diethyl pyrocarbonate and lysine

diethyl pyrocarbonate has been researched along with lysine in 38 studies

Research

Studies (38)

TimeframeStudies, this research(%)All Research%
pre-199011 (28.95)18.7374
1990's20 (52.63)18.2507
2000's5 (13.16)29.6817
2010's2 (5.26)24.3611
2020's0 (0.00)2.80

Authors

AuthorsStudies
Little, C1
Borders, CL; George, AL1
Dykes, CW; Kay, J1
Halpert, J1
Dierks, T; Krämer, R; Salentin, A; Stappen, R1
Macek, P; Turk, T1
Deyashiki, Y; Hara, A; Nakayama, T; Sawada, H; Shinoda, M; Tanabe, H1
Cescatti, L; Menestrina, G; Pederzolli, C1
Maruyama, K1
Butor, C; Diaz, S; Higa, HH; Varki, A1
Lyon, M1
Carrea, G; Mazzola, G; Pasta, P1
Neujahr, HY; Sejlitz, T1
Brtko, J; Knopp, J1
Altendorf, K; Herkenhoff, B; Schmid, R; Schneider, E; Steffens, K1
Kosarev, IV; Surovtsev, VI; Zav'yalov, VP1
Adachi, O; Frébort, I; Luhová, L; Matsushita, K; Pec, P; Toyama, H1
Hughes, PJ; Kirk, CJ; Michell, RH1
Everson, B; Jentoft, J; Ratnoff, OD; Ryder, J1
Chang, WI; Matthews, KS; Olson, JS1
Crankshaw, MW; Myers, RP; Nash, WE; Strickler, RC; Thomas, JL1
Bessman, MJ; Brush, GS1
Chang, LH; Tam, MF1
Christendat, D; Turnbull, J1
Carrea, G; D'Arrigo, P; Secundo, F; Servi, S1
Kopecek, J; Kopecková, P; Shen, HR; Spikes, JD1
Peter-Katalinić, J; Rizner, TL; Strupat, K; Zakelj-Mavric, M; Zorko, M1
Dage, JL; Halsall, HB; Sun, H1
Hao, S; Lu, J; Zhang, H; Zhang, J1
Busto, F; De Arriaga, D; Olano, J; Soler, J1
Carvajal, N; Cerpa, J; Herrera, P; López, V; Olate, J; Salas, M; Uribe, E1
Burk, RF; Caprioli, RM; Hill, KE; Hondal, RJ; Ma, S1
George, SP; Rao, MB1
Relyea, HA; Rimkus, S; van der Donk, WA; Wheatley, JL; Woodyer, R1
Faridmoayer, A; Scaman, CH1
Hnízda, A; Kodícek, M; Sanda, M; Santrůcek, J; Strohalm, M1
Arata, T; Inoue, A; Narumi, R; Yamamoto, T1
Arndt, JR; Kondalaji, SG; Legleiter, J; Maurer, MM; Parker, A; Valentine, SJ1

Other Studies

38 other study(ies) available for diethyl pyrocarbonate and lysine

ArticleYear
The histidine residues of phospholipase C from Bacillus cereus.
    The Biochemical journal, 1977, Nov-01, Volume: 167, Issue:2

    Topics: Bacillus cereus; Binding Sites; Chromatography, Affinity; Diethyl Pyrocarbonate; Histidine; Kinetics; Lysine; Phospholipases; Structure-Activity Relationship

1977
Chemical modification of histidyl and lysyl residues in yeast enolase.
    Biochimica et biophysica acta, 1979, Jul-11, Volume: 569, Issue:1

    Topics: Binding Sites; Diethyl Pyrocarbonate; Enzyme Reactivators; Histidine; Hydroxylamines; Lysine; Osmolar Concentration; Phosphopyruvate Hydratase; Pyridoxal Phosphate; Substrate Specificity; Temperature; Time Factors; Trinitrobenzenesulfonic Acid; Yeasts

