfhydryl Compounds | 2001 |
Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents.
Topics: Binding Sites; Cysteine; Dithionitrobenzoic Acid; Enzyme Inhibitors; Escherichia coli; Glutamate Decarboxylase; Kinetics; Mercuric Chloride; p-Chloromercuribenzoic Acid; Sulfhydryl Reagents | 2001 |
Unfolding rates of barstar determined in native and low denaturant conditions indicate the presence of intermediates.
Topics: Bacterial Proteins; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Enzyme Inhibitors; Guanidine; Kinetics; Protein Denaturation; Protein Folding; Solutions; Spectrometry, Fluorescence; Sulfhydryl Reagents; Urea | 2002 |
Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liver.
Topics: Amino Acid Sequence; Animals; Binding Sites; Catalysis; Chromatography, Gel; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Liver; Molecular Sequence Data; Oxo-Acid-Lyases; Peptide Fragments; Rats; Sequence Homology, Amino Acid; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity; Sulfhydryl Reagents; Trypsin | 2002 |
Recombinant lysine:N(6)-hydroxylase: effect of cysteine-->alanine replacements on structural integrity and catalytic competence.
Topics: Alanine; Calorimetry, Differential Scanning; Cysteine; Dithionitrobenzoic Acid; Enzyme Stability; Flavin-Adenine Dinucleotide; Hot Temperature; Iopanoic Acid; Kinetics; Mixed Function Oxygenases; Models, Chemical; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Conformation; Recombinant Proteins | 2002 |
Conformational change of the E-F interhelical loop in the M photointermediate of bacteriorhodopsin.
Topics: Bacteriorhodopsins; Cysteine; Darkness; Disulfides; Dithionitrobenzoic Acid; Halobacterium salinarum; Kinetics; Light; Models, Molecular; Mutation; Protein Conformation; Protein Engineering; Pyridines; Rotation | 2002 |
Role of cysteine 306 in the catalytic mechanism of Ascaris suum phosphoenolpyruvate carboxykinase.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Ascaris suum; Base Sequence; Catalytic Domain; Cysteine; Deoxyguanine Nucleotides; Dithionitrobenzoic Acid; Enzyme Inhibitors; Escherichia coli; In Vitro Techniques; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphoenolpyruvate Carboxykinase (GTP); Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Homology, Amino Acid; Substrate Specificity; Thermodynamics | 2002 |
The roles of thiols in the bacterial organomercurial lyase (MerB).
Topics: Amino Acid Sequence; Bacterial Proteins; Cysteine; Cytosol; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Hydroxymercuribenzoates; Iodoacetamide; Kinetics; Lyases; Molecular Sequence Data; Mutagenesis, Site-Directed; Organomercury Compounds; Sequence Homology, Amino Acid; Substrate Specificity; Sulfhydryl Compounds | 2002 |
Heterogeneity in the structural dynamics of Sulfolobus solfataricus beta-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry.
Topics: Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Electron Spin Resonance Spectroscopy; Fluorometry; Glucosidases; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Quaternary; Spin Labels; Sulfhydryl Reagents; Sulfolobus; Temperature | 2002 |
Calcium- and myristoyl-dependent properties of guanylate cyclase-activating protein-1 and protein-2.
Topics: Animals; Calcium; Calcium-Binding Proteins; Cattle; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Eye Proteins; Guanylate Cyclase; Guanylate Cyclase-Activating Proteins; Lipid Bilayers; Myristic Acid; Photoreceptor Cells, Vertebrate; Rod Cell Outer Segment; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Surface Plasmon Resonance | 2002 |
Perturbation of microtubule polymerization by quercetin through tubulin binding: a novel mechanism of its antiproliferative activity.
Topics: Anilino Naphthalenesulfonates; Animals; Binding, Competitive; Circular Dichroism; Colchicine; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Goats; Growth Inhibitors; GTP Phosphohydrolases; Kinetics; Microtubules; Polymers; Protein Binding; Quercetin; Solubility; Spectrometry, Fluorescence; Sulfhydryl Reagents; Tubulin; Tubulin Modulators; Vinblastine | 2002 |
Construction of alpha-alpha domains structure in recombinant monkey metallothionein-1.
Topics: Animals; Cadmium; Cysteine; Dithionitrobenzoic Acid; Haplorhini; Hydrogen-Ion Concentration; Metallothionein; Mutation; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Recombinant Proteins | 2002 |
Quantitation and location of disulfide bonds in proteins.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithionitrobenzoic Acid; Electrophoresis; Enzyme-Linked Immunosorbent Assay; Mass Spectrometry; Proteins; Sulfhydryl Compounds | 2003 |
Reaction of 1-amino-2-methylenecyclopropane-1-carboxylate with 1-aminocyclopropane-1-carboxylate deaminase: analysis and mechanistic implications.
