cytochrome-c-t and dithiobis(succinimidylpropionate)

cytochrome-c-t has been researched along with dithiobis(succinimidylpropionate)* in 1 studies

Other Studies

1 other study(ies) available for cytochrome-c-t and dithiobis(succinimidylpropionate)

Article	Year
Detection of the superoxide radical anion using various alkanethiol monolayers and immobilized cytochrome c. Analytical chemistry, 2008, Dec-15, Volume: 80, Issue:24 The superoxide radical anion (SO) is a critical biomarker for monitoring cellular stress responses. Electrochemical SO biosensors are frequently constructed through the covalent immobilization of cytochrome c (Cyt c) onto self-assembled monolayers (SAMs); however, a detailed comparison of these systems as well as configuration influence on SO detection is needed to enable robust applications. Two reaction pathways, oxidation of SO by the SAM-modified gold electrode or electron transfer through a protein and monolayer relay, may be involved during the electrochemical detection of SO with Cyt c, depending on the SAM that is used. Although electrodes with SAMs alone can exhibit a high sensitivity and low limit of detection (LOD) for the SO, they can suffer from a strong response to the presence of interferents such as hydrogen peroxide and ascorbic acid. Electrodes with immobilized Cyt c show decreased sensitivity, but exhibit better selectivity and resistance to fouling in complex media. Considering the trade-offs between sensitivity, selectivity, and LOD for SO detection, a bioelectrode made with Cyt c immobilized on dithiobis(succinimidyl)propionate (DTSP) appears to be the most suitable configuration. In phosphate buffer, the DTSP/Cyt c electrode has a sensitivity of 410 nA microM(-1) cm(-2) and an LOD for SO of 73 nM. Results are also presented for the detection of SO in a complex tissue culture media (MEM) with and without serum, and the sensitivity of the DTSP/Cyt c in MEM in the absence of serum increased to 640 nA microM(-1) cm(-2). By measuring SO with a DTSP/Cyt c electrode before and after the addition of a bolus of the superoxide dismutase (SOD) enzyme, the specificity of the SOD enzyme can be combined with the sensitivity of Cyt c system. Topics: Animals; Biosensing Techniques; Catalysis; Cattle; Cytochromes c; Electrochemistry; Electrodes; Enzymes, Immobilized; Micro-Electrical-Mechanical Systems; Succinimides; Superoxide Dismutase; Superoxides; Xanthine; Xanthine Oxidase	2008

Article

Year

Detection of the superoxide radical anion using various alkanethiol monolayers and immobilized cytochrome c.

Analytical chemistry, 2008, Dec-15, Volume: 80, Issue:24

The superoxide radical anion (SO) is a critical biomarker for monitoring cellular stress responses. Electrochemical SO biosensors are frequently constructed through the covalent immobilization of cytochrome c (Cyt c) onto self-assembled monolayers (SAMs); however, a detailed comparison of these systems as well as configuration influence on SO detection is needed to enable robust applications. Two reaction pathways, oxidation of SO by the SAM-modified gold electrode or electron transfer through a protein and monolayer relay, may be involved during the electrochemical detection of SO with Cyt c, depending on the SAM that is used. Although electrodes with SAMs alone can exhibit a high sensitivity and low limit of detection (LOD) for the SO, they can suffer from a strong response to the presence of interferents such as hydrogen peroxide and ascorbic acid. Electrodes with immobilized Cyt c show decreased sensitivity, but exhibit better selectivity and resistance to fouling in complex media. Considering the trade-offs between sensitivity, selectivity, and LOD for SO detection, a bioelectrode made with Cyt c immobilized on dithiobis(succinimidyl)propionate (DTSP) appears to be the most suitable configuration. In phosphate buffer, the DTSP/Cyt c electrode has a sensitivity of 410 nA microM(-1) cm(-2) and an LOD for SO of 73 nM. Results are also presented for the detection of SO in a complex tissue culture media (MEM) with and without serum, and the sensitivity of the DTSP/Cyt c in MEM in the absence of serum increased to 640 nA microM(-1) cm(-2). By measuring SO with a DTSP/Cyt c electrode before and after the addition of a bolus of the superoxide dismutase (SOD) enzyme, the specificity of the SOD enzyme can be combined with the sensitivity of Cyt c system.

Topics: Animals; Biosensing Techniques; Catalysis; Cattle; Cytochromes c; Electrochemistry; Electrodes; Enzymes, Immobilized; Micro-Electrical-Mechanical Systems; Succinimides; Superoxide Dismutase; Superoxides; Xanthine; Xanthine Oxidase

2008