1979
The effect of modification of lysine and histidine residues on the potential activity of pig pepsinogen [proceedings].
    Biochemical Society transactions, 1977, Volume: 5, Issue:5

    Topics: Animals; Binding Sites; Diethyl Pyrocarbonate; Histidine; Imides; Lysine; Pepsinogens; Swine

1977
Structure and function of neuro- and myotoxic phospholipases: modification with ethoxyformic anhydride of notechis II-5 from the venom of the Australian tiger snake Notechis scutatus scutatus.
    Advances in cytopharmacology, 1979, Volume: 3

    Topics: Animals; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; Egg Yolk; Female; Formates; Histidine; Lysine; Neurotoxins; Phosphatidylcholines; Phospholipases; Snake Venoms; Snakes; Tyrosine

1979
Probing the active site of the reconstituted aspartate/glutamate carrier from bovine heart mitochondria: carbodiimide-catalyzed acylation of a functional lysine residue.
    Biochimica et biophysica acta, 1992, Jan-10, Volume: 1103, Issue:1

    Topics: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; Acylation; Amino Acid Transport Systems, Acidic; Animals; Antiporters; Aspartic Acid; Binding Sites; Biological Transport; Carrier Proteins; Catalysis; Cattle; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; Ethyldimethylaminopropyl Carbodiimide; Glutamates; Glutamic Acid; Kinetics; Lysine; Mitochondria, Heart; Proteolipids; Pyridoxal Phosphate

1992
The role of lysine, histidine and carboxyl residues in biological activity of equinatoxin II, a pore forming polypeptide from the sea anemone Actinia equina L.
    Biochimica et biophysica acta, 1992, Feb-13, Volume: 1119, Issue:1

    Topics: Animals; Cattle; Cnidarian Venoms; Diethyl Pyrocarbonate; Hemolysis; Histidine; Humans; Indicators and Reagents; Isoelectric Focusing; Lipoproteins; Lysine; Molecular Weight; Pyridoxal Phosphate; Sea Anemones; Spectrophotometry

1992
Chemical modification of cysteinyl, lysyl and histidyl residues of mouse liver 17 beta-hydroxysteroid dehydrogenase.
    Biochimica et biophysica acta, 1992, Apr-08, Volume: 1120, Issue:2

    Topics: 17-Hydroxysteroid Dehydrogenases; Animals; Binding Sites; Coenzymes; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Histidine; Ligands; Liver; Lysine; Mice; Protein Binding; Spectrum Analysis; Structure-Activity Relationship; Trinitrobenzenesulfonic Acid

1992
Modification of lysine residues of Staphylococcus aureus alpha-toxin: effects on its channel-forming properties.
    The Journal of membrane biology, 1991, Volume: 119, Issue:1

    Topics: Bacterial Toxins; Diethyl Pyrocarbonate; Electrochemistry; Electrophoresis, Polyacrylamide Gel; Hemolysin Proteins; Histidine; Hydroxylamine; Hydroxylamines; Ion Channels; Isoelectric Focusing; Lipid Bilayers; Lysine; Staphylococcus aureus; Trinitrobenzenesulfonic Acid

1991
Chemical modification of Pseudomonas ochraceae 4-hydroxy-4-methyl-2-oxoglutarate aldolase by diethyl pyrocarbonate.
    Journal of biochemistry, 1991, Volume: 110, Issue:6

    Topics: Amino Acids; Binding Sites; Diethyl Pyrocarbonate; Hydrogen-Ion Concentration; Kinetics; Lysine; Oxo-Acid-Lyases; Pseudomonas

1991
O-acetylation and de-O-acetylation of sialic acids. O-acetylation of sialic acids in the rat liver Golgi apparatus involves an acetyl intermediate and essential histidine and lysine residues--a transmembrane reaction?
    The Journal of biological chemistry, 1989, Nov-15, Volume: 264, Issue:32