Topics: Alanine; Amino Acid Sequence; Carbon-Carbon Lyases; Carboxylic Acids; Catalysis; Chromatography, High Pressure Liquid; Cloning, Molecular; Crotonates; Cyclopropanes; Cysteine; Dithionitrobenzoic Acid; Glycine; Kinetics; Mass Spectrometry; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Pseudomonas; Recombinant Proteins; Serine; Titrimetry | 2003 |
A bicistronic expression system for bacterial production of authentic human interleukin-18.
Topics: Amino Acid Sequence; Base Sequence; Biological Assay; Caspase 1; Caspases; Caspases, Initiator; Codon; Cysteine; Dithionitrobenzoic Acid; DNA, Complementary; Dose-Response Relationship, Drug; Enzyme Activation; Escherichia coli; Gene Library; Humans; Interleukin-18; Methionine; Molecular Sequence Data; Plasmids; Protein Biosynthesis; Protein Structure, Tertiary; Recombinant Proteins; Sulfhydryl Reagents; Temperature; Time Factors; Transcription, Genetic; Ubiquitin | 2003 |
Single-molecule covalent chemistry with spatially separated reactants.
Topics: Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Hemolysin Proteins; Hydrogen-Ion Concentration; Kinetics; Membranes, Artificial; Molecular Conformation; Sulfhydryl Reagents | 2003 |
Antiviral activity of RhoA-derived peptides against respiratory syncytial virus is dependent on formation of peptide dimers.
Topics: Algorithms; Antigens, Viral; Antiviral Agents; Cell Survival; Chromatography, Gel; Cysteine; Dithionitrobenzoic Acid; Humans; Oxidation-Reduction; Peptides; Respiratory Syncytial Virus, Human; rhoA GTP-Binding Protein; Structure-Activity Relationship; Sulfhydryl Reagents; Ultracentrifugation; Viral Plaque Assay | 2003 |
Formation of disulfide bond in p53 correlates with inhibition of DNA binding and tetramerization.
Topics: Binding Sites; Blotting, Western; Chromatography, Gel; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; DNA; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; GADD45 Proteins; Humans; Intracellular Signaling Peptides and Proteins; Maleimides; Microscopy, Atomic Force; Models, Molecular; Molecular Weight; Nitrobenzoates; Nuclear Proteins; Oligonucleotides; Oxidation-Reduction; Polyethylene Glycols; Promoter Regions, Genetic; Protein Binding; Protein Structure, Quaternary; Proteins; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-mdm2; Recombinant Proteins; Sulfenic Acids; Sulfhydryl Compounds; Sulfinic Acids; Sulfonic Acids; Tumor Suppressor Protein p53 | 2003 |
Actin S-glutathionylation: evidence against a thiol-disulphide exchange mechanism.
Topics: Actins; Animals; Chromatography, High Pressure Liquid; Cysteine; Diamide; Dithionitrobenzoic Acid; Glutathione; Glutathione Disulfide; Muscle, Skeletal; Rabbits; Sulfhydryl Compounds | 2003 |
Contribution of cysteines to clathrin trimerization domain stability and mapping of light chain binding.
Topics: Amino Acid Sequence; Animals; Blotting, Western; Cattle; Clathrin; Clathrin Light Chains; Cysteine; Dimerization; Dithionitrobenzoic Acid; Humans; Hydrogen-Ion Concentration; Light; Molecular Sequence Data; Mutation; Protein Binding; Protein Structure, Tertiary; Scattering, Radiation | 2003 |
SpaC and NisC, the cyclases involved in subtilin and nisin biosynthesis, are zinc proteins.
Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins; Bacteriocins; Cysteine; Dithionitrobenzoic Acid; Fourier Analysis; Membrane Proteins; Metalloproteins; Molecular Sequence Data; Nisin; Peptides; Recombinant Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Spectrum Analysis; Zinc | 2003 |
Increased rigidity of domain structures enhances the stability of a mutant enzyme created by directed evolution.
Topics: Alanine; Amino Acid Substitution; Amino Acids; Bacterial Proteins; Cysteine; Directed Molecular Evolution; Dithionitrobenzoic Acid; Enzyme Stability; Hot Temperature; Models, Chemical; Mutagenesis, Site-Directed; Nucleotidyltransferases; Protein Structure, Tertiary; Sulfhydryl Reagents; Thermodynamics | 2003 |
Identification and characterization of a critical region in the glycogen synthase from Escherichia coli.
