cytochrome-c-t and corrole

The tumor-targeted corrole particle, HerGa, displays preferential toxicity to tumors in vivo and can be tracked via fluorescence for simultaneous detection, imaging, and treatment. We have recently uncovered an additional feature of HerGa in that its cytotoxicity is enhanced by light irradiation. In the present study, we have elucidated the cellular mechanisms for HerGa photoexcitation-mediated cell damage using fluorescence optical imaging. In particular, we found that light irradiation of HerGa produces singlet oxygen, causing mitochondrial damage and cytochrome c release, thus promoting apoptotic cell death. An understanding of the mechanisms of cell death induced by HerGa, particularly under conditions of light-mediated excitation, may direct future efforts in further customizing this nanoparticle for additional therapeutic applications and enhanced potency.

Topics: Antineoplastic Agents; Cell Line, Tumor; Cell Survival; Cytochromes c; Humans; Light; Membrane Potential, Mitochondrial; Mitochondria; Nanoparticles; Neoplasms; Porphyrins; Singlet Oxygen

The first report regarding SOD activity of metallocorroles, investigated via the combination of the cytochrome C assay, pulse radiolysis, and electrochemistry, is used for identifying the main criteria needed for achieving good performance, as well as for elucidating mechanistic aspects of their action.

Topics: Cytochromes c; Metals; Oxidation-Reduction; Porphyrins; Sulfonic Acids; Superoxide Dismutase