cytochrome-c-t and bathophenanthroline

cytochrome-c-t has been researched along with bathophenanthroline* in 1 studies

Other Studies

1 other study(ies) available for cytochrome-c-t and bathophenanthroline

Article	Year
Iron regulation through the back door: iron-dependent metabolite levels contribute to transcriptional adaptation to iron deprivation in Saccharomyces cerevisiae. Eukaryotic cell, 2010, Volume: 9, Issue:3 Budding yeast (Saccharomyces cerevisiae) responds to iron deprivation both by Aft1-Aft2-dependent transcriptional activation of genes involved in cellular iron uptake and by Cth1-Cth2-specific degradation of certain mRNAs coding for iron-dependent biosynthetic components. Here, we provide evidence for a novel principle of iron-responsive gene expression. This regulatory mechanism is based on the modulation of transcription through the iron-dependent variation of levels of regulatory metabolites. As an example, the LEU1 gene of branched-chain amino acid biosynthesis is downregulated under iron-limiting conditions through depletion of the metabolic intermediate alpha-isopropylmalate, which functions as a key transcriptional coactivator of the Leu3 transcription factor. Synthesis of alpha-isopropylmalate involves the iron-sulfur protein Ilv3, which is inactivated under iron deficiency. As another example, decreased mRNA levels of the cytochrome c-encoding CYC1 gene under iron-limiting conditions involve heme-dependent transcriptional regulation via the Hap1 transcription factor. Synthesis of the iron-containing heme is directly correlated with iron availability. Thus, the iron-responsive expression of genes that are downregulated under iron-limiting conditions is conferred by two independent regulatory mechanisms: transcriptional regulation through iron-responsive metabolites and posttranscriptional mRNA degradation. Only the combination of the two processes provides a quantitative description of the response to iron deprivation in yeast. Topics: 3-Isopropylmalate Dehydrogenase; CCAAT-Binding Factor; Ceruloplasmin; Cytochromes c; DNA-Binding Proteins; Down-Regulation; Ferrochelatase; Gene Expression; Gene Expression Regulation, Fungal; Heme; Homeostasis; Hydro-Lyases; Iron; Iron Chelating Agents; Iron Deficiencies; Isomerases; Malates; Peroxidases; Phenanthrolines; Promoter Regions, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Terminator Regions, Genetic; Trans-Activators; Transcription Factors; Tristetraprolin; Up-Regulation	2010

Article

Year

Iron regulation through the back door: iron-dependent metabolite levels contribute to transcriptional adaptation to iron deprivation in Saccharomyces cerevisiae.

Eukaryotic cell, 2010, Volume: 9, Issue:3

Budding yeast (Saccharomyces cerevisiae) responds to iron deprivation both by Aft1-Aft2-dependent transcriptional activation of genes involved in cellular iron uptake and by Cth1-Cth2-specific degradation of certain mRNAs coding for iron-dependent biosynthetic components. Here, we provide evidence for a novel principle of iron-responsive gene expression. This regulatory mechanism is based on the modulation of transcription through the iron-dependent variation of levels of regulatory metabolites. As an example, the LEU1 gene of branched-chain amino acid biosynthesis is downregulated under iron-limiting conditions through depletion of the metabolic intermediate alpha-isopropylmalate, which functions as a key transcriptional coactivator of the Leu3 transcription factor. Synthesis of alpha-isopropylmalate involves the iron-sulfur protein Ilv3, which is inactivated under iron deficiency. As another example, decreased mRNA levels of the cytochrome c-encoding CYC1 gene under iron-limiting conditions involve heme-dependent transcriptional regulation via the Hap1 transcription factor. Synthesis of the iron-containing heme is directly correlated with iron availability. Thus, the iron-responsive expression of genes that are downregulated under iron-limiting conditions is conferred by two independent regulatory mechanisms: transcriptional regulation through iron-responsive metabolites and posttranscriptional mRNA degradation. Only the combination of the two processes provides a quantitative description of the response to iron deprivation in yeast.

Topics: 3-Isopropylmalate Dehydrogenase; CCAAT-Binding Factor; Ceruloplasmin; Cytochromes c; DNA-Binding Proteins; Down-Regulation; Ferrochelatase; Gene Expression; Gene Expression Regulation, Fungal; Heme; Homeostasis; Hydro-Lyases; Iron; Iron Chelating Agents; Iron Deficiencies; Isomerases; Malates; Peroxidases; Phenanthrolines; Promoter Regions, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Terminator Regions, Genetic; Trans-Activators; Transcription Factors; Tristetraprolin; Up-Regulation

2010