Topics: Adenosine Diphosphate Glucose; Adenosine Monophosphate; Amino Acid Sequence; Binding Sites; Computer Simulation; Conserved Sequence; Crystallization; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Gene Expression; Glucosephosphates; Glycogen Synthase; Iodoacetic Acid; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Structure-Activity Relationship; Substrate Specificity | 2004 |
Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver.
Topics: Amino Acid Sequence; Animals; Benzophenones; Cattle; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Enzyme Inhibitors; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Inhibitory Concentration 50; Intramolecular Transferases; Kinetics; Mass Spectrometry; Microsomes, Liver; Molecular Sequence Data; Peptide Fragments; Sequence Analysis, Protein; Sequence Homology, Amino Acid | 2004 |
Reversible labeling of cysteine-containing peptides allows their specific chromatographic isolation for non-gel proteome studies.
Topics: Blood Platelets; Blood Proteins; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Humans; Mass Spectrometry; Peptides; Proteome; Sequence Analysis, Protein; Trypsin | 2004 |
Implications of the role of reactive cystein in arginine kinase: reactivation kinetics of 5,5'-dithiobis-(2-nitrobenzoic acid)-modified arginine kinase reactivated by dithiothreitol.
Topics: Arginine Kinase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Reactivators; Kinetics; Magnesium; Substrate Specificity | 2004 |
3alpha/beta,20beta-hydroxysteroid dehydrogenase (porcine testicular carbonyl reductase) also has a cysteine residue that is involved in binding of cofactor NADPH.
Topics: 20-Hydroxysteroid Dehydrogenases; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Hydroxymercuribenzoates; Kinetics; Models, Molecular; NADP; Point Mutation; Protein Binding; Swine | 2004 |
Redox modulation of peripheral T-type Ca2+ channels in vivo: alteration of nerve injury-induced thermal hyperalgesia.
Topics: Animals; Axons; Calcium Channel Blockers; Calcium Channels, T-Type; Cysteine; Disease Models, Animal; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Drug Interactions; Female; Hyperalgesia; Neuralgia; Neurons, Afferent; Nociceptors; Oxidants; Oxidation-Reduction; Pain Measurement; Pain Threshold; Peripheral Nerves; Peripheral Nervous System Diseases; Rats; Rats, Sprague-Dawley; Sciatic Neuropathy | 2004 |
Roles for cysteine residues in the regulatory CXXC motif of human mitochondrial branched chain aminotransferase enzyme.
Topics: Amino Acid Motifs; Catalysis; Cysteine; Dithionitrobenzoic Acid; Humans; Hydrogen Peroxide; Kinetics; Mitochondria; Models, Molecular; Mutation; Oxidation-Reduction; Protein Conformation; Spectrophotometry; Titrimetry; Transaminases | 2004 |
Cysteine-321 of human brain GABA transaminase is involved in intersubunit cross-linking.
Topics: 4-Aminobutyrate Transaminase; Brain; Catalysis; Cross-Linking Reagents; Cysteine; Disulfides; Dithionitrobenzoic Acid; Humans; Mutagenesis, Site-Directed; Protein Structure, Quaternary; Protein Subunits | 2004 |
[Determination of L-cysteine in enzymatic reaction mixture with pre-column derivatization by high performance liquid chromatography].
Topics: Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Mercaptoethanol; Photoelectron Spectroscopy | 2004 |
Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerization.
Topics: 2,2'-Dipyridyl; Adenosine Triphosphate; Amino Acid Sequence; Biopolymers; Cysteine; Disulfides; Dithionitrobenzoic Acid; Guanosine Diphosphate; Guanosine Triphosphate; Kinetics; Methyl Methanesulfonate; Molecular Structure; Nitrobenzoates; Protein Denaturation; Sulfhydryl Compounds; Tubulin | 2005 |
Thermal destabilization of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans upon phosphate binding in the active site.
Topics: Binding Sites; Calorimetry, Differential Scanning; Catalysis; Chlorides; Chromatography, Gel; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); Hot Temperature; Hydrolysis; Kinetics; Ligands; Mutagenesis, Site-Directed; Mutation; Phosphates; Phosphorylation; Protein Binding; Protein Conformation; Protein Denaturation; Streptococcus mutans; Sulfates; Temperature; Time Factors; Ultracentrifugation | 2005 |
Differential effects of cysteine and methionine residues in the antioxidant activity of human serum albumin.
