cytochrome-c-t and indirubin

Zucker fatty rats were subjected to warm ischaemia and 12 hours of reperfusion. Ind or NAD. Pretreatment decreased markers of liver injury while preserving mitochondrial cytochrome c content, which is related to the prevention of calcium-induced mitochondrial permeability transition (mPT), the decline in mitochondrial respiratory state 3 and ATP content. The generation of reactive oxygen species (ROS) was also diminished. Inhibition of GSK-3ß by Ind resulted in the prevention of cyclophilin-D (CypD) phosphorylation, unabling it to bind to the adenine nucleotide translocator (ANT), thus, preventing mPT induction. Furthermore, deacetylation of CypD at Lys residue by sirtuin 3 (SIRT3) caused its dissociation from ANT, contributing to an increase in mPT threshold in NAD

Topics: Adenosine Triphosphate; Animals; Antibiotics, Antineoplastic; Calcium; Cyclophilins; Cytochromes c; Fatty Liver; Glycogen Synthase Kinase 3 beta; Hepatic Artery; Indoles; Liver; Mitochondria, Liver; Mitochondrial ADP, ATP Translocases; NAD; Oximes; Peptidyl-Prolyl Isomerase F; Rats; Rats, Zucker; Reactive Oxygen Species; Reperfusion Injury; Sirtuins; Warm Ischemia