    Topics: Acetates; Acetyl Coenzyme A; Acetylation; Animals; Chromatography, High Pressure Liquid; Coenzyme A; Cytidine Monophosphate N-Acetylneuraminic Acid; Diethyl Pyrocarbonate; Golgi Apparatus; Histidine; Intracellular Membranes; Kinetics; Liver; Lysine; Lysosomes; Models, Biological; Rats; Sialic Acids

1989
Specific chemical modifications of link protein and their effect on binding to hyaluronate and cartilage proteoglycan.
    Biochimica et biophysica acta, 1986, Mar-19, Volume: 881, Issue:1

    Topics: Acetylation; Animals; Arginine; Cartilage, Articular; Cattle; Chromatography, Gel; Diethyl Pyrocarbonate; Extracellular Matrix Proteins; Female; Histidine; Hyaluronic Acid; Imidazoles; Lysine; Male; Methylation; Proteins; Proteoglycans; Tryptophan; Tyrosine

1986
Chemical modification of 3 alpha,20 beta-hydroxysteroid dehydrogenase with diethyl pyrocarbonate. Evidence for an essential, highly reactive, lysyl residue.
    Biochemistry, 1987, Mar-10, Volume: 26, Issue:5

    Topics: Binding Sites; Binding, Competitive; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; Formates; Histidine; Hydrogen-Ion Concentration; Hydroxylamines; Hydroxysteroid Dehydrogenases; Lysine; NAD; Progesterone; Protein Conformation; Streptomyces

1987
Chemical modification of phenol hydroxylase by ethoxyformic anhydride.
    European journal of biochemistry, 1987, Dec-30, Volume: 170, Issue:1-2

    Topics: Diethyl Pyrocarbonate; Formates; Histidine; Kinetics; Lysine; Mathematics; Mixed Function Oxygenases; Pyridoxal Phosphate; Saccharomyces cerevisiae; Spectrophotometry

1987
Study of the role of histidyl, tyrosyl, alpha- or epsilon-amino residues in the specific binding of 3,5,3'-triiodothyronine to rat liver nuclear receptors.
    Acta endocrinologica, 1988, Volume: 117, Issue:2

    Topics: Amino Acids; Animals; Arginine; Cell Nucleus; Diethyl Pyrocarbonate; Histidine; Liver; Lysine; Male; Protein Conformation; Rats; Rats, Inbred Strains; Receptors, Thyroid Hormone; Triiodothyronine, Reverse; Trinitrobenzenesulfonic Acid; Tyrosine

1988
Chemical modification of the F0 part of the ATP synthase (F1F0) from Escherichia coli. Effects on proton conduction and F1 binding.
    European journal of biochemistry, 1984, Feb-01, Volume: 138, Issue:3

    Topics: Arginine; ATP Synthetase Complexes; Chemical Phenomena; Chemistry; Diethyl Pyrocarbonate; Escherichia coli; Liposomes; Lysine; Multienzyme Complexes; Peptide Fragments; Phosphotransferases; Protein Binding; Protons; Tyrosine

1984
Effect of chemical modification on the cryoprecipitation of monoclonal human cryoglobulin M.
    Biochimica et biophysica acta, 1984, Oct-23, Volume: 790, Issue:2

    Topics: Anisoles; Arginine; Cold Temperature; Cryoglobulins; Diethyl Pyrocarbonate; Formaldehyde; Histidine; Humans; Immunoglobulin M; Lysine; Protein Binding; Trinitrobenzenesulfonic Acid; Tryptophan; Tyrosine; Waldenstrom Macroglobulinemia

1984
Active-site covalent modifications of quinoprotein amine oxidases from Aspergillus niger. Evidence for binding of the mechanism-based inhibitor, 1,4-diamino-2-butyne, to residue Lys356 involved in the catalytic cycle.
    European journal of biochemistry, 1994, Nov-01, Volume: 225, Issue:3

    Topics: Amine Oxidase (Copper-Containing); Amino Acid Sequence; Aspergillus niger; Binding Sites; Diamines; Diethyl Pyrocarbonate; Kinetics; Lysine; Models, Chemical; Molecular Sequence Data; Phenylhydrazines; Schiff Bases; Sequence Homology, Amino Acid; Spectrophotometry; Substrate Specificity