Topics: Antioxidants; Chloramines; Copper; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Ethylmaleimide; Free Radical Scavengers; Free Radicals; Hemolysis; Humans; Lipoproteins, LDL; Methionine; Oxidation-Reduction; Oxidative Stress; Oxygen; Phenanthrolines; Protein Binding; Serum Albumin; Time Factors; Tosyl Compounds | 2005 |
NAD(H) enhances the Cu(II)-mediated inactivation of lactate dehydrogenase by increasing the accessibility of sulfhydryl groups.
Topics: Animals; Binding Sites; Catalysis; Cattle; Copper; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Hydrogen Peroxide; Iron; L-Lactate Dehydrogenase; Lactates; Models, Chemical; NAD; Nucleotides; Oxidation-Reduction; Oxygen; Protein Binding; Protein Conformation; Pyridines; Reactive Oxygen Species; Sulfur; Swine; Time Factors | 2005 |
Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: an example of application for protein chemistry.
Topics: Amino Acid Sequence; Animals; Blotting, Western; Cadaverine; Chickens; Cysteine; Dansyl Compounds; Dithionitrobenzoic Acid; Glutathione; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Molecular Sequence Data; Muscles; Peptide Fragments; Proteins; Sensitivity and Specificity; Sulfhydryl Compounds; Sulfhydryl Reagents | 2005 |
Unfolding mechanism of a hyperthermophilic protein O(6)-methylguanine-DNA methyltransferase.
Topics: Cysteine; Dithionitrobenzoic Acid; Kinetics; Models, Molecular; O(6)-Methylguanine-DNA Methyltransferase; Protein Folding; Temperature; Thermococcus; Thermodynamics | 2005 |
The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid).
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Arginine Kinase; Binding Sites; Catalysis; Cysteine; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; Kinetics; Magnesium; Models, Chemical; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Sea Cucumbers; Spectrometry, Fluorescence; Substrate Specificity; Sulfhydryl Compounds; Time Factors; Tryptophan | 2005 |
Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the hemoglobins of the domestic cat: acetylation of NH3+ terminal group of the beta chain transforms the complex pH dependence of the forward apparent second order rate constant to a simple form.
Topics: Acetylation; Ammonia; Animals; Cats; Chemical Phenomena; Chemistry, Physical; Cysteine; Dithionitrobenzoic Acid; Hemoglobins; Hydrogen-Ion Concentration; Kinetics; Sulfhydryl Compounds; Time Factors | 2006 |
Disulfiram irreversibly aggregates betaine aldehyde dehydrogenase--a potential target for antimicrobial agents against Pseudomonas aeruginosa.
Topics: Alanine; Anti-Infective Agents; Betaine-Aldehyde Dehydrogenase; Binding Sites; Catalysis; Chromatography; Cysteine; Disulfiram; Dithionitrobenzoic Acid; Enzyme Inhibitors; Glutathione; Kinetics; Methyl Methanesulfonate; Models, Chemical; Mutagenesis, Site-Directed; Mutation; NADP; Protein Conformation; Protein Structure, Quaternary; Proteins; Pseudomonas aeruginosa; Sulfhydryl Compounds; Temperature; Time Factors | 2006 |
Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistry.
Topics: Animals; Antibodies; Antibody Specificity; Cysteine; Dithionitrobenzoic Acid; Enzyme-Linked Immunosorbent Assay; Glutathione; Immunohistochemistry; Indicators and Reagents; Iodoacetic Acid; Ligands; Mice; Oxidation-Reduction; Sensitivity and Specificity; Sulfhydryl Compounds; Tissue Fixation | 2006 |
Cloning, expression, purification, and characterization of Vibrio cholerae transcriptional activator, HlyU.
Topics: Bacterial Proteins; Chromatography, Gel; Circular Dichroism; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Guanidine; Models, Genetic; Protein Folding; Solvents; Spectrometry, Fluorescence; Trans-Activators; Transcription Factors; Transcription, Genetic; Vibrio cholerae | 2006 |
Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme.
Topics: Amphibian Proteins; Animals; Catalytic Domain; Chlorocebus aethiops; Cholinesterase Inhibitors; Cholinesterases; COS Cells; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Ethylmaleimide; Inhibitory Concentration 50; Models, Molecular; Mutagenesis, Site-Directed; Sequence Alignment; Sulfhydryl Reagents; Time Factors; Transfection | 2006 |
Crystal structure of the apo-PerR-Zn protein from Bacillus subtilis.
