deltoin and imperatorin

To investigate the chemical constituents of Peucedanum decursivum commonly used in traditional Chinese medicine.. Compounds were separated by solvent extraction and column chromatography with silica gel and structures were elucidated by chemical evidence and spectral analysis.. Seven compounds were identified as imperatorin, deltoin, columbianadin, beta-sitosterol, scopoletin, (+)-trans-decursidinol and daucosterol.. All the compounds were isolated from the plant for the first time.

Topics: Apiaceae; Coumarins; Furocoumarins; Plant Leaves; Plant Stems; Plants, Medicinal; Scopoletin

L-Arginine derived nitric oxide (NO) and its derivatives, such as nitrogen dioxide and peroxynitrite, play a role in inflammation and also possibly in the multistage process of carcinogenesis. Four furanocoumarins and eight chromones isolated from the dried root of Saposhnikovia divaricata (Fang Feng in Chinese) and evaluated for their effects on the synthesis of NO induced by lipopolysaccharide (LPS) in macrophage cell line RAW 264.7. The inhibition of nitrite production, as an index for NO released from the macrophage cells, was quantitatively analyzed by Griess reaction. The results showed that imperatorin and deltoin are potential NO production inhibitor, and their IC50 values for inhibition of nitrite production were 17.3 and 11.6 microg/ml, respectively. Western-blot analysis demonstrated that iNOS enzyme activity was not inhibited by treatment with imperatorin or deltoin, but revealed that both compounds inhibited the expression of the iNOS protein.

Topics: Animals; Cell Line; Cytosol; Drugs, Chinese Herbal; Enzyme Inhibitors; Furocoumarins; Lipopolysaccharides; Macrophages; Mice; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Plant Roots