1994
Inhibition of inositol 1,3,4-trisphosphate 5/6-kinase by amino acid modifying agents.
    Biochemical Society transactions, 1993, Volume: 21, Issue:4

    Topics: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Adenosine; Animals; Arginine; Benzenesulfonates; Brain; Diethyl Pyrocarbonate; Fluorescein-5-isothiocyanate; Histidine; Imidoesters; Indicators and Reagents; Lysine; Phosphotransferases (Alcohol Group Acceptor); Rose Bengal; Swine

1993
The effect of chemical modification of basic amino acid residues on the activation and amidolytic activity of Hageman factor (factor XII).
    The Journal of laboratory and clinical medicine, 1993, Volume: 122, Issue:6

    Topics: Amides; Amino Acids; Arginine; Diethyl Pyrocarbonate; Factor XII; Factor XIIa; Histidine; Humans; Lysine; Phenylglyoxal; Serum Albumin, Bovine; Structure-Activity Relationship; Trinitrobenzenesulfonic Acid

1993
Lysine 84 is at the subunit interface of lac repressor protein.
    The Journal of biological chemistry, 1993, Aug-15, Volume: 268, Issue:23

    Topics: Chromatography, Gel; Circular Dichroism; Diethyl Pyrocarbonate; Hydrogen Bonding; Immunoblotting; Kinetics; Lysine; Mutagenesis, Site-Directed; Operator Regions, Genetic; Protein Binding; Repressor Proteins

1993
Affinity radiolabeling identifies peptides and amino acids associated with substrate binding in human placental 3 beta-hydroxy-delta(5)-steroid dehydrogenase.
    The Journal of biological chemistry, 1993, Sep-05, Volume: 268, Issue:25

    Topics: 3-Hydroxysteroid Dehydrogenases; Affinity Labels; Alkylation; Amino Acid Sequence; Arginine; Binding Sites; Carbon Radioisotopes; Chromatography, High Pressure Liquid; Diethyl Pyrocarbonate; Female; Humans; Hydroxyprogesterones; Lysine; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Placenta; Pregnenolone; Sequence Analysis; Trypsin

1993
Chemical modification of bacteriophage T4 deoxynucleotide kinase. Evidence of a single catalytic region.
    The Journal of biological chemistry, 1993, Jan-25, Volume: 268, Issue:3

    Topics: Amino Acid Sequence; Bacteriophage T4; Binding Sites; Chromatography, High Pressure Liquid; Deoxycytidine Monophosphate; Deoxyguanine Nucleotides; Diethyl Pyrocarbonate; Enzyme Activation; Histidine; Kinetics; Lysine; Molecular Sequence Data; Nucleoside-Phosphate Kinase; Phosphorylation; Pyridoxal Phosphate; Substrate Specificity; Thymidine Monophosphate

1993
Site-directed mutagenesis and chemical modification of histidine residues on an alpha-class chick liver glutathione S-transferase CL 3-3. Histidines are not needed for the activity of the enzyme and diethylpyrocarbonate modifies both histidine and lysine
    European journal of biochemistry, 1993, Feb-01, Volume: 211, Issue:3

    Topics: Amino Acid Sequence; Animals; Baculoviridae; Base Sequence; Chickens; Diethyl Pyrocarbonate; Glutathione Transferase; Histidine; Hydrogen-Ion Concentration; Hydroxylamine; Hydroxylamines; Kinetics; Liver; Lysine; Molecular Sequence Data; Moths; Mutagenesis; Mutagenesis, Site-Directed; Peptide Mapping; Spectrophotometry; Trypsin

1993
Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.
    Biochemistry, 1996, Apr-09, Volume: 35, Issue:14

    Topics: Binding Sites; Chorismate Mutase; Circular Dichroism; Diethyl Pyrocarbonate; Enzyme Inhibitors; Escherichia coli; Histidine; Kinetics; Ligands; Lysine; Prephenate Dehydrogenase; Spectrometry, Fluorescence; Trinitrobenzenesulfonic Acid