Topics: Bacillus subtilis; Bacterial Proteins; Binding Sites; Crystallization; Crystallography, X-Ray; Cysteine; Dimerization; Dithionitrobenzoic Acid; Gene Expression Regulation, Bacterial; Models, Molecular; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Repressor Proteins; Transcription Factors; Zinc | 2006 |
A thiol labelling competition experiment as a probe for sidechain packing in the kinetic folding intermediate of N-PGK.
Topics: Cysteine; Deuterium Exchange Measurement; Dithionitrobenzoic Acid; Hydrophobic and Hydrophilic Interactions; Kinetics; Molecular Probes; Mutation; Phosphoglycerate Kinase; Protein Denaturation; Protein Folding; Protein Renaturation; Sulfhydryl Compounds | 2006 |
Role of cysteine residues in 4-oxalomesaconate hydratase from Pseudomonas ochraceae NGJ1.
Topics: Amino Acid Sequence; Bacterial Proteins; Circular Dichroism; Cloning, Molecular; Cold Temperature; Crystallography, X-Ray; Cysteine; Dithionitrobenzoic Acid; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Hydro-Lyases; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Pseudomonas; Rhodopseudomonas; Sulfhydryl Reagents; Zinc | 2007 |
Calcium-dependent conformational changes in guanylate cyclase-activating protein 2 monitored by cysteine accessibility.
Topics: Amino Acid Sequence; Animals; Calcium; Cattle; Cysteine; Dithionitrobenzoic Acid; Guanylate Cyclase; Guanylate Cyclase-Activating Proteins; Models, Molecular; Point Mutation; Protein Conformation; Receptors, Cell Surface | 2007 |
Structural and biochemical studies reveal differences in the catalytic mechanisms of mammalian and Drosophila melanogaster thioredoxin reductases.
Topics: Amino Acid Sequence; Animals; Catalysis; Crystallization; Crystallography, X-Ray; Cysteine; Dithionitrobenzoic Acid; Drosophila melanogaster; Mice; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Oligopeptides; Selenocysteine; Thioredoxin-Disulfide Reductase | 2007 |
Transition of hemoglobin between two tertiary conformations: determination of equilibrium and thermodynamic parameters from the reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9[93]beta sulfhydryl group.
Topics: Animals; Binding Sites; Carboxyhemoglobin; Cattle; Cysteine; Dithionitrobenzoic Acid; Hemoglobins; Humans; In Vitro Techniques; Methemoglobin; Oxyhemoglobins; Protein Conformation; Rabbits; Sulfhydryl Reagents; Thermodynamics | 2007 |
Cysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase system.
Topics: Cysteine; Disulfides; Dithionitrobenzoic Acid; Models, Molecular; Mutagenesis, Site-Directed; Peroxidases; Peroxiredoxins; Protein Conformation; Salmonella typhimurium; Sulfhydryl Compounds | 2007 |
Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kinase.
Topics: 2,2'-Dipyridyl; Adenosine Triphosphate; Animals; Creatine; Creatine Kinase; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Kinetics; Molecular Structure; Muscles; Nicotinic Acids; Rabbits; Substrate Specificity; Sulfhydryl Compounds | 2007 |
Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathway.
Topics: Anilino Naphthalenesulfonates; Animals; Binding Sites; Chromatography, Gel; Circular Dichroism; Creatine Kinase; Cysteine; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; Fluorescent Dyes; Guanidine; Kinetics; Models, Biological; Muscles; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Rabbits; Spectrophotometry, Ultraviolet | 2007 |
Biochemical characterization of a novel beta-1--3, 1--4 glucan 4-glucanohydrolase from Thermomonospora sp. having a single active site for lichenan and xylan.
Topics: Actinomycetales; Affinity Labels; Binding Sites; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Glucan 1,4-beta-Glucosidase; Glucans; Hydrolysis; Kinetics; Lysine; o-Phthalaldehyde; Protein Binding; Substrate Specificity; Trinitrobenzenesulfonic Acid; Xylan Endo-1,3-beta-Xylosidase; Xylans | 2007 |
Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site.
Topics: Animals; Arginine; Arginine Kinase; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Inhibitors; Grasshoppers; Insect Proteins; Kinetics; Spectrophotometry; Thermodynamics | 2007 |
Transition of hemoglobin between two tertiary conformations: The transition constant differs significantly for the major and minor hemoglobins of the Japanese quail (Cortunix cortunix japonica).
Topics: Animals; Avian Proteins; Coturnix; Cysteine; Dithionitrobenzoic Acid; Hemoglobins; Hydrogen-Ion Concentration; Protein Structure, Tertiary | 2008 |
Species-specific inhibition of Giardia lamblia triosephosphate isomerase by localized perturbation of the homodimer.
