cytochrome-c-t and naringin

Naringin is a flavonoid which has a therapeutic effect. However, the details of its antifungal mechanism have not yet been fully elucidated. This study focused on clarifying the relationship between naringin and Candida albicans, to understand its mode of antifungal action. In general, naringin is an antioxidant, but our results indicated that 1 mM naringin generates intracellular superoxide (O

Topics: Antifungal Agents; Calcium; Candida albicans; Caspases; Cytochromes c; DNA Damage; Flavanones; Glutathione; Membrane Potential, Mitochondrial; Microbial Sensitivity Tests; Mitochondria; Reactive Oxygen Species

Naringin, the primary active compound of the traditional Chinese medicine Rhizoma drynariae, possesses many pharmacological activities. The present study is an effort to explore the anti-osteoporosis potential of naringin in vivo and in vitro. In vivo, we used ovariectomized rats to clarify the mechanisms by which naringin anti-osteoporosis. In vitro, we used osteoclasts to investigate naringin promotes osteoclasts apoptosis. Naringin was effective at enhancing BMD, trabecular thickness, bone mineralization, and mechanical strength in a dose-dependent manner. The result of RT-PCR analysis revealed that naringin down-regulated the mRNA expression levels of BCL-2 and up-regulated BAX, caspase-3 and cytochrome C. In addition, naringin significantly reduced the bone resorption area in vitro. These findings suggest that naringin promotes the apoptosis of osteoclasts by regulating the activity of the mitochondrial apoptosis pathway and prevents OVX-induced osteoporosis in rats.

Topics: Animals; Apoptosis; Bone Density; Bone Density Conservation Agents; Bone Resorption; Calcification, Physiologic; Caspase 3; Cell Line; Cytochromes c; Dose-Response Relationship, Drug; Female; Flavanones; Gene Expression; Humans; Mitochondria; Osteoclasts; Osteoporosis; Ovariectomy; Plant Extracts; Proto-Oncogene Proteins c-bcl-2; Rats; Rats, Sprague-Dawley