cytochrome-c-t and 2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid

The determination of cytochrome c in the human serum sample is a regular medical investigation performed to assess cancer diseases. Herein, we used interferometric reflectance spectroscopy (IRS) based biosensor for the determination of cytochrome c. For this purpose first, the nanoporous anodic alumina (NAA) was fabricated. Then, the NAA pore walls were functionalized with 3-aminopropyl trimethoxy silane (NAA-NH

Topics: Aluminum Oxide; Benzothiazoles; Biosensing Techniques; Cytochromes c; Humans; Hydrogen Peroxide; Interferometry; Nanopores; Neoplasms; Spectrum Analysis; Sulfonic Acids; Trypsin

The encapsulation of enzymes within porous materials has shown great promise, not only in protecting the enzymes from denaturation under nonbiological environments, but also, in some cases, in facilitating their enzymatic reaction rates at favorable reaction conditions. While a number of hypotheses have been developed to explain this phenomenon, the detailed structural changes of the enzymes upon encapsulation within the porous material, which are closely related to their activity, remain largely elusive. Herein, the structural change of cytochrome c (Cyt c) upon encapsulation within a hierarchical metal-organic framework, NU-1000, is investigated through a combination of experimental and computational methods, such as electron paramagnetic resonance, solid-state ultraviolet-visible spectroscopy, and all-atom explicit solvent molecular dynamics simulations. The enhanced catalytic performance of Cyt c after being encapsulated within NU-1000 is supported by the physical and in silico observations of a change around the heme ferric active center.

Topics: Benzothiazoles; Biocatalysis; Catalytic Domain; Cytochromes c; Density Functional Theory; Heme; Metal-Organic Frameworks; Molecular Dynamics Simulation; Oxidation-Reduction; Spectrophotometry; Sulfonic Acids