cytochrome-c-t and 2--deoxy-5--adenosine-monophosphate

cytochrome-c-t has been researched along with 2--deoxy-5--adenosine-monophosphate* in 1 studies

Other Studies

1 other study(ies) available for cytochrome-c-t and 2--deoxy-5--adenosine-monophosphate

Article	Year
Deoxy-adenosine-monophosphate (dAMP) di-n-butylester induces apoptosis by increasing the dATP level in HL-60 cells. Cancer letters, 2006, Apr-28, Volume: 235, Issue:2 Deoxy-ATP is a potent inducer of apoptosis. We intended to synthesize a lipophilic dAMP derivative which, according to our working hypothesis penetrates into the cell, is converted to dAMP by intracellular esterases and to dATP by nucleotide kinases. We synthesized dAMP-di-n-butylester (DAB) and tested it. We found that it fulfills the above-described expectations. DAB treatment decreases the viability of HL-60 cells, increases the dATP concentration and induces apoptogenic cytochrome c release from mitochondria with concomitant elevation of caspase-9 activity. Our results indicate that use of dAMP derivatives with masked phosphate may be a feasible approach for pharmacological elevation of intracellular dATP and induction of apoptosis. Topics: Annexin A5; Apoptosis; Caspase 9; Caspases; Cell Survival; Cytochromes c; Deoxyadenine Nucleotides; DNA Fragmentation; Enzyme Activation; HL-60 Cells; Humans; Propidium	2006

Article

Year

Deoxy-adenosine-monophosphate (dAMP) di-n-butylester induces apoptosis by increasing the dATP level in HL-60 cells.

Cancer letters, 2006, Apr-28, Volume: 235, Issue:2

Deoxy-ATP is a potent inducer of apoptosis. We intended to synthesize a lipophilic dAMP derivative which, according to our working hypothesis penetrates into the cell, is converted to dAMP by intracellular esterases and to dATP by nucleotide kinases. We synthesized dAMP-di-n-butylester (DAB) and tested it. We found that it fulfills the above-described expectations. DAB treatment decreases the viability of HL-60 cells, increases the dATP concentration and induces apoptogenic cytochrome c release from mitochondria with concomitant elevation of caspase-9 activity. Our results indicate that use of dAMP derivatives with masked phosphate may be a feasible approach for pharmacological elevation of intracellular dATP and induction of apoptosis.

Topics: Annexin A5; Apoptosis; Caspase 9; Caspases; Cell Survival; Cytochromes c; Deoxyadenine Nucleotides; DNA Fragmentation; Enzyme Activation; HL-60 Cells; Humans; Propidium

2006