cytellin has been researched along with stigmasterol-glucoside* in 10 studies
10 other study(ies) available for cytellin and stigmasterol-glucoside
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Isolation, characterization and quantitative HPLC-DAD analysis of components of charantin from fruits of Momordica charantia.
Charantin, a steroidal glycoside, exists as a mixture of stigmasterol glucoside (STG) and β-sitosterol glucoside (BSG) in the fruits of Momordica charantia. Charantin has anti-diabetic activity comparable to insulin. The present work discusses a method for separation of components of charantin namely STG and BSG by simple extraction technique followed by preparative HPLC. The identity of separated components was established by chromatographic as well as spectral techniques. Also reversed phase HPLC-DAD method was developed and validated for estimation of STG and BSG present in fruits of Momordica charantia. The method used C18 column (75 mm × 4.6 mm, 3.5 µm) as stationary phase and methanol: water (98:02, v/v) as mobile phase. Retention times of STG and BSG were found to be 10.707 min and 11.870 min, respectively. The validated method was applied to evaluate content of these components in different extracts and some commercial herbal formulations containing fruits of Momordica charantia. Topics: Chromatography, High Pressure Liquid; Fruit; Glucosides; Momordica charantia; Sitosterols; Stigmasterol | 2021 |
Effects of Fermentation Conditions Using Lactobacillus plantarum on the Charantin, Stigmasterol Glucoside and β-sitosterol Glucoside Contents of Bitter Gourd (Momordica charantia L.) Juice.
Bitter gourd fruits contain high amounts of charantin, stigmasterol glucoside and β-sitosterol glucoside, which have been shown to provide health benefits for humans. However, the bitterness of the fruit means they are rarely consumed. This study aimed to assess the effects of Lactobacillus plantarum fermentation, which has previously been reported to effectively reduce bitterness, on the contents of these compounds. The current results suggest that Lactobacillus plantarum fermentation should be considered as a potential approach to enhance the levels of these compounds in bitter gourd juice. Topics: Fermentation; Glucosides; Humans; Lactobacillus plantarum; Momordica charantia; Sitosterols; Stigmasterol | 2020 |
Ichnocarpus frutescens (L.) R. Br. root derived phyto-steroids defends inflammation and algesia by pulling down the pro-inflammatory and nociceptive pain mediators: An in-vitro and in-vivo appraisal.
Topics: Analgesics; Anti-Inflammatory Agents; Apocynaceae; Cytokines; Fatty Acids, Unsaturated; Glucosides; Humans; Inflammation; Interleukin-6; Leukocytes, Mononuclear; Lipopolysaccharides; Macrophages; Molecular Docking Simulation; Nociceptive Pain; Pain Perception; Plant Extracts; Plant Roots; Sitosterols; Steroids; Stigmasterol; Tumor Necrosis Factor-alpha | 2018 |
In vitro effects of cholesterol β-D-glucoside, cholesterol and cycad phytosterol glucosides on respiration and reactive oxygen species generation in brain mitochondria.