Topics: Animals; Cysteine; Dimerization; Dithionitrobenzoic Acid; Enzyme Stability; Giardia lamblia; Hot Temperature; Methyl Methanesulfonate; Models, Molecular; Protein Structure, Secondary; Protozoan Proteins; Triose-Phosphate Isomerase | 2008 |
Malondialdehyde, a lipid-derived aldehyde alters the reactivity of Cys34 and the esterase activity of serum albumin.
Topics: alpha-Linolenic Acid; Animals; Antioxidants; Cattle; Cysteamine; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Esterases; Fatty Acids; Lipid Peroxidation; Malondialdehyde; Oxidative Stress; Peroxides; Protein Carbonylation; Serum Albumin, Bovine | 2008 |
Compact dimension of denatured states of staphylococcal nuclease.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Mutant Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Salmon; Tryptophan | 2008 |
5,5'-Dithio-bis(2-nitrobenzoic acid) modification of cysteine improves the crystal quality of human chloride intracellular channel protein 2.
Topics: Amino Acid Sequence; Chloride Channels; Crystallization; Crystallography, X-Ray; Cysteine; Dithionitrobenzoic Acid; Humans; Oxidation-Reduction | 2008 |
Effect of cysteine modification on creatine kinase aggregation.
Topics: Animals; Biocatalysis; Catalytic Domain; Creatine Kinase; Cysteine; DDT; Dithionitrobenzoic Acid; Enzyme Stability; Glycine; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Kinetics; Proline; Protein Binding; Protein Multimerization; Protein Structure, Quaternary; Rabbits; Thermodynamics; Zinc | 2009 |
Transition of haemoglobin between two tertiary conformations: Inositol hexakisphosphate increases the transition constant and the affinity of sheep haemoglobin for 5,5'-dithiobis(2-nitrobenzoate).
Topics: Amino Acid Sequence; Animals; Carboxyhemoglobin; Cysteine; Dithionitrobenzoic Acid; Hemoglobins; Hydrogen-Ion Concentration; Kinetics; Phytic Acid; Protein Conformation; Protein Structure, Tertiary; Sheep | 2009 |
Nucleotide dependent cysteine reactivity of hGBP1 uncovers a domain movement during GTP hydrolysis.
Topics: Amino Acid Substitution; Binding Sites; Cysteine; Dithionitrobenzoic Acid; GTP-Binding Proteins; Guanosine Triphosphate; Humans; In Vitro Techniques; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Recombinant Proteins; Sulfhydryl Reagents | 2009 |
Soybean (Glycine max) urease: significance of sulfhydryl groups in urea catalysis.
Topics: Biocatalysis; Cysteine; Dithionitrobenzoic Acid; Enzyme Assays; Ethylmaleimide; Glycine max; Iodoacetamide; Kinetics; p-Chloromercuribenzoic Acid; Plant Proteins; Sulfhydryl Compounds; Sulfhydryl Reagents; Time Factors; Urea; Urease | 2010 |
Cysteines in the loop between IS5 and the pore helix of Ca(V)3.1 are essential for channel gating.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Calcium Channels, T-Type; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; HEK293 Cells; Humans; Ion Channel Gating; Mice; Models, Molecular; Molecular Sequence Data; Patch-Clamp Techniques; Protein Structure, Tertiary | 2010 |
Site-directed mutagenesis of mouse glutathione transferase P1-1 unlocks masked cooperativity, introduces a novel mechanism for 'ping pong' kinetic behaviour, and provides further structural evidence for participation of a water molecule in proton abstract
Topics: Amino Acid Substitution; Animals; Biocatalysis; Catalytic Domain; Crystallography, X-Ray; Cysteine; Dinitrochlorobenzene; Dithionitrobenzoic Acid; Glutathione; Glutathione S-Transferase pi; Hydrogen-Ion Concentration; Kinetics; Mice; Models, Chemical; Models, Molecular; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Protons; Recombinant Proteins; Water | 2011 |
Tropomyosin is in a reduced state in rabbit psoas muscle.
Topics: Animals; Cysteine; Disulfides; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Heart Failure; Humans; Octoxynol; Oxidation-Reduction; Psoas Muscles; Rabbits; Tropomyosin | 2011 |
Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments.
Topics: Amino Acid Sequence; Cysteine; Disulfides; Dithionitrobenzoic Acid; Ions; Molecular Sequence Data; Nitrobenzoates; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds | 2011 |
Hydrogen peroxide targets the cysteine at the active site and irreversibly inactivates creatine kinase.
