cytellin and stigmast-7-enol

Terpenoids from natural plant sources are valuable for their diverse biological activities that have important roles in the medical and agrochemical industries. In this study, we assessed the antioxidant, antifungal, and aphicidal activities of a mixture of spinasterol and 22,23-dihydrospinasterol from the leaves of Citrullus colocynthis. We used 1,1-diphenyl-2-picrylhydrazyl (DPPH) to assess antioxidant activity, and we measured antifungal activity using mycelium growth inhibition assays with three pathogenic fungi, Magnaporthe grisea, Rhizoctonia solani, and Phytophthora infestans. Aphicidal activity against adults of Myzus persicae was determined using in vitro and in vivo assays. Spinasterol and 22,23-dihydrospinasterol exhibited moderate antioxidant activity, even at lower concentrations: 19.98% at 0.78 µg mL

Topics: Antifungal Agents; Antioxidants; Citrullus colocynthis; Plant Leaves; Sitosterols; Stigmasterol; Triterpenes

The aqueous extract of. The aqueous extract from fruit of. Chemical fingerprint showed five majoritarian compounds in the aqueous extract of. Five compounds were identified in

Topics: Alloxan; Animals; Benzyl Alcohols; Coumaric Acids; Cucurbita; Diabetes Mellitus, Experimental; Glucosides; Hydroxybenzoates; Hypoglycemic Agents; Insulin; Liver; Liver Glycogen; Male; Mice; Phytochemicals; Phytotherapy; Plant Extracts; Propionates; Sitosterols

Spinasterol and schottenol, two phytosterols present in argan oil and in cactus pear seed oil, were synthesized from commercially available stigmasterol by a four steps reactions. In addition, the effects of these phytosterols on cell growth and mitochondrial activity were evaluated on 158N murine oligodendrocytes, C6 rat glioma cells, and SK-N-BE human neuronal cells with the crystal violet test and the MTT test, respectively. The effects of spinasterol and schottenol were compared with 7-ketocholesterol (7KC) and ferulic acid, which is also present in argan and cactus pear seed oil. Whatever the cells considered, dose dependent cytotoxic effects of 7KC were observed whereas no or slight effects of ferulic acid were found. With spinasterol and schottenol, no or slight effects on cell growth were detected. With spinasterol, reduced mitochondrial activities (30-50%) were found on 158N and C6 cells; no effect was found on SK-N-BE. With schottenol, reduced mitochondrial activity were revealed on 158N (50%) and C6 (10-20%) cells; no effect was found on SK-N-BE. Altogether, these data suggest that spinasterol and schottenol can modulate mitochondrial activity and might therefore influence cell metabolism.

Topics: Animals; Cell Line; Cell Proliferation; Central Nervous System; Humans; Mice; Mitochondria; Phytosterols; Plant Oils; Pyrus; Rats; Seeds; Sitosterols; Stigmasterol

In this study, we present the difference in sterol composition of extracted steryl glycosides (SG) hydrolyzed by either enzymatic or acid hydrolysis. SG were analyzed from foods belonging to the plant families Cucurbitaceae (melon and pumpkin seeds) and Amaranthaceae (amaranth and beetroot), both of which are dominated by Δ(7)-sterols. Released sterols were quantified by gas chromatography with a flame ionization detector (GC-FID) and identified using gas chromatography/mass spectrometry (GC-MS). All Δ(7)-sterols identified (Δ(7)-stigmastenyl, spinasteryl, Δ(7)-campesteryl, Δ(7)-avenasteryl, poriferasta-7,25-dienyl and poriferasta-7,22,25-trienyl glucoside) underwent isomerization under acidic conditions and high temperature. Sterols with an ethylidene or methylidene side chain were found to form multiple artifacts. The artifact sterols coeluted with residues of incompletely isomerized Δ(7)-sterols, or Δ(5)-sterols if present, and could be identified as Δ(8(14))-sterols on the basis of relative retention time, and their MS spectra as trimethylsilyl (TMS) and acetate derivatives. For instance, SG from melon were composed of 66% Δ(7)-stigmastenol when enzymatic hydrolysis was performed, whereas with acid hydrolysis only 8% of Δ(7)-stigmastenol was determined. The artifact of Δ(7)-stigmastenol coeluted with residual non-isomerized spinasterol, demonstrating the high risk of misinterpretation of compositional data obtained after acid hydrolysis. Therefore, the accurate composition of SG from foods containing sterols with a double bond at C-7 can only be obtained by enzymatic hydrolysis or by direct analysis of the intact SG.

Topics: Acids; Beta vulgaris; Biocatalysis; Cucurbitaceae; Food Analysis; Gas Chromatography-Mass Spectrometry; Glycosides; Hydrolysis; Sitosterols; Sterols

The objective of this study was to evaluate the biological activities of the major phytosterols present in argan oil (AO) and in cactus seed oil (CSO) in BV2 microglial cells. Accordingly, we first determined the sterol composition of AO and CSO, showing the presence of Schottenol and Spinasterol as major sterols in AO. While in CSO, in addition to these two sterols, we found mainly another sterol, the Sitosterol. The chemical synthesis of Schottenol and Spinasterol was performed. Our results showed that these two phytosterols, as well as sterol extracts from AO or CSO, are not toxic to microglial BV2 cells. However, treatments by these phytosterols impact the mitochondrial membrane potential. Furthermore, both Schottenol and Spinasterol can modulate the gene expression of two nuclear receptors, liver X receptor (LXR)-α and LXRβ, their target genes ABCA1 and ABCG1. Nonetheless, only Schottenol exhibited a differential activation vis-à-vis the nuclear receptor LXRβ. Thus Schottenol and Spinasterol can be considered as new LXR agonists, which may play protective roles by the modulation of cholesterol metabolism.

Topics: Animals; ATP Binding Cassette Transporter 1; ATP Binding Cassette Transporter, Subfamily G, Member 1; ATP-Binding Cassette Transporters; Cell Line; Gene Expression Regulation; Lipoproteins; Liver X Receptors; Membrane Potential, Mitochondrial; Mice; Microglia; Opuntia; Orphan Nuclear Receptors; Plant Oils; Seeds; Sitosterols; Sterols; Stigmasterol

Three crystals have been isolated from the root of Berneuxia thibetica. On the basis of spectral data, crystals A and B have been identified as chrysosplenetin B and 22,23-dihydrospinasterone respectively, while crystal C is a mix of spinasterol and 22,23-dihydrospinasterol.

Topics: Drugs, Chinese Herbal; Flavonoids; Sitosterols; Stigmasterol

Four steroids were isolated from the Saussurea gossypiphora for the fist time. They were determined as 3-stigmastanol, beta-sitosterol, stigmast-7-en-3-ol and ergostan-3,24-diol by spectral and chemical methods.

Topics: Drugs, Chinese Herbal; Ergosterol; Sitosterols

Nine compounds were isolated from the pericarp of T. rosthornii and identified on the basis of spectral data to be palmitic acid, lignoceric acid, cerotic acid, montanic acid, melissic acid, L-(-)-alpha-monopalmitin, delta 7-stigmastenol, delta 7-stigmastenone-3 and delta 7-stigmatenol-3-beta-D-glucopyranoside. All but the last one are obtained from this species for the first time.

Topics: Drugs, Chinese Herbal; Glycerides; Palmitic Acids; Sitosterols

Topics: Plant Extracts; Plants, Medicinal; Sitosterols; Stigmasterol