dinoprost and mesotocin

The oxytocic peptide mesotocin was measured in plasma samples collected throughout pregnancy in the conscious tammar wallaby, Macropus eugenii. Plasma mesotocin and the prostaglandin metabolite 13,14-dihydro-15-oxo-prostaglandin F2 alpha were also assessed immediately prepartum and during parturition. A radioimmunoassay for mesotocin was validated in the tammar and this assay allowed direct measurement in 50 microliters unextracted plasma with a sensitivity of 12.5 pmol l-1. Plasma concentrations of mesotocin remained basal (approximately 15 pmol l-1) at all stages of pregnancy, including prepartum. A significant (P < 0.05) increase in plasma mesotocin was observed only immediately after delivery of the neonate and this increase was maintained for at least 15 min postpartum. Mesotocin concentrations returned to basal values 2 h after birth. Peak concentrations of mesotocin of 516.7 +/- 108.1 pmol l-1 were measured within 2 min of birth. This peak coincided with a short-lived peak in concentration of prostaglandin F2 alpha metabolite immediately after birth (2.1 +/- 0.4 nmol l-1) which decreased to less than 0.3 nmol l-1 within 2 h postpartum. These data demonstrate that mesotocin is released during, or immediately after, delivery and appears to parallel the profile of circulating prostaglandin F2 alpha metabolite in this marsupial.

Topics: Animals; Dinoprost; Female; Labor, Obstetric; Macropodidae; Oxytocin; Postpartum Period; Pregnancy; Pregnancy, Animal; Radioimmunoassay

The oxytocin receptor antagonist [1-deamino-2-D-Tyr-(OEt)-4-Thr-8-Om]-oxytocin (Atosiban) is a specific antagonist of both mesotocin- and oxytocin-induced myometrial contractions in late pregnant tammars in vitro. Continuous intravenous infusion of Atosiban (1 mg kg-1 day-1) for 3 or 7 days from day 24 of the 26.5 day gestation significantly delayed births. In both the 3 day and 7 day infusion groups, all 15 control animals were pregnant and gave birth within the normal time (day 26.75 +/- 0.20, mean +/- SEM), during the infusion of saline. The neonates weighed 387 +/- 8 mg. Deliveries were observed in 15 Atosiban-treated animals significantly (P < 0.05) later than in the controls (day 27.85 +/- 0.19; neonate weight 413 +/- 9 mg). All pouch young were successfully suckled, even in the continued presence of Atosiban. Baseline plasma concentrations of the prostaglandin F metabolite (PGFM) in pregnant tammars were < 200 pg ml-1. A surge in plasma PGFM occurred at birth (811 +/- 116 pg ml-1), followed by a rapid fall to baseline concentrations within 1 h after birth. This was observed both in saline- and in Atosiban-treated animals that gave birth during the observation period, and did not differ significantly between the treatment groups. Plasma progesterone concentrations in the control and the Atosiban-treated animals showed the normal pattern of luteolysis immediately after birth. Thus, infusion of an oxytocin receptor antagonist at the end of gestation delays birth, the peripartum surge in prostaglandin release, and the fall in progesterone, suggesting that mesotocin is an important part of the hormonal cascade associated with delivery in this marsupial.

Topics: Animals; Dinoprost; Female; In Vitro Techniques; Labor, Obstetric; Macropodidae; Myometrium; Oxytocin; Pregnancy; Progesterone; Receptors, Oxytocin; Tocolytic Agents; Vasotocin

Gravid, female American toads are known to move toward conspecific mating calls. This behavior, as well as ovulation, often can be induced by the injection of human chorionic gonadotropin (HCG). It was found that HCG-induced phonotaxis could be terminated by the injection of indomethacin (an inhibitor of prostaglandin synthesis) and then reinstated by the injection of prostaglandin F2 alpha. A drug regimen was devised that allowed elicitation of phonotaxis, indistinguishable from that induced by HCG and in the absence of ovulation. This involved the administration of prostaglandin F2 alpha and arginine vasotocin (or mesotocin) following progesterone priming. It has not been determined whether the peptide is really essential.

Topics: Abdominal Muscles; Acoustic Stimulation; Animals; Bufonidae; Chorionic Gonadotropin; Dinoprost; Estradiol; Female; Indomethacin; Muscle Contraction; Oxytocin; Progesterone; Prostaglandins; Prostaglandins F; Sexual Behavior, Animal; Vasotocin; Vocalization, Animal