cytochalasin-d and berbamine

cytochalasin-d has been researched along with berbamine* in 1 studies

Other Studies

1 other study(ies) available for cytochalasin-d and berbamine

ArticleYear
Integrity of actin-network is involved in uridine 5'-triphosphate evoked store-operated Ca2+ entry in bovine adrenocortical fasciculata cells.
    Journal of pharmacological sciences, 2003, Volume: 91, Issue:1

    Store-operated Ca(2+) entry channels (SOCs) play an important role in the regulation of diverse non-excitable cell functions. However, the precise mechanism of SOCs activation is still controversial. Uridine 5'-triphosphate (UTP) was shown to induce Ca(2+) entry in a dihydropyridines-insensitive manner and accelerated steroidogenesis in bovine adrenocortical fasciculata cells (BAFCs) via the Gq/11 protein-coupled P2Y(2) receptor. Therefore we investigated whether UTP is involved in SOCs activation and the mechanism of UTP-induced SOCs activation. Fura 2-loaded BAFCs were used for the measurement of intracellular concentration of Ca(2+) ([Ca(2+)](i)) mobilization. Extracellular UTP evoked Ca(2+) release from intracellular stores followed by an increase in Ca(2+) entry. The Ca(2+) influx elicited by UTP was inhibited not by nifedipine, but by Zn(2+), Cd(2+), and Ni(2+) (potency order: Zn(2+) > Cd(2+) >> Ni(2+)), and the effect of UTP was also attenuated by a phospholipase C inhibitor (U73122). These results indicate that UTP activates SOCs in BAFCs. The increase in [Ca(2+)](i) by UTP was attenuated by ML-9, a myosin-light chain kinase inhibitor, and calmodulin inhibitors, W-7 and E6 berbamine, in a concentration-dependent manner. These reagents depolymerized actin filaments with rhodamine staining in BAFCs. Cytochalasin D also inhibited UTP-activated SOCs and depolymerized actin filaments. From these results, we proposed that calcium/calmodulin dependent myosin-light chain kinase is involved in the mobilization of actin filaments and the integrity of actin-network plays an important role in UTP-induced SOCs activation in BAFCs.

    Topics: Actins; Alkaloids; Animals; Azepines; Benzylisoquinolines; Calcium; Calcium Channel Blockers; Cations; Cattle; Cells, Cultured; Cytochalasin D; Enzyme Inhibitors; Estrenes; Fluorescent Dyes; Indoles; Myosin-Light-Chain Kinase; Nucleic Acid Synthesis Inhibitors; Pyrrolidinones; Sulfonamides; Thapsigargin; Uridine Triphosphate; Zona Fasciculata

2003