d-609 and aluminum-tetrasulfophthalocyanine

1. Photodynamic agents, due to their photon-dependent selective activation, can selectively activate a number of physiological processes and may directly modulate signal transduction in a number of cells including pancreatic acinar cells. 2. Activation of the photodynamic agent sulphonated aluminium phthalocyanine (SALPC) triggered recurrent cytosolic calcium ([Ca2+]i) spiking in pancreatic acinar cells. 3. The photodynamically triggered calcium spiking could be blocked by phosphatidylinositol-specific phospholipase C (PI-PLC) inhibitor U73122, but not by phosphatidylcholine-specific phospholipase C inhibitor D609. 4. Removal of extracellular Ca2+ abolished spiking, as did 2-aminoethoxydiphenylborate (2-APB), an inhibitory modulator of IP3-mediated Ca2+ release from intracellular stores. 5. These data suggest that SALPC photodynamic action may permanently fix PI-PLC in an active conformation, and this produced recurrent [Ca2+]i spiking.

Topics: Animals; Bridged-Ring Compounds; Calcium; Enzyme Inhibitors; Estrenes; In Vitro Techniques; Indoles; Male; Norbornanes; Organometallic Compounds; Pancreas; Phosphodiesterase Inhibitors; Photic Stimulation; Pyrrolidinones; Rats; Rats, Sprague-Dawley; Thiocarbamates; Thiones; Type C Phospholipases