cytochrome-c-t and tabersonine

Methyl jasmonate, a chemical inducer of secondary metabolism, was shown to promote tabersonine 2 biosynthesis in hairy root cultures of Catharanthus roseus. Tabersonine 6,7-epoxidase activity was detected in total protein extract of jasmonate-induced hairy root cultures using labeled 14C-tabersonine 2. This enzyme converted tabersonine 2 to lochnericine 3 by selective epoxidation at positions 6 and 7 via a reaction dependent on NADPH and molecular oxygen. Carbon monoxide, clotrimazole, miconazole, and cytochrome C were shown to be strong inhibitors of the enzyme. The activity was found in microsomes, indicating that tabersonine 6,7-epoxidase was a cytochrome P-450-dependent monooxygenase.

Topics: Acetates; Alkaloids; Carbon Monoxide; Catharanthus; Cyclopentanes; Cytochrome P-450 Enzyme System; Cytochromes c; Enzyme Inhibitors; Epoxy Compounds; Hydrolases; Indole Alkaloids; Indoles; Microsomes; NADP; Oxidation-Reduction; Oxidoreductases; Oxygen; Oxylipins; Plant Roots; Quinolines