diazeniumdiolate has been researched along with alanosine* in 2 studies
2 other study(ies) available for diazeniumdiolate and alanosine
| Article | Year |
|---|---|
Reductases Produce Nitric Oxide in an Alternative Pathway to Form the Diazeniumdiolate Group of l-Alanosine.
l-Alanosine is a diazeniumdiolate ( Topics: Nitrate Reductases; Nitrates; Nitric Oxide; Nitrite Reductases; Oxidoreductases | 2023 |
The l-Alanosine Gene Cluster Encodes a Pathway for Diazeniumdiolate Biosynthesis.
N-Nitroso-containing natural products are bioactive metabolites with antibacterial and anticancer properties. In particular, compounds containing the diazeniumdiolate (N-nitrosohydroxylamine) group display a wide range of bioactivities ranging from cytotoxicity to metal chelation. Despite the importance of this structural motif, knowledge of its biosynthesis is limited. Herein we describe the discovery of a biosynthetic gene cluster in Streptomyces alanosinicus ATCC 15710 responsible for producing the diazeniumdiolate natural product l-alanosine. Gene disruption and stable isotope feeding experiments identified essential biosynthetic genes and revealed the source of the N-nitroso group. Additional biochemical characterization of the biosynthetic enzymes revealed that the non-proteinogenic amino acid l-2,3-diaminopropionic acid (l-Dap) is synthesized and loaded onto a free-standing peptidyl carrier protein (PCP) domain in l-alanosine biosynthesis, which we propose may be a mechanism of handling unstable intermediates generated en route to the diazeniumdiolate. These discoveries will facilitate efforts to determine the biochemistry of diazeniumdiolate formation. Topics: Alanine; Azo Compounds; Bacterial Proteins; Biosynthetic Pathways; Molecular Structure; Multigene Family; Streptomyces | 2020 |