dinoprost and 8-oxo-7-8-dihydrodeoxyguanine

To study oxidative stress differences between women with normal vaginal deliveries (VD) and those with elective cesarean sections without labor (CS), total antioxidant capacity (TAC), erythrocyte glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity, 8-isoprostane, nitrotyrosine and 8-hydroxydeoxyguanosine (8-OHdG) in blood, urine, and placental samples were assessed. The VD group had significantly higher placental 8-isoprostane and 8-OHdG levels as well as greater plasma TAC and 8-OHdG levels and lower erythrocyte SOD activity in umbilical venous blood. Women with VD exhibited differential changes in maternal oxidative stress before and after delivery compared with women with CS. Furthermore, we found that repetitive hypoxia-reoxygenation increased the 8-isoprostane and 8-OHdG levels in villous explants compared with the normoxic controls. Together, these results indicate that labor is associated with increased placental oxidative stress and has an influence on maternal oxidative stress. Therefore, women with VD exhibit different oxidative stress indicators than do those with CS.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Antioxidants; Cesarean Section; Delivery, Obstetric; Dinoprost; Erythrocytes; Female; Glutathione Peroxidase; Guanine; Humans; Hypoxia; Labor, Obstetric; Oxidative Stress; Placenta; Pregnancy; Superoxide Dismutase; Umbilical Veins

DNA integrity was investigated in the lymphocytes of 50 bus drivers, 20 garagemen and 50 controls using the comet assay with excision repair enzymes. In parallel, 8-oxo-7,8-dihydro-2'-deoxyguanosine and 15-F(2t)-isoprostane levels in the urine and protein carbonyl levels in the plasma were assessed as markers of oxidative damage to DNA, lipids and proteins. Exposure to carcinogenic polycyclic aromatic hydrocarbons (cPAHs) and volatile compounds was measured by personal samplers for 48 and 24h, respectively, before the collection of biological specimens. Both exposed groups exhibited a higher levels of DNA instability and oxidative damage to biological macromolecules than the controls. The incidence of oxidized lesions in lymphocyte DNA, but not the urinary levels of 8-oxodG, correlated with exposure to benzene and triglycerides increased this damage. Oxidative damage to lipids and proteins was associated with exposure to cPAHs and the lipid peroxidation levels positively correlated with age and LDL cholesterol, and negatively with vitamin C. The carriers of at least one variant hOGG1 (Cys) allele tended to higher oxidative damage to lymphocyte DNA than those with the wild genotype, while XPD23 (Gln/Gln) homozygotes were more susceptible to the induction of DNA strand breaks. In contrast, GSTM1 null variant seemed to protect DNA integrity.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Air Pollutants, Occupational; Air Pollution; Comet Assay; Dinoprost; DNA; DNA Damage; DNA Glycosylases; Genetic Predisposition to Disease; Glutathione Transferase; Guanine; Humans; Lipid Peroxidation; Lymphocytes; Male; Oxidative Stress; Polycyclic Aromatic Hydrocarbons; Polymorphism, Genetic; Protein Carbonylation; Vasoconstrictor Agents; Vehicle Emissions; Volatile Organic Compounds; Xeroderma Pigmentosum Group D Protein