The cluster of neurodegenerative disorders in the western Pacific termed amyotrophic lateral sclerosis-parkinsonism dementia complex (ALS-PDC) has been repeatedly linked to the use of seeds of various species of cycad. Identification and chemical synthesis of the most toxic compounds in the washed cycad seeds, a variant phytosteryl glucosides, and even more toxic cholesterol β-D-glucoside (CG), which is produced by the human parasite Helicobacter pylori, provide a possibility to study in vitro the mechanisms of toxicity of these compounds. We studied in detail the effects of CG on the respiratory activities and generation of reactive oxygen species (ROS) by nonsynaptic brain and heart mitochondria oxidizing various substrates. The stimulatory effects of CG on respiration and ROS generation showed strong substrate dependence, suggesting involvement of succinate dehydrogenase (complex II). Maximal effects on ROS production were observed with 1 μmol CG/1 mg mitochondria. At this concentration the cycad toxins β-sitosterol-β-D-glucoside and stigmasterol-β-D-glucoside had effects on respiration and ROS production similar to CG. However, poor solubility precluded full concentration analysis of these toxins. Cholesterol, stigmasterol and β-sitosterol had no effect on mitochondrial functions studied at concentrations up to 100 μmol/mg protein. Our results suggest that CG may influence mitochondrial functions through changes in the packing of the bulk membrane lipids, as was shown earlier by Deliconstantinos et al. (Biochem Cell Biol 67:16-24, 1989). The neurotoxic effects of phytosteryl glucosides and CG may be associated with increased oxidative damage of neurons. Unlike heart mitochondria, in activated neurons mitochondria specifically increase ROS production associated with succinate oxidation (Panov et al., J Biol Chem 284:14448-14456, 2009). Topics: Animals; Brain; Cell Respiration; Cholesterol; Dimethyl Sulfoxide; Glucosides; Hydrogen Peroxide; In Vitro Techniques; Mitochondria; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Sitosterols; Stigmasterol | 2010 |
Antiplasmodial and other constituents from four Indonesian Garcinia spp.
Phytochemical investigations of four Garcinia spp. from Indonesia, i.e. Garcinia griffithii T. Anderson, Garcinia celebica L., Garcinia cornea L. and Garcinia cymosa K. Schum (Clusiaceae), have resulted in the isolation of a xanthone, 1,5-dihydroxy-3,6-dimethoxy-2,7-diprenylxanthone, 1,7-dihydroxyxanthone, isoxanthochymol, beta-sitosterol-3-O-beta-D-glucoside and stigmasterol-3-O-beta-D-glucoside from the stem bark of G. griffithii; friedelin and 3beta-hydroxy-23-oxo-9,16-lanostadien-26-oic acid or garcihombronane D from leaves of G. celebica; 23-hydroxy-3-oxo-cycloart-24-en-26-oic acid and epicatechin from stem bark of G. cornea; (+/-)-morelloflavone, morelloflavone-7-O-beta-D-glucoside or fukugiside, the triterpene 3beta-hydroxy-5-glutinen-28-oic acid and canophyllol from stem bark of G. cymosa. The xanthone and garcihombronane D displayed a selective activity against Plasmodium falciparum; isoxanthochymol and the triterpene beta-hydroxy-5-glutinen-28-oic acid a broad but non-selective antiprotozoal activity. Topics: Animals; Antimalarials; Antiprotozoal Agents; Benzophenones; Catechin; Flavones; Garcinia; Glucosides; Indonesia; Lanosterol; Molecular Structure; Plant Bark; Plant Leaves; Plants, Medicinal; Plasmodium falciparum; Sitosterols; Stigmasterol; Triterpenes; Xanthones | 2009 |
Withanolides from Withania somnifera roots.
Two new and seven known withanolides along with beta-sitosterol, stigmasterol, beta-sitosterol glucoside, stigmasterol glucoside, alpha+beta glucose were isolated from the roots of Withania somnifera. Among the known compounds, Viscosa lactone B, stigmasterol, stigmasterol glucoside and alpha+beta glucose are being reported from the roots of W. somnifera for the first time. One of the new compounds contained the rare 16beta-acetoxy-17(20)-ene the other contained unusual 6alpha-hydroxy-5,7alpha-epoxy functional groups in the withasteroid skeleton. The structures were elucidated by spectroscopic methods and chemical transformations. Topics: Glucosides; Molecular Structure; Plant Extracts; Plant Roots; Sitosterols; Stigmasterol; Withania; Withanolides | 2008 |
[Study on sterols and triterpenes from the stems of Akebia quinata].
