desmosterol has been researched along with 22-hydroxycholesterol* in 2 studies
2 other study(ies) available for desmosterol and 22-hydroxycholesterol
| Article | Year |
|---|---|
HPLC method for quantification and characterization of cholesterol and its oxidation products in eggs.
A new method was developed for the simultaneous determination of cholesterol and its oxidation products in eggs, using HPLC with UV and refractive index (RI) detectors, and HPLC interfaced with atmospheric pressure chemical ionization coupled to MS (HPLC-APCI-MS). The best conditions for direct saponification of the sample and extraction of the non-saponifiable material were defined using complete factorial designs with central points. The method showed accuracy and precision with a detection limit between 0.002 and 0.079 microg/g. The oxides cholest-5-ene-3beta,20alpha-diol and cholest-5-ene-3beta,25-diol identified by HPLC-UV-RI were not confirmed by HPLC-APCI-MS. Topics: Air Ionization; Atmospheric Pressure; Cholesterol; Chromatography, High Pressure Liquid; Chromatography, Liquid; Desmosterol; Eggs; Food Analysis; Hydroxycholesterols; Ketocholesterols; Mass Spectrometry; Oxidation-Reduction; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Ultraviolet Rays | 2006 |
Effects of 22S-hydroxy-cholesterol and other hydroxylated sterols on the ACTH-stimulated steroid production in rat adrenal cells.
Several effects of hydroxylated sterols on cell cultures are known. Most of these can be explained by an inhibition of the cholesterol synthesis at the level of the 3-hydroxy-3-methylglutaryl CoA reductase. When studying cholesterol metabolism in rat adrenal cells, an inhibitory action of some sterols on the ACTH-stimulated corticosterone production was observed. The effects of one sterol, 22S-OH-cholesterol, were investigated further. The sterol had no effect on the ACTH-stimulated cyclic AMP production, suggesting an intact receptor-adenylate cyclase complex and cellular membrane. In the presence of ACTH and 22-OH-cholesterol particularly the free cholesterol concentration was elevated; 22S-OH-cholesterol therefore probably exerts its inhibitory effect at a step located after hydrolysis of the cholesterol esters. 22S-OH-cholesterol had no effect on the conversion of exogenous pregnenolone into corticosterone. These results make it probable, that the inhibitory effect of 22S-OH-cholesterol on the ACTH-stimulated corticosterone production is situated at the level of the cholesterol side-chain cleavage. Topics: Adrenal Glands; Adrenocorticotropic Hormone; Animals; Cholestadienols; Cholesterol; Corticosterone; Cyclic AMP; Desmosterol; Hydroxycholesterols; Ketocholesterols; Male; Pregnenolone; Rats; Sterols | 1981 |