deoxy-4-thiothymidine and cupric-chloride

deoxy-4-thiothymidine has been researched along with cupric-chloride* in 1 studies

Other Studies

1 other study(ies) available for deoxy-4-thiothymidine and cupric-chloride

ArticleYear
Site-specific modification of the 6-amino group of adenosine in RNA by an interstrand functionality-transfer reaction with an s-functionalized 4-thiothymidine.
    Chembiochem : a European journal of chemical biology, 2015, May-26, Volume: 16, Issue:8

    Non-natural RNA modifications have been widely used to study the function and structure of RNA. Expanding the study of RNA further requires versatile and efficient tools for site-specific RNA modification. We recently established a new strategy for the site-specific modification of RNA based on a functionality-transfer reaction between an oligodeoxynucleotide (ODN) probe and an RNA substrate. 2'-Deoxy-6-thioguanosine was used to anchor the transfer group, and the 4-amino group of cytosine or the 2-amino group of guanine was specifically modified. In this study, 2'-deoxy-4-thiothymidine was adopted as a new platform to target the 6-amino group of adenosine. The (E)-pyridinyl vinyl keto transfer group was attached to the 4-thioT in the ODN probe, and it was efficiently and specifically transferred to the 6-amino group of the opposing adenosine in RNA in the presence of CuCl2 . This method expands the available RNA target sites for specific modification.

    Topics: Adenosine; Base Sequence; Binding Sites; Copper; Oligodeoxyribonucleotides; RNA; Sulfur; Thymidine

2015