cytochrome-c-t and 6-mercapto-1-hexanol

A novel and versatile method, based on a membrane-free enzyme electrode in which both the enzyme and a mediator protein are entrapped in a gelatine hydrogel was developed for the fabrication of biosensors. As a proof of principle, we prepared a hydrogen peroxide biosensor by successfully entrapping both horse heart cytochrome c (HHC) and Saccharomyces cerevisae cytochrome c peroxidase (CCP) in a gelatin matrix which is immobilized on a gold electrode. This electrode was first pretreated with 6-mercaptohexanol. The biosensor displayed a rapid response and an expanded linear response range from 0 to 0.3 mM (R = 0.987) with a detection limit of 1 × 10(-5)M in a HEPES buffer solution (pH 7.0). This method of encapsulation is now further investigated for industrial biosensor applications.

Topics: Animals; Biosensing Techniques; Cytochrome-c Peroxidase; Cytochromes c; Electrochemistry; Electrodes; Enzymes, Immobilized; Gelatin; Gold; Hexanols; Horses; Hydrogen Peroxide; Hydrogen-Ion Concentration; Limit of Detection; Potentiometry; Saccharomyces cerevisiae; Sulfhydryl Compounds