Topics: Acetylcysteine; Anilino Naphthalenesulfonates; Binding Sites; Brain; Catalytic Domain; Creatine Kinase, BB Form; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; Humans; Hydrogen Peroxide; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Protein Binding; Reactive Oxygen Species; Spectrometry, Fluorescence; Structure-Activity Relationship; Sulfhydryl Compounds | 2011 |
Atypical thioredoxins in poplar: the glutathione-dependent thioredoxin-like 2.1 supports the activity of target enzymes possessing a single redox active cysteine.
Topics: Amino Acid Sequence; Base Sequence; Catalytic Domain; Chloroplast Proteins; Cysteine; Cytosol; Dithionitrobenzoic Acid; Enzyme Activation; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Glutaredoxins; Glutathione; Iron-Sulfur Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP; Oxidation-Reduction; Oxidoreductases; Plant Cells; Plant Proteins; Plastids; Populus; Protein Isoforms; Recombinant Fusion Proteins; Solubility; Spectrometry, Mass, Electrospray Ionization; Substrate Specificity; Thioredoxin-Disulfide Reductase; Thioredoxins | 2012 |
Catalytic zinc site and mechanism of the metalloenzyme PR-AMP cyclohydrolase.
Topics: Amino Acid Sequence; Catalytic Domain; Cysteine; Dithionitrobenzoic Acid; Hydrolases; Magnesium; Metalloproteins; Methanococcus; Models, Molecular; Molecular Sequence Data; Point Mutation; Sequence Alignment; Zinc | 2012 |
Supported oligomethionine sulfoxide and Ellman's reagent for cysteine bridges formation.
Topics: Cysteine; Dimethyl Sulfoxide; Disulfides; Dithionitrobenzoic Acid; Methionine; Molecular Structure; Oxidants; Oxidation-Reduction; Safrole | 2013 |
Evaluation of protein disulfide conversion in vitro using a continuous flow dialysis system.
Topics: Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteamine; Cysteine; Dialysis; Disulfides; Dithionitrobenzoic Acid; Immunoglobulin Fc Fragments; Protein Refolding; Recombinant Fusion Proteins; Tandem Mass Spectrometry | 2013 |
Determination of biothiols by a novel on-line HPLC-DTNB assay with post-column detection.
Topics: Chromatography, High Pressure Liquid; Cysteine; Dithioerythritol; Dithionitrobenzoic Acid; Glutathione; Oligosaccharides; Pharmaceutical Preparations; Sulfhydryl Compounds | 2012 |
A conformational intermediate in glutamate receptor activation.
Topics: Benzothiadiazines; Biophysical Phenomena; Cell Line, Transformed; Crystallography, X-Ray; Cysteine; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Electric Stimulation; Excitatory Amino Acid Antagonists; Glutamic Acid; Humans; In Vitro Techniques; Membrane Potentials; Models, Molecular; Molecular Conformation; Mutation; Patch-Clamp Techniques; Phenanthrolines; Protein Structure, Tertiary; Quinoxalines; Receptors, Glutamate; Uncoupling Agents | 2013 |
A chromogenic assay of substrate depletion by thiol dioxygenases.
Topics: Animals; Chromogenic Compounds; Cysteine; Cysteine Dioxygenase; Dithionitrobenzoic Acid; Enzyme Activators; Enzyme Assays; Enzyme Inhibitors; Rats; Substrate Specificity | 2014 |
Mia40 is optimized for function in mitochondrial oxidative protein folding and import.
Topics: Catalytic Domain; Cation Transport Proteins; Copper Transport Proteins; Cysteine; Disulfides; Dithionitrobenzoic Acid; Escherichia coli Proteins; Mitochondrial Membrane Transport Proteins; Mitochondrial Precursor Protein Import Complex Proteins; Models, Molecular; Molecular Chaperones; Protein Conformation; Protein Disulfide-Isomerases; Protein Folding; Protein Stability; Protein Transport; Saccharomyces cerevisiae Proteins | 2014 |
Intermolecular disulfide bond formation promotes immunoglobulin aggregation: investigation by fluorescence correlation spectroscopy.
Topics: Animals; Cattle; Cysteine; Disulfides; Dithionitrobenzoic Acid; Guanidine; Immunoglobulin G; Kinetics; Organophosphorus Compounds; Protein Aggregates; Protein Denaturation; Spectrometry, Fluorescence | 2015 |
Quantification of PEG-maleimide ligands and coupling efficiencies on nanoparticles with Ellman's reagent.
Topics: Coloring Agents; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Ligands; Maleimides; Nanoparticles; Polyethylene Glycols; Polystyrenes | 2015 |
A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II).
