cytochrome-c-t and ferric-phosphate

cytochrome-c-t has been researched along with ferric-phosphate* in 1 studies

Other Studies

1 other study(ies) available for cytochrome-c-t and ferric-phosphate

Article	Year
Fluoridized iron phosphate as a novel adsorbent for selective separation/isolation of cytochrome c. Analytical and bioanalytical chemistry, 2011, Volume: 401, Issue:10 Fluoridized iron phosphate (F-FePO) is prepared via a hydrothermal protocol and characterized by means of (57)Fe Mössbauer spectra, Fourier transform infrared, and surface charge analysis. For the first time, F-FePO is used as an adsorbent for the adsorption of proteins, which exhibits favorable selectivity toward cytochrome c (cyt-c) in the presence of acidic and neutral proteins under controlled experimental conditions. At pH 10.5, an adsorption efficiency of 100% is achieved for 60 mg L(-1) cyt-c in 1.0 mL of sample solution using 6.0 mg F-FePO. The adsorption behavior is consistent with the Langmuir adsorption model, corresponding to a theoretical adsorption capacity of 37.59 μg mg(-1). The retained cyt-c on F-FePO could be readily collected by 0.1 mol L(-1) Na(2)CO(3)-NaHCO(3) buffer (pH 10.5), deriving a recovery of 100%. Circular dichroism spectra indicate no conformational change for cyt-c after the adsorption and desorption processes, demonstrating the favorable biocompatibility of the fluoridized iron phosphate. F-FePO is employed for the selective isolation of cyt-c from a spiked human whole blood, achieving satisfactory results by assay with SDS-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis. Topics: Adsorption; Cytochromes c; Ferric Compounds; Humans; Hydrogen-Ion Concentration; Solid Phase Extraction	2011

Article

Year

Fluoridized iron phosphate as a novel adsorbent for selective separation/isolation of cytochrome c.

Analytical and bioanalytical chemistry, 2011, Volume: 401, Issue:10

Fluoridized iron phosphate (F-FePO) is prepared via a hydrothermal protocol and characterized by means of (57)Fe Mössbauer spectra, Fourier transform infrared, and surface charge analysis. For the first time, F-FePO is used as an adsorbent for the adsorption of proteins, which exhibits favorable selectivity toward cytochrome c (cyt-c) in the presence of acidic and neutral proteins under controlled experimental conditions. At pH 10.5, an adsorption efficiency of 100% is achieved for 60 mg L(-1) cyt-c in 1.0 mL of sample solution using 6.0 mg F-FePO. The adsorption behavior is consistent with the Langmuir adsorption model, corresponding to a theoretical adsorption capacity of 37.59 μg mg(-1). The retained cyt-c on F-FePO could be readily collected by 0.1 mol L(-1) Na(2)CO(3)-NaHCO(3) buffer (pH 10.5), deriving a recovery of 100%. Circular dichroism spectra indicate no conformational change for cyt-c after the adsorption and desorption processes, demonstrating the favorable biocompatibility of the fluoridized iron phosphate. F-FePO is employed for the selective isolation of cyt-c from a spiked human whole blood, achieving satisfactory results by assay with SDS-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis.

Topics: Adsorption; Cytochromes c; Ferric Compounds; Humans; Hydrogen-Ion Concentration; Solid Phase Extraction

2011