diamide and dityrosine

diamide has been researched along with dityrosine* in 1 studies

Other Studies

1 other study(ies) available for diamide and dityrosine

Article	Year
On the cytotoxicity and status of oxidative stress of two novel synthesized tri-aza macrocyclic diamides as studied in the V79 cell lines. Bioorganic & medicinal chemistry, 2007, May-15, Volume: 15, Issue:10 Two tri-aza macrocycles as diamide derivatives of macrocyclic compounds possess a hydrophilic cavity surrounded by hydrophobic ring, which enables them to diffuse cell membrane and interfere with different living systems. In this study, we comparatively evaluated cytotoxicity effects of tri-aza dibenzo sulfoxide (TSD) and dibenzo sulfide (TTS) macrocyclic diamides in a range of doses (0.5-8mM) and the role of oxidative stress in V79 cell culture. We assessed the effects of these substances on ROS level, cellular viability, apoptosis events, activity of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and on some macromolecules' oxidative damage end-products: malondialdehyde (MDA), dityrosine, and 8-hydroxy-deoxyguanosine (8-OH-dG) that were assessed by spectrometry and HPLC methods. Both compounds revealed cytotoxicity effects on cell culture particularly at doses >1mM after 24-h incubation. They decreased cellular viability and significantly promoted ROS generation, increased enzyme activities, and enhanced oxidative damages in which TSD was more effective. Treatment of cells with each compound alone increased significantly the percent of apoptotic events at 2 and then 4mM. Co-treatment with alpha-tocopherol (alpha-TCP) drastically reduced these events. Cells' exposure with mixture of 30 microM alpha-tocopherol and 8mM of each compound exerted significant decrease in the levels of ROS, enzyme activities, and oxidative damage biomarkers. As conclusion, our study documented the oxidative radical forming ability of the studied compounds and further strengthened the documentation of their cytotoxicity effects through lipids, proteins and DNA oxidation damages. Topics: 8-Hydroxy-2'-Deoxyguanosine; Animals; Antineoplastic Agents; Antioxidants; Apoptosis; Catalase; Cell Line; Chromatography, High Pressure Liquid; Cricetinae; Cricetulus; Deoxyguanosine; Diamide; DNA Damage; Glutathione Peroxidase; Lipid Peroxidation; Macrocyclic Compounds; Malondialdehyde; Oxidative Stress; Proteins; Reactive Oxygen Species; Superoxide Dismutase; Tetrazolium Salts; Thiazoles; Tyrosine; Vitamin E	2007

Article

Year

On the cytotoxicity and status of oxidative stress of two novel synthesized tri-aza macrocyclic diamides as studied in the V79 cell lines.

Bioorganic & medicinal chemistry, 2007, May-15, Volume: 15, Issue:10

Two tri-aza macrocycles as diamide derivatives of macrocyclic compounds possess a hydrophilic cavity surrounded by hydrophobic ring, which enables them to diffuse cell membrane and interfere with different living systems. In this study, we comparatively evaluated cytotoxicity effects of tri-aza dibenzo sulfoxide (TSD) and dibenzo sulfide (TTS) macrocyclic diamides in a range of doses (0.5-8mM) and the role of oxidative stress in V79 cell culture. We assessed the effects of these substances on ROS level, cellular viability, apoptosis events, activity of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and on some macromolecules' oxidative damage end-products: malondialdehyde (MDA), dityrosine, and 8-hydroxy-deoxyguanosine (8-OH-dG) that were assessed by spectrometry and HPLC methods. Both compounds revealed cytotoxicity effects on cell culture particularly at doses >1mM after 24-h incubation. They decreased cellular viability and significantly promoted ROS generation, increased enzyme activities, and enhanced oxidative damages in which TSD was more effective. Treatment of cells with each compound alone increased significantly the percent of apoptotic events at 2 and then 4mM. Co-treatment with alpha-tocopherol (alpha-TCP) drastically reduced these events. Cells' exposure with mixture of 30 microM alpha-tocopherol and 8mM of each compound exerted significant decrease in the levels of ROS, enzyme activities, and oxidative damage biomarkers. As conclusion, our study documented the oxidative radical forming ability of the studied compounds and further strengthened the documentation of their cytotoxicity effects through lipids, proteins and DNA oxidation damages.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Animals; Antineoplastic Agents; Antioxidants; Apoptosis; Catalase; Cell Line; Chromatography, High Pressure Liquid; Cricetinae; Cricetulus; Deoxyguanosine; Diamide; DNA Damage; Glutathione Peroxidase; Lipid Peroxidation; Macrocyclic Compounds; Malondialdehyde; Oxidative Stress; Proteins; Reactive Oxygen Species; Superoxide Dismutase; Tetrazolium Salts; Thiazoles; Tyrosine; Vitamin E

2007