diazeniumdiolate and diethylenetriamine

The prevalence of periodontal disease poses a significant global health burden. Treatments for these diseases, primarily focused on removal and eradication of dental plaque biofilms, are challenging due to limited access to periodontal pockets where these oral pathogens reside. Herein, we report on the development and characterization of nitric oxide (NO)-releasing carboxymethylcellulose (CMC) derivatives and evaluate their in vitro bactericidal efficacy against planktonic Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, two prominent periodontopathogens. Bactericidal exposure assays revealed that three of the synthesized NO-releasing polymers were capable of reducing bacterial viability of both species by 99.9% in 2 hr at concentrations of 4 mg ml

Topics: Aggregatibacter actinomycetemcomitans; Anti-Bacterial Agents; Azo Compounds; Biopolymers; Carboxymethylcellulose Sodium; Cell Line; Diamines; Drug Carriers; Drug Delivery Systems; Ethanolamines; Fibroblasts; Gingiva; Humans; Molecular Structure; Nitric Oxide; Periodontal Diseases; Polyamines; Porphyromonas gingivalis; Propylamines; Species Specificity; Viscosity

This study demonstrates the development of a nitric oxide (NO)-releasing hydrogel wound dressing and its efficacy at accelerating methicillin-resistant Staphylococcus aureus (MRSA)-infected wound healing. A DETA/NONOate-doped alginate (Alg-DETA/NO) hydrogel was synthesized using alginate as a hydrogel-forming wound dressing material and diethylenetriamine/diazeniumdiolate (DETA/NONOate) as an NO donor. Alg-DETA/NO exhibited a prolonged NO release profile over a period of 4 days. The rheological properties of Alg-DETA/NO did not differ significantly from those of pure alginate. Importantly, Alg-DETA/NO showed potent antibacterial activity against MRSA, with minimal toxicity to mouse fibroblasts. The application of Alg-DETA/NO to MRSA-infected wounds in a mouse model showed a favorable wound healing with accelerated wound-size reduction and reduced skin bacterial infection. Additionally, histological examination revealed that Alg-DETA/NO reduced inflammation at the wound site and promoted re-epithelialization, angiogenesis, and collagen deposition. Thus, Alg-DETA/NO presented herein could serve as a safe and potent hydrogel dressing for the treatment of MRSA-infected wounds.

Topics: Alginates; Animals; Anti-Bacterial Agents; Azo Compounds; Bandages; Collagen; Hydrogels; Methicillin-Resistant Staphylococcus aureus; Mice; Mice, Inbred ICR; Nitric Oxide; Nitric Oxide Donors; Peroxidase; Polyamines; Re-Epithelialization; Wound Healing