cytochrome-c-t and alcohol-oxidase

In this work, a new electrochemical biosensor based on catalyzed hairpin assembly target recycling and cascade electrocatalysis (cytochrome c (Cyt c) and alcohol oxidase (AOx)) for signal amplification was constructed for highly sensitive detection of microRNA (miRNA). It is worth pointing out that target recycling was achieved only based on strand displacement process without the help of nuclease. Moreover, porous TiO2 nanosphere was synthesized, which could offer more surface area for Pt nanoparticles (PtNPs) enwrapping and enhance the amount of immobilized DNA strand 1 (S1) and Cyt c accordingly. With the mimicking sandwich-type reaction, the cascade catalysis amplification strategy was carried out by AOx catalyzing ethanol to acetaldehyde with the concomitant formation of high concentration of H2O2, which was further electrocatalyzed by PtNPs and Cyt c. This newly designed biosensor provided a sensitive detection of miRNA-155 from 0.8 fM to 1 nM with a relatively low detection limit of 0.35 fM.

Topics: Acetaldehyde; Alcohol Oxidoreductases; Aptamers, Nucleotide; Base Sequence; Biocompatible Materials; Biosensing Techniques; Catalysis; Citric Acid; Cytochromes c; Electrochemistry; Humans; Hydrogen Peroxide; MicroRNAs; Molecular Sequence Data; Nanoparticles; Oxidation-Reduction; Porosity; Reproducibility of Results; Signal Processing, Computer-Assisted; Titanium