dimethyloxalylglycine and cobaltous-chloride

dimethyloxalylglycine has been researched along with cobaltous-chloride* in 2 studies

Other Studies

2 other study(ies) available for dimethyloxalylglycine and cobaltous-chloride

Article	Year
Hypoxia-inducible factor 1α (HIF-1α) is a major determinant in the enhanced function of muscle-derived progenitors from MRL/MpJ mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019, Volume: 33, Issue:7 Topics: Animals; Cobalt; Gene Expression Regulation; Glycine; Hypoxia-Inducible Factor 1, alpha Subunit; Mice; Mice, Knockout; Muscle, Skeletal; PAX7 Transcription Factor; Procollagen-Proline Dioxygenase; Regeneration; Stem Cells	2019
Light-Emitting Diode Irradiation (640 nm) Regulates Keratinocyte Migration and Cytoskeletal Reorganization Via Hypoxia-Inducible Factor-1α. Photomedicine and laser surgery, 2016, Volume: 34, Issue:8 The objective of this study was to determine the effect of light-emitting diode (LED) irradiation on the migration and proliferation of keratinocytes.. Keratinocytes play a key role in re-epithelialization during wound healing; it is speculated that low-level LED therapy might improve keratinocyte migration and proliferation.. Human keratinocyte cells (HKCs) were isolated from child or adult foreskins and irradiated with LED light with a wavelength of 640 nm and a dosage of 12 or 24 J/cm(2). Cell motility, migration, and proliferation were examined using live cell imaging, scratch assay, and a colorimetric cell counting assay, respectively. Hypoxia-inducible factor-1α (HIF-1α) protein levels were analyzed by using Western blotting. Filamentous actin (F-actin) was stained by phalloidin. YC-1 [3-(5-hydroxymethyl-2-furyl)-1-benzylindazole] was used as an HIF-1 inhibitor, and CoCl2 (cobalt chloride) and DMOG (dimethyloxaloyl glycine) are HIF-1α activators.. LED irradiation significantly promoted cell motility and migration, but did not significantly influence cell proliferation in HKCs. Furthermore, LED irradiation resulted in a reorganization of cellular F-actin and a dramatic upregulation of HIF-1α expression. Suppression of HIF-1α using the compound YC-1 prevented reorganization of the actin cytoskeleton following LED irradiation, suggesting that the effect of LED irradiation on the cytoskeleton is mediated through HIF-1α. Conversely, chemical activation of HIF-1α via DMOG or CoCl2 resulted in a reorganization of F-actin.. LED irradiation may increase keratinocyte migration via HIF-1α-dependent cytoskeletal reorganization. Topics: Adult; Blotting, Western; Cell Movement; Cell Proliferation; Child; Cobalt; Cytoskeleton; Foreskin; Glycine; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Indazoles; Keratinocytes; Low-Level Light Therapy; Male; Up-Regulation	2016

Article

Year

Hypoxia-inducible factor 1α (HIF-1α) is a major determinant in the enhanced function of muscle-derived progenitors from MRL/MpJ mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019, Volume: 33, Issue:7

Topics: Animals; Cobalt; Gene Expression Regulation; Glycine; Hypoxia-Inducible Factor 1, alpha Subunit; Mice; Mice, Knockout; Muscle, Skeletal; PAX7 Transcription Factor; Procollagen-Proline Dioxygenase; Regeneration; Stem Cells

2019

Light-Emitting Diode Irradiation (640 nm) Regulates Keratinocyte Migration and Cytoskeletal Reorganization Via Hypoxia-Inducible Factor-1α.

Photomedicine and laser surgery, 2016, Volume: 34, Issue:8

The objective of this study was to determine the effect of light-emitting diode (LED) irradiation on the migration and proliferation of keratinocytes.. Keratinocytes play a key role in re-epithelialization during wound healing; it is speculated that low-level LED therapy might improve keratinocyte migration and proliferation.. Human keratinocyte cells (HKCs) were isolated from child or adult foreskins and irradiated with LED light with a wavelength of 640 nm and a dosage of 12 or 24 J/cm(2). Cell motility, migration, and proliferation were examined using live cell imaging, scratch assay, and a colorimetric cell counting assay, respectively. Hypoxia-inducible factor-1α (HIF-1α) protein levels were analyzed by using Western blotting. Filamentous actin (F-actin) was stained by phalloidin. YC-1 [3-(5-hydroxymethyl-2-furyl)-1-benzylindazole] was used as an HIF-1 inhibitor, and CoCl2 (cobalt chloride) and DMOG (dimethyloxaloyl glycine) are HIF-1α activators.. LED irradiation significantly promoted cell motility and migration, but did not significantly influence cell proliferation in HKCs. Furthermore, LED irradiation resulted in a reorganization of cellular F-actin and a dramatic upregulation of HIF-1α expression. Suppression of HIF-1α using the compound YC-1 prevented reorganization of the actin cytoskeleton following LED irradiation, suggesting that the effect of LED irradiation on the cytoskeleton is mediated through HIF-1α. Conversely, chemical activation of HIF-1α via DMOG or CoCl2 resulted in a reorganization of F-actin.. LED irradiation may increase keratinocyte migration via HIF-1α-dependent cytoskeletal reorganization.

Topics: Adult; Blotting, Western; Cell Movement; Cell Proliferation; Child; Cobalt; Cytoskeleton; Foreskin; Glycine; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Indazoles; Keratinocytes; Low-Level Light Therapy; Male; Up-Regulation

2016