cytochrome-c-t has been researched along with thiochrome* in 1 studies
1 other study(ies) available for cytochrome-c-t and thiochrome
| Article | Year |
|---|---|
Fluorometry detection for trypsin via inner filter effect between cytochrome C and in-situ formed fluorescent thiochrome.
A fluorometry assay for trypsin sensitive determination has been presented. The fluorescence of the system at 370/445 nm is derived from thiochrome obtained by in-situ oxidation of thiamine. Based on the inner filter effect, cytochrome C (Cyt C) can quench the fluorescence at 445 nm effectively. Cyt C is specifically hydrolyzed by trypsin through an enzymatic reaction, giving rise to the enhancement of the fluorescence intensity. The change value of fluorescence intensity is proportional to trypsin concentration, which is successfully used for trypsin quantitative detection. This method exhibits good repeatability and selectivity with a detection limit of 0.15 μg mL Topics: Cytochromes c; Fluorescent Dyes; Fluorometry; Humans; Thiamine; Trypsin | 2021 |