1996
Evidence for an essential lysyl residue in phospholipase D from Streptomyces sp. by modification with diethyl pyrocarbonate and pyridoxal 5-phosphate.
    Biochemistry, 1996, Jul-30, Volume: 35, Issue:30

    Topics: Binding Sites; Circular Dichroism; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Kinetics; Lysine; Phospholipase D; Protein Conformation; Pyridoxal Phosphate; Streptomyces; Sulfhydryl Compounds

1996
Photodynamic crosslinking of proteins. II. Photocrosslinking of a model protein-ribonuclease A.
    Journal of photochemistry and photobiology. B, Biology, 1996, Volume: 35, Issue:3

    Topics: Animals; Cattle; Circular Dichroism; Cross-Linking Reagents; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; Histidine; Lysine; Photochemistry; Ribonuclease, Pancreatic

1996
Carbonyl reducing 17 beta-hydroxysteroid dehydrogenase from the filamentous fungus Cochliobolus lunatus.
    Advances in experimental medicine and biology, 1997, Volume: 414

    Topics: Alcohol Oxidoreductases; Aldehyde Reductase; Aldo-Keto Reductases; Androstenedione; Ascomycota; Binding Sites; Binding, Competitive; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Histidine; Hydroxysteroid Dehydrogenases; Immunoblotting; Kinetics; Lysine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet; Tetranitromethane; Trinitrobenzenesulfonic Acid; Tyrosine; Vitamin K

1997
Determination of diethylpyrocarbonate-modified amino acid residues in alpha 1-acid glycoprotein by high-performance liquid chromatography electrospray ionization-mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight-mass spectro
    Analytical biochemistry, 1998, Mar-15, Volume: 257, Issue:2

    Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Chymotrypsin; Diethyl Pyrocarbonate; Histidine; Hydrogen-Ion Concentration; Lysine; Molecular Sequence Data; Orosomucoid; Serine Endopeptidases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet; Trypsin; Tyrosine

1998
Studies on the amino acid residues of the active site of alpha-aspartyl dipeptidase.
    Annals of the New York Academy of Sciences, 1998, Dec-13, Volume: 864

    Topics: Arginine; Binding Sites; Catalytic Domain; Diethyl Pyrocarbonate; Dipeptidases; Escherichia coli; Kinetics; Lysine; Phenylglyoxal; Pyridoxal Phosphate; Salmonella typhimurium

1998
Chemical modification of NADP-isocitrate dehydrogenase from Cephalosporium acremonium evidence of essential histidine and lysine groups at the active site.
    European journal of biochemistry, 1999, Volume: 261, Issue:3

    Topics: Acremonium; Binding Sites; Diethyl Pyrocarbonate; Enzyme Inhibitors; Enzyme Reactivators; Histidine; Hydroxylamine; Isocitrate Dehydrogenase; Kinetics; Lysine; Pyridoxal Phosphate

1999
Chemical modification and site-directed mutagenesis of human liver arginase: evidence that the imidazole group of histidine-141 is not involved in substrate binding.
    Archives of biochemistry and biophysics, 1999, Nov-15, Volume: 371, Issue:2

    Topics: Arginase; Arginine; Catalytic Domain; Diethyl Pyrocarbonate; Histidine; Humans; Light; Liver; Lysine; Mutagenesis, Site-Directed; Ornithine; Rose Bengal

1999
Heparin-binding histidine and lysine residues of rat selenoprotein P.
    The Journal of biological chemistry, 2001, May-11, Volume: 276, Issue:19

    Topics: Amino Acid Sequence; Animals; Binding Sites; Carbon Radioisotopes; Chromatography, Affinity; Chromatography, High Pressure Liquid; Diethyl Pyrocarbonate; Heparin; Histidine; Hydrogen-Ion Concentration; Lysine; Molecular Sequence Data; Peptide Fragments; Proteins; Rats; Selenoprotein P; Selenoproteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2001
Conformation and polarity of the active site of xylanase I from Thermomonospora sp. as deduced by fluorescent chemoaffinity labeling. Site and significance of a histidine residue.
    European journal of biochemistry, 2001, Volume: 268, Issue:10

