dinoprost and dolasetron

Chondrocytes are one of the main cell types involved in rheumatoid inflammation, releasing mediators which add to cartilage destruction, bone damage and consequently disability. Current evidence suggests that serotonin 5-HT(3) receptor antagonists (5-HT(3)RA) show anti-inflammatory and antioxidant properties in vitro and in vivo. Yet, the mechanisms of the anti-inflammatory effects of 5-HT(3)RA have not been elucidated in detail.. Therefore, we examined in detail the effects of 5-HT(3)RA on inflammatory parameters in human primary chondrocytes in vitro by studying prostaglandin E2 (PGE2) and 8-isoprostane (8-iso-PGF2α) levels by EIA and interleukin-6 (IL-6) synthesis by ELISA. Cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) protein levels were analyzed by Western blot.. We found a significant reduction of IL-1β induced PGE2, 8-iso-PGF2β and IL-6 chondrocytes by 5-HT(3)RA especially by dolasetron.. This study provides additional support to the potential use of 5-HT(3)RAs as therapeutic agents to reduce joint inflammation.

Topics: Arthritis, Rheumatoid; Cells, Cultured; Chondrocytes; Cyclooxygenase 2; Dinoprost; Dinoprostone; Humans; Indoles; Interleukin-1beta; Interleukin-6; Intramolecular Oxidoreductases; Primary Cell Culture; Prostaglandin-E Synthases; Quinolizines; Receptors, Serotonin, 5-HT3; Serotonin 5-HT3 Receptor Antagonists