cytochrome-c-t and troxerutin

Mitochondrial oxidative stress plays a major role in the pathogenesis of myocardial apoptosis in metabolic syndrome (MS) patients. In this study, we investigated the effect of troxerutin (TX), an antioxidant on mitochondrial oxidative stress and apoptotic markers in heart of mice fed fat and fructose-rich diet. Adult male Mus musculus mice were fed either control diet or high fat, high fructose diet (HFFD) for 60 days to induce MS. Mice from each dietary group were divided into two on the 16th day and were either treated or untreated with TX (150 mg/kg bw, p.o) for the next 45 days. At the end of the study, mitochondrial reactive oxygen species (ROS) generation, oxidative stress markers, levels of intracellular calcium, cardiolipin content, cytochrome c release and apoptotic markers were examined in the myocardium. HFFD-feeding resulted in diminution of antioxidants and increased ROS production, lipid peroxidation and oxidatively modified adducts of 8-OHG, 4-HNE and 3-NT. Further increase in Ca

Topics: Animals; Antioxidants; Apoptosis; bcl-2-Associated X Protein; Calcium; Cardiolipins; Caspase 3; Caspase 9; Cytochromes c; Diet, High-Fat; DNA Adducts; Fructose; Heart; Hydroxyethylrutoside; Mice; Mitochondria; Myocardium; Oxidative Stress; Proto-Oncogene Proteins c-bcl-2; Reactive Oxygen Species