    Topics: Actinomycetales; Affinity Labels; Amino Acids; Binding Sites; Circular Dichroism; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Dose-Response Relationship, Drug; Enzyme Activation; Ethylmaleimide; Histidine; Hydroxymercuribenzoates; Indicators and Reagents; Kinetics; Lysine; Models, Chemical; o-Phthalaldehyde; Protease Inhibitors; Protein Binding; Protein Conformation; Sequence Analysis, Protein; Spectrometry, Fluorescence; Sulfhydryl Reagents; Time Factors; Trinitrobenzenesulfonic Acid; Urea; Xylan Endo-1,3-beta-Xylosidase; Xylosidases

2001
Site-directed mutagenesis of active site residues of phosphite dehydrogenase.
    Biochemistry, 2005, Mar-29, Volume: 44, Issue:12

    Topics: Amino Acid Sequence; Arginine; Bacterial Proteins; Binding Sites; Cysteine; Deuterium Exchange Measurement; Diethyl Pyrocarbonate; Glutamic Acid; Glutamine; Histidine; Hydrogen-Ion Concentration; Kinetics; Lysine; Molecular Sequence Data; Mutagenesis, Site-Directed; NADH, NADPH Oxidoreductases; Phosphites; Sequence Alignment

2005
Binding residues and catalytic domain of soluble Saccharomyces cerevisiae processing alpha-glucosidase I.
    Glycobiology, 2005, Volume: 15, Issue:12

    Topics: 1-Deoxynojirimycin; alpha-Glucosidases; Amino Acid Sequence; Binding Sites; Bromosuccinimide; Catalysis; Catalytic Domain; Diethyl Pyrocarbonate; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Enzyme Inhibitors; Gene Expression Regulation, Fungal; Glycoside Hydrolases; Glycosylation; Glyoxal; Histidine; Hydrolysis; Lysine; Molecular Sequence Data; Peptides; Phenylalanine; Protein Binding; Protein Structure, Tertiary; Saccharomyces cerevisiae; Temperature; Tetranitromethane; Time Factors; Trisaccharides; Trypsin; Tryptophan; Tyrosine

2005
Reactivity of histidine and lysine side-chains with diethylpyrocarbonate -- a method to identify surface exposed residues in proteins.
    Journal of biochemical and biophysical methods, 2008, Apr-24, Volume: 70, Issue:6

    Topics: Animals; Chickens; Diethyl Pyrocarbonate; Histidine; Horses; Humans; Lysine; Molecular Structure; Proteins; Spectrophotometry; Surface Properties

2008
Substrate-induced conformational changes in sarcoplasmic reticulum Ca2+-ATPase probed by surface modification using diethylpyrocarbonate with mass spectrometry.
    FEBS letters, 2012, Sep-21, Volume: 586, Issue:19

    Topics: Amino Acid Sequence; Animals; Catalytic Domain; Diethyl Pyrocarbonate; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; In Vitro Techniques; Lysine; Models, Molecular; Peptide Fragments; Protein Conformation; Protein Structure, Tertiary; Rabbits; Sarcoplasmic Reticulum; Sarcoplasmic Reticulum Calcium-Transporting ATPases; Spectrometry, Mass, Electrospray Ionization; Substrate Specificity; Tandem Mass Spectrometry

2012
Huntingtin N-Terminal Monomeric and Multimeric Structures Destabilized by Covalent Modification of Heteroatomic Residues.
    Biochemistry, 2015, Jul-21, Volume: 54, Issue:28

    Topics: Diethyl Pyrocarbonate; Humans; Huntingtin Protein; Lysine; Mass Spectrometry; Molecular Dynamics Simulation; Nerve Tissue Proteins; Peptides; Protein Conformation; Protein Multimerization; Protein Stability; Protein Structure, Secondary; Threonine

2015