The ethanol extract from Akebia quinata was seperated by dichloromethane, n-butanol in sequence. Six compounds were isolated and identified as beta-sitosterol (I), delta(5,22) stigmasterol (II), oleanolic acid (III), hederagenin (IV), daucosterol (V), delta(5,22) stigmasterol-3-O-beta-D-glycopyranoside (VI). Compound VI was isolated from Akebia plants for the first time. Topics: Glucosides; Magnoliopsida; Molecular Structure; Oleanolic Acid; Plant Stems; Plants, Medicinal; Sitosterols; Sterols; Stigmasterol; Triterpenes | 2005 |
A new 3,4-seco-lupane derivative from Lasianthus gardneri.
A new seco-ring A lupane triterpene derivative (1), along with lupenone, lupeol, beta-sitosterol, ursolic acid, and stigmasterol 3-O-beta-d-glucoside, were isolated from a methanol extract of mature stems of Lasianthus gardneri, a shrub from the family Rubiaceae growing in Sri Lanka. The structure and stereochemistry of the new compound were determined using a combination of (13)C and (1)H homo- and heteronuclear 2D NMR experiments and from mass spectral data. The structure of 1 was confirmed by partial synthesis from lupeol. Topics: Glucosides; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Pentacyclic Triterpenes; Plant Stems; Rubiaceae; Sitosterols; Sri Lanka; Stigmasterol; Triterpenes; Ursolic Acid | 2004 |
Isolation of various forms of sterol beta-D-glucoside from the seed of Cycas circinalis: neurotoxicity and implications for ALS-parkinsonism dementia complex.
The factors responsible for ALS-parkinsonism dementia complex (ALS-PDC), the unique neurological disorder of Guam, remain unresolved, but identification of causal factors could lead to clues for related neurodegenerative disorders elsewhere. Earlier studies focused on the consumption and toxicity of the seed of Cycas circinalis, a traditional staple of the indigenous diet, but found no convincing evidence for toxin-linked neurodegeneration. We have reassessed the issue in a series of in vitro bioassays designed to isolate non-water soluble compounds from washed cycad flour and have identified three sterol beta-d-glucosides as potential neurotoxins. These compounds give depolarizing field potentials in cortical slices, induce alterations in the activity of specific protein kinases, and cause release of glutamate. They are also highly toxic, leading to release of lactate dehydrogenase (LDH). Theaglycone form, however, is non-toxic. NMDA receptor antagonists block the actions of the sterol glucosides, but do not compete for binding to the NMDA receptor. The most probable mechanism leading to cell death may involve glutamate neuro/excitotoxicity. Mice fed cycad seed flour containing the isolated sterol glucosides show behavioral and neuropathological outcomes, including increased TdT-mediated biotin-dUTP nick-end labelling (TUNEL) positivity in various CNS regions. Astrocytes in culture showed increased caspase-3 labeling after exposure to sterol glucosides. The present results support the hypothesis that cycad consumption may be an important factor in the etiology of ALS-PDC and further suggest that some sterol glucosides may be involved in other neurodegenerative disorders. Topics: Amyotrophic Lateral Sclerosis; Animals; Astrocytes; Biological Assay; Cells, Cultured; Cerebral Cortex; Cholesterol; Cycas; Dementia; Glucose; Glucosides; Guam; Humans; In Vitro Techniques; Male; Mice; Neurons; Neurotoxins; Parkinsonian Disorders; Patch-Clamp Techniques; Phytosterols; Plant Extracts; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; Seeds; Sitosterols; Stigmasterol | 2002 |
[Study on the constituents from Neonauclea sessilifolia].
Seven compounds were isolated from Neonauclea sessilifolia. They are paeonol, 3,4-dihydroxybenzoic acid, scopoletin, anthraquinnones chrysophanol, 4H-1-benzopyran-4-one,5,7-dihydroxy-2-methyl, beta-sitosterol and stigmasterol glucoside. These compounds were isolated at first time from this genus and this plant. Topics: Acetophenones; Gas Chromatography-Mass Spectrometry; Glucosides; Hydroxybenzoates; Plant Stems; Plants, Medicinal; Rubiaceae; Scopoletin; Sitosterols; Stigmasterol | 2002 |