cytellin and tiliroside

To study the chemical constituents from Mitrasacme pygmaea.. The compounds were isolated and purified by column chromatography and their structure were identified by NMR and MS,and comparison spectral data with literature.. Eleven compounds were isolated and identified as tricin-7-O-β-D-glucopyranoside( 1),massonianoid A( 2),kaempferol( 3),cinnamic acid( 4),quercetin( 5),tiliroside( 6),tricin( 7),β-sitosterol( 8),adenosine( 9),α-tocopherolquinone( 10)and β-daucosterol( 11).. All the compounds are isolated from this genus for the first time.

Topics: Flavonoids; Kaempferols; Magnoliaceae; Quercetin; Sitosterols

In order to isolate substances that inhibit the hemolytic activity of human serum against erythrocytes, we have evaluated whole plants of the Orostachys japonicus species with regard to its anti-complement activity, and have identified its active principles following activity-guided isolation. A methanol extract of the O. japonicus, as well as its n-hexane soluble fraction, exhibited significant anti-complement activity on the complement system, which was expressed as total hemolytic activity. A bioassay-guided chromatographic separation of the constituents resulted in the isolation of three known compounds 1-3 from the active n-hexane fraction. The structure of these compounds were analyzed, and they were identified as hydroxyhopanone (1), beta-sitosteryl-3-O-beta-D-glucopyranosyl-6'-O-palmitate (2), and beta-sitosteryl-3-O-beta-D-glucopyranoside (3), respectively. Of these compounds, compound 2 exhibited potent anti-complement activity (IC50= 1.0 +/- 0.1 microM) on the classical pathway of the complement, as compared to tiliroside (IC50= 76.5 +/- 1.1 microM), which was used as a positive control. However, compounds 1 and 3 exhibited no activity in this system.

Topics: Benzopyrans; Complement Inactivating Agents; Complement Pathway, Classical; Crassulaceae; Dose-Response Relationship, Drug; Flavonoids; Glucosides; Humans; In Vitro Techniques; Inhibitory Concentration 50; Korea; Male; Palmitates; Plant Extracts; Sitosterols