Topics: Copper; Cysteine; Dithionitrobenzoic Acid; Fluorescent Dyes; HeLa Cells; Humans; Spectrometry, Fluorescence; Sulfhydryl Compounds | 2016 |
Evidence for redox sensing by a human cardiac calcium channel.
Topics: Calcium Channels, L-Type; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Humans; Mutation; Myocardium; Oxidants; Oxidation-Reduction; Protein Folding; Protein Isoforms; Sulfhydryl Compounds | 2016 |
Probing bulky ligand entry in engineered archaeal ferritins.
Topics: Archaea; Archaeoglobus fulgidus; Cysteine; Dithionitrobenzoic Acid; Ferritins; Kinetics; Ligands; Mutation; Pyrococcus furiosus; Sulfhydryl Compounds | 2017 |
Determination of thiol-to-protein ratio and drug-to-antibody ratio by in-line size exclusion chromatography with post-column reaction.
Topics: Aminobenzoates; Antibodies, Monoclonal; Calibration; Chromatography, Gel; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Humans; Immunoconjugates; Immunoglobulin G; Kinetics; Oligopeptides; Oxidation-Reduction; Reproducibility of Results; Sensitivity and Specificity; Sulfhydryl Compounds | 2017 |
Functional comparision between truncated MTT1 and truncated MTT2 from Tetrahyemna thermophila.
Topics: Amino Acid Sequence; Cadmium; Copper; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Metallothionein; Protein Stability; Protozoan Proteins; Sequence Deletion; Stress, Physiological; Tetrahymena | 2018 |
Cysteine Disulfide Traps Reveal Distinct Conformational Ensembles in Dengue Virus NS2B-NS3 Protease.
Topics: Cysteine; Dengue Virus; Disulfides; Dithionitrobenzoic Acid; Escherichia coli; Pliability; Protein Conformation; Serine Endopeptidases; Serine Proteinase Inhibitors; Sulfones; Viral Nonstructural Proteins | 2019 |
Simple and Novel Assay of the Host-Guest Complexation of Homocysteine with Cucurbit[7]uril.
Topics: Antibodies, Monoclonal; Biological Assay; Bridged-Ring Compounds; Cystathionine gamma-Lyase; Cysteine; Dithionitrobenzoic Acid; Epitopes; Homocysteine; Humans; Imidazoles; Models, Molecular; Molecular Structure; Sulfhydryl Reagents | 2019 |
Ultra-Rapid Glutathionylation of Ribonuclease: Is this the Real Incipit of its Oxidative Folding?
Topics: Animals; Cattle; Cysteine; Disulfides; Dithionitrobenzoic Acid; Glutathione; Glutathione Disulfide; Hydrogen-Ion Concentration; Oxidation-Reduction; Oxidative Stress; Protein Disulfide-Isomerases; Protein Folding; Ribonuclease, Pancreatic; Ribonucleases; Sulfhydryl Compounds; Tandem Mass Spectrometry | 2019 |
Targeting an Intrinsically Disordered Protein by Covalent Modification.
Topics: Calcium-Binding Proteins; Calpain; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Drug Design; Electrophoresis, Polyacrylamide Gel; Humans; Interferometry; Intrinsically Disordered Proteins; Kinetics; Molecular Structure; Molecular Targeted Therapy; Protein Binding; Structure-Activity Relationship; Tandem Mass Spectrometry | 2020 |
Cross-talk between the calcium channel TRPV4 and reactive oxygen species interlocks adhesive and degradative functions of invadosomes.
Topics: Actins; Animals; Calcium; Cell Adhesion; Cysteine; Dithionitrobenzoic Acid; Extracellular Matrix; HEK293 Cells; Humans; Hydrogen Peroxide; Integrin beta1; MAP Kinase Kinase Kinase 5; Mice; Models, Biological; Myosin Type I; Podosomes; Protein Transport; RAW 264.7 Cells; Reactive Oxygen Species; TRPV Cation Channels | 2021 |
Determination of low molecular thiols and protein sulfhydryl groups using heterocyclic disulfides.
Topics: Cysteine; Disulfides; Dithionitrobenzoic Acid; Glutathione; Serum Albumin, Bovine; Sulfhydryl Compounds | 2022 |
Selective Homocysteine Assay with Cucurbit[7]uril by pH Regulation.
Topics: Cysteine; Dithionitrobenzoic Acid; Heterocyclic Compounds, 2-Ring; Homocysteine; Homocystine; Hydrogen-Ion Concentration; Imidazolidines; Macrocyclic Compounds | 2022 |