cytochalasin-d and wiskostatin

cytochalasin-d has been researched along with wiskostatin* in 1 studies

Other Studies

1 other study(ies) available for cytochalasin-d and wiskostatin

Article	Year
Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine. Veterinary microbiology, 2007, Mar-10, Volume: 120, Issue:3-4 Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly. Topics: Animals; Carbazoles; Cell Line; Colony Count, Microbial; Cytochalasin D; Electric Impedance; Epithelial Cells; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Intestine, Small; Lovastatin; Nucleic Acid Synthesis Inhibitors; Propanolamines; Salmonella Infections, Animal; Salmonella typhimurium; Swine; Swine Diseases; Time Factors	2007

Article

Year

Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine.

Veterinary microbiology, 2007, Mar-10, Volume: 120, Issue:3-4

Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.

Topics: Animals; Carbazoles; Cell Line; Colony Count, Microbial; Cytochalasin D; Electric Impedance; Epithelial Cells; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Intestine, Small; Lovastatin; Nucleic Acid Synthesis Inhibitors; Propanolamines; Salmonella Infections, Animal; Salmonella typhimurium; Swine; Swine Diseases; Time Factors

2007