deacetylcytochalasin-h and alternariol

Chemical investigation of an acetonitrile fraction from the endophytic fungus Phomopsis sp. led to the isolation of the new natural product 2-hydroxy-alternariol (7) together with the known compounds cytochalasins J (1) and H (2), 5'-epialtenuene (3) and the mycotoxins alternariol monomethyl ether (AME, 4), alternariol (AOH, 5) and cytosporone C (6). The structure of the new compound was elucidated by using 1-D and 2-D NMR (nuclear magnetic resonance) and high resolution mass spectrometry. The cytochalasins J (1) and H (2) and AOH (5) exhibited potent inhibition of the total ROS (reactive oxygen species) produced by stimulated human neutrophils and acted as potent potential anti-inflammatory agents. Moreover, cytochalasin H (2) demonstrated antifungal and acetylcholinesterase enzyme (AChE) inhibition in vitro.

Topics: Anti-Inflammatory Agents, Non-Steroidal; Antifungal Agents; Antioxidants; Ascomycota; Cholinesterase Inhibitors; Cytochalasins; Drug Evaluation, Preclinical; Endophytes; Humans; Lactones; Magnetic Resonance Spectroscopy; Molecular Structure; Mycotoxins; Neutrophils; Reactive Oxygen Species; Secondary Metabolism; Senna Plant; Sesquiterpenes