dimethylarsinoylacetic-acid and arsenobetaine

dimethylarsinoylacetic-acid has been researched along with arsenobetaine* in 3 studies

Other Studies

3 other study(ies) available for dimethylarsinoylacetic-acid and arsenobetaine

ArticleYear
Deuterium exchange in arsenobetaine and dimethylarsinoylacetic acid.
    Journal of inorganic biochemistry, 2005, Volume: 99, Issue:3

    Arsenobetaine occurs naturally in almost all marine animals and it is assumed to be the unreactive end-product of a detoxification pathway. To investigate the properties of arsenobetaine and its likely immediate biogenic precursor, dimethylarsinoylacetic acid, we studied the exchanges of the C-2 methylene protons of these compounds in D2O solution and showed them to be pH dependent first-order reactions. For arsenobetaine, the rate of exchange was highest at high pH values although exchange also occurred at low pH values. For dimethylarsinoylacetic acid, the rate was highest at low pH values although there was also exchange at high pH values. The half-life of the reaction was maximum for arsenobetaine at pH values of 5-6, and for dimethylarsinoylacetic acid at 6.5-8.5. Mechanisms are suggested for the exchange reactions involved.

    Topics: Acetates; Animals; Arsenicals; Buffers; Deuterium; Hydrocarbons; Hydrogen-Ion Concentration; Kinetics; Magnetic Resonance Spectroscopy; Marine Biology; Methane; Models, Chemical; Protons

2005
An origin for arsenobetaine involving bacterial formation of an arsenic-carbon bond.
    FEMS microbiology letters, 2004, Jun-01, Volume: 235, Issue:1

    Lysed-cell extract of a Pseudomonas sp. was shown to catalyse bioconversion of dimethylarsinoylacetate to arsenobetaine and dimethylarsinate. Provision of the universal methyl donor S-adenosylmethionine to bioconversion mixtures promoted both the rate and extent of arsenobetaine formation. These findings suggest that in the proposed biosynthesis of arsenobetaine from dimethylarsinoylethanol, oxidation (i.e. the formation of the carboxymethyl group of dimethylarsinoylacetate) would precede the reduction and methylation at the arsenic atom. The presence of enzyme(s) capable of methylating dimethylarsinoylacetate in a bacterial isolate from marine mussel (Mylitus edulis), highlights a possible direct involvement of prokaryotic organisms in the biosynthesis of organoarsenic compounds within marine animals.

    Topics: Acetates; Arsenic; Arsenicals; Carbon; Methylation; Pseudomonas; S-Adenosylmethionine

2004
Bacterial degradation of arsenobetaine via dimethylarsinoylacetate.
    Archives of microbiology, 2003, Volume: 180, Issue:2

    Microorganisms from Mytilus edulis (marine mussel) degraded arsenobetaine, with the formation of trimethylarsine oxide, dimethylarsinate and methylarsonate. Four bacterial isolates from these mixed-cultures were shown by HPLC/hydride generation-atomic fluorescence spectroscopy (HPLC/HG-AFS) analysis to degrade arsenobetaine to dimethylarsinate in pure culture; there was no evidence of trimethylarsine oxide formation. Two of the isolates ( Paenibacillussp. strain 13943 and Pseudomonas sp. strain 13944) were shown by HPLC/inductively coupled plasma-mass spectrometry (HPLC/ICPMS) analysis to degrade arsenobetaine by initial cleavage of a methyl-arsenic bond to form dimethylarsinoylacetate, with subsequent cleavage of the carboxymethyl-arsenic bond to yield dimethylarsinate. Arsenobetaine biodegradation by pure cultures was biphasic, with dimethylarsinoylacetate accumulating in culture supernatants during the culture growth phase and its removal accompanying dimethylarsinate formation during a carbon-limited stationary phase. The Paenibacillus sp. also converted exogenously supplied dimethylarsinoylacetate to dimethylarsinate only under carbon-limited conditions. Lysed-cell extracts of the Paenibacillus sp. showed constitutive expression of enzyme(s) capable of arsenobetaine degradation through methyl-arsenic and carboxymethyl-arsenic bond cleavage. The work establishes the capability of particular bacteria to cleave both types of arsenic-carbon bonds of arsenobetaine and demonstrates that mixed-community functioning is not an obligate requirement for arsenobetaine biodegradation.

    Topics: Acetates; Aeromonas; Animals; Arsenicals; Bacteria; Biodegradation, Environmental; Bivalvia; Cacodylic Acid; Chromatography, High Pressure Liquid; Mass Spectrometry; Pseudomonas

2003