digitonin and cesium-chloride

The internal pH value (pHi) of the long-slender bloodstream form of Trypanosoma brucei was estimated from the distribution of 14C-labeled 5,5-dimethyl-2,4-oxazolidinedione or 14C-labeled methyl amine between the intracellular space of the cells and the medium. The pHi of T. brucei remained relatively constant at 7.0-7.2 throughout an extracellular pH (pHo) range of 6.0-8.0. The maintenance of an internal pH more acidic than the environment appears to be a unique feature. Preincubation of T. brucei with carbonyl cyanide m-chlorophenyl hydrazone (CCCP) or CCCP + valinomycin had no appreciable effect on the delta pH across the T. brucei membrane when the external pH was 8.0. However, when the external pH was 6.0, CCCP abolished the observed delta pH. Nigericin significantly dissipated the delta pH across the T. brucei membrane at all pHo values. These data suggest that under physiological conditions, the maintenance of a delta pH across the bloodstream-form T. brucei membrane may be by a mechanism other than an energy-dependent gradient, whereas an energy-dependent pump may be needed for maintaining the pHi in an acidic environment. The electrical potential (delta psi) across the trypanosomal plasma membrane was also estimated using the lipophilic cation, [3H]tetraphenyl-phosphonium bromide. It appears dependent on both the external pH and the external salt conditions. Under ionic conditions similar to the host bloodstream, it ranges from -76 to -160 mV over an external pH range of 6.0 to 8.0, with an estimated value of -155.5 +/- 0.7 at the physiological pH.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Adenosine Triphosphatases; Animals; Biological Transport, Active; Carbonyl Cyanide m-Chlorophenyl Hydrazone; Cell Membrane Permeability; Cesium; Chlorides; Digitonin; Hydrogen-Ion Concentration; Ionophores; Potassium Chloride; Sodium Chloride; Trypanosoma brucei